LOCUS       40924_35359        9502 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pPP1mds1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9502)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9502)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 9502)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             954..2525
                     /codon_start=1
                     /note="unnamed protein product; mds1"
                     /protein_id="SJL87800.1"
                     /translation="MRFPSSSVLALGLIGPALAYPKPGATKRGSPNPTRAAAVKAAFQT
                     SWNAYHHFAFPHDDLHPVSNSFDDERNGWGSSAIDGLDTAILMGDADIVNTILQYVPQI
                     NFTTTAVANQGSSVFETNIRYLGGLLSAYDLLRGPFSSLATNQTLVNSLLRQAQTLANG
                     LKVAFTTPSGVPDPTVFFNPTVRRSGASSNNVAEIGSLVLEWTRLSDLTGNPQYAQLAQ
                     KGESYLLNPKGSPEAWPGLIGTFVSTSNGTFQDSSGSWSGLMDSFYEYLIKMYLYDPVA
                     FAHYKDRWVLGADSTIGHLGSHPSTRKDLTFLSSYNGQSTSPNSGHLASFGGGNFILGG
                     ILLNEQKYIDFGIKLASSYFGTYTQTASGIGPEGFAWVDSVTGAGGSPPSSQSGFYSSA
                     GFWVTAPYYILRPETLESLYYAYRVTGDSKWQDLAWEALSAIEDACRAGSAYSSINDVT
                     QANGGGASDDMESFWFAEALKYAYLIFAEESDVQVQATGGNKFVFNTEAHPFSIRSSSR
                     RGGHLA"
     terminator      2800..3046
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(3459..5993)
                     /codon_start=1
                     /note="unnamed protein product; HIS4"
                     /protein_id="SJL87801.1"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     misc_feature    6348..7104
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    7247..7387
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7573..8161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8335..9192)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9193..9297)
                     /label=AmpR promoter
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tcctgctgcc aacatgagat
      961 tccctagcag ctccgtcctt gccctcgggc tcatcggacc tgcgctggcg tatccaaagc
     1021 cgggcgccac aaaacgtgga tctcccaacc ctacgagggc ggcagcagtc aaggccgcat
     1081 tccagacgtc gtggaacgct taccaccatt ttgcctttcc ccatgacgac ctccacccgg
     1141 tcagcaacag ctttgatgat gagagaaacg gctggggctc gtcggcaatc gatggcttgg
     1201 acacggctat cctcatgggg gatgccgaca ttgtgaacac gatccttcag tatgtaccgc
     1261 agatcaactt caccacgact gcggttgcca accaaggatc ctccgtgttc gagaccaaca
     1321 ttcggtacct cggtggcctg ctttctgcct atgacctgtt gcgaggtcct ttcagctcct
     1381 tggcgacaaa ccagaccctg gtaaacagcc ttctgaggca ggctcaaaca ctggccaacg
     1441 gcctcaaggt tgcgttcacc actcccagcg gtgtcccgga ccctaccgtc ttcttcaacc
     1501 ctactgtccg gagaagtggt gcatctagca acaacgtcgc tgaaattgga agcctggtgc
     1561 tcgagtggac acggttgagc gacctgacgg gaaacccgca gtatgcccag cttgcgcaga
     1621 agggcgagtc gtatctcctg aatccaaagg gaagcccgga ggcatggcct ggcctgattg
     1681 gaacgtttgt cagcacgagc aacggtacct ttcaggatag cagcggcagc tggtccggcc
     1741 tcatggacag cttctacgag tacctgatca agatgtacct gtacgacccg gttgcgtttg
     1801 cacactacaa ggatcgctgg gtccttggtg ccgactcgac cattgggcat ctcggctctc
     1861 acccgtcgac gcgcaaggac ttgacctttt tgtcttcgta caacggacag tctacgtcgc
     1921 caaactcagg acatttggcc agttttggcg gtggcaactt catcttggga ggcattctcc
     1981 tgaacgagca aaagtacatt gactttggaa tcaagcttgc cagctcgtac tttggcacgt
     2041 acacccagac ggcttctgga atcggccccg aaggcttcgc gtgggtggac agcgtgacgg
     2101 gcgccggcgg ctcgccgccc tcgtcccagt ccgggttcta ctcgtcggca ggattctggg
     2161 tgacggcacc gtattacatc ctgcggccgg agacgctgga gagcttgtac tacgcatacc
     2221 gcgtcacggg cgactccaag tggcaggacc tggcgtggga agcgttgagt gccattgagg
     2281 acgcatgccg cgccggcagc gcgtactcgt ccatcaacga cgtgacgcag gccaacggcg
     2341 ggggtgcctc tgacgatatg gagagcttct ggtttgccga ggcgctcaag tatgcgtacc
     2401 tgatctttgc ggaggagtcg gatgtgcagg tgcaggccac cggcgggaac aaatttgtct
     2461 ttaacacgga ggcgcacccc tttagcatcc gttcatcatc acgacggggc ggccaccttg
     2521 cttaaaggcg ctgagagttc tctcgcctgt ttttttgctc gtattattca ttttctgctg
     2581 ctcgtcccga atcgctccgt agatattttg acacatgcgt ctggatagag cggctgggac
     2641 caccgaatat ggtatgtcat tcgagatatg aagcgcaaga gatccccggg taccgagctc
     2701 gaattcccta gggcggccgc gaattaattc gccttagaca tgactgttcc tcagttcaag
     2761 ttgggcactt acgagaagac cggtcttgct agattctaat caagaggatg tcagaatgcc
     2821 atttgcctga gagatgcagg cttcattttt gatacttttt tatttgtaac ctatatagta
     2881 taggattttt tttgtcattt tgtttcttct cgtacgagct tgctcctgat cagcctatct
     2941 cgcagctgat gaatatcttg tggtaggggt ttgggaaaat cattcgagtt tgatgttttt
     3001 cttggtattt cccactcctc ttcagagtac agaagattaa gtgagaagtt cgtttgtgca
     3061 agcttatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg
     3121 caccgtgtat gaaatctaac aatgcgctca tcgtcatcct cggcaccgtc accctggatg
     3181 ctgtaggcat aggcttggtt atgccggtac tgccgggcct cttgcgggat atcgtccatt
     3241 ccgacagcat cgccagtcac tatggcgtgc tgctagcgct atatgcgttg atgcaatttc
     3301 tatgcgcacc cgttctcgga gcactgtccg accgctttgg ccgccgccca gtcctgctcg
     3361 cttcgctact tggagccact atcgactacg cgatcatggc gaccacaccc gtcctgtgga
     3421 tctatcgaat ctaaatgtaa gttaaaatct ctaaataatt aaataagtcc cagtttctcc
     3481 atacgaacct taacagcatt gcggtgagca tctagacctt caacagcagc cagatccatc
     3541 actgcttggc caatatgttt cagtccctca ggagttacgt cttgtgaagt gatgaacttc
     3601 tggaaggttg cagtgttaac tccgctgtat tgacgggcat atccgtacgt tggcaaagtg
     3661 tggttggtac cggaggagta atctccacaa ctctctggag agtaggcacc aacaaacaca
     3721 gatccagcgt gttgtacttg atcaacataa gaagaagcat tctcgatttg caggatcaag
     3781 tgttcaggag cgtactgatt ggacatttcc aaagcctgct cgtaggttgc aaccgatagg
     3841 gttgtagagt gtgcaataca cttgcgtaca atttcaaccc ttggcaactg cacagcttgg
     3901 ttgtgaacag catcttcaat tctggcaagc tccttgtctg tcatatcgac agccaacaga
     3961 atcacctggg aatcaatacc atgttcagct tgagacagaa ggtctgaggc aacgaaatct
     4021 ggatcagcgt atttatcagc aataactaga acttcagaag gcccagcagg catgtcaata
     4081 ctacacaggg ctgatgtgtc attttgaacc atcatcttgg cagcagtaac gaactggttt
     4141 cctggaccaa atattttgtc acacttagga acagtttctg ttccgtaagc catagcagct
     4201 actgcctggg cgcctcctgc tagcacgata cacttagcac caaccttgtg ggcaacgtag
     4261 atgacttctg gggtaagggt accatccttc ttaggtggag atgcaaaaac aatttctttg
     4321 caaccagcaa ctttggcagg aacacccagc atcagggaag tggaaggcag aattgcggtt
     4381 ccaccaggaa tatagaggcc aactttctca ataggtcttg caaaacgaga gcagactaca
     4441 ccagggcaag tctcaacttg caacgtctcc gttagttgag cttcatggaa tttcctgacg
     4501 ttatctatag agagatcaat ggctctctta acgttatctg gcaattgcat aagttcctct
     4561 gggaaaggag cttctaacac aggtgtcttc aaagcgactc catcaaactt ggcagttagt
     4621 tctaaaaggg ctttgtcacc attttgacga acattgtcga caattggttt gactaattcc
     4681 ataatctgtt ccgttttctg gataggacga cgaagggcat cttcaatttc ttgtgaggag
     4741 gccttagaaa cgtcaatttt gcacaattca atacgacctt cagaagggac ttctttaggt
     4801 ttggattctt ctttaggttg ttccttggtg tatcctggct tggcatctcc tttccttcta
     4861 gtgaccttta gggacttcat atccaggttt ctctccacct cgtccaacgt cacaccgtac
     4921 ttggcacatc taactaatgc aaaataaaat aagtcagcac attcccaggc tatatcttcc
     4981 ttggatttag cttctgcaag ttcatcagct tcctccctaa ttttagcgtt caacaaaact
     5041 tcgtcgtcaa ataaccgttt ggtataagaa ccttctggag cattgctctt acgatcccac
     5101 aaggtggctt ccatggctct aagacccttt gattggccaa aacaggaagt gcgttccaag
     5161 tgacagaaac caacacctgt ttgttcaacc acaaatttca agcagtctcc atcacaatcc
     5221 aattcgatac ccagcaactt ttgagttgct ccagatgtag cacctttata ccacaaaccg
     5281 tgacgacgag attggtagac tccagtttgt gtccttatag cctccggaat agactttttg
     5341 gacgagtaca ccaggcccaa cgagtaatta gaagagtcag ccaccaaagt agtgaataga
     5401 ccatcggggc ggtcagtagt caaagacgcc aacaaaattt cactgacagg gaactttttg
     5461 acatcttcag aaagttcgta ttcagtagtc aattgccgag catcaataat ggggattata
     5521 ccagaagcaa cagtggaagt cacatctacc aactttgcgg tctcagaaaa agcataaaca
     5581 gttctactac cgccattagt gaaacttttc aaatcgccca gtggagaaga aaaaggcaca
     5641 gcgatactag cattagcggg caaggatgca actttatcaa ccagggtcct atagataacc
     5701 ctagcgcctg ggatcatcct ttggacaact ctttctgcca aatctaggtc caaaatcact
     5761 tcattgatac cattattgta caacttgagc aagttgtcga tcagctcctc aaattggtcc
     5821 tctgtaacgg atgactcaac ttgcacatta acttgaagct cagtcgattg agtgaacttg
     5881 atcaggttgt gcagctggtc agcagcatag ggaaacacgg cttttcctac caaactcaag
     5941 gaattatcaa actctgcaac acttgcgtat gcaggtagca agggaaatgt catacttgaa
     6001 gtcggacagt gagtgtagtc ttgagaaatt ctgaagccgt atttttatta tcagtgagtc
     6061 agtcatcagg agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     6121 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     6181 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     6241 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     6301 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg agtatctatg
     6361 attggaagta tgggaatggt gatacccgca ttcttcagtg tcttgaggtc tcctatcaga
     6421 ttatgcccaa ctaaagcaac cggaggagga gatttcatgg taaatttctc tgacttttgg
     6481 tcatcagtag actcgaactg tgagactatc tcggttatga cagcagaaat gtccttcttg
     6541 gagacagtaa atgaagtccc accaataaag aaatccttgt tatcaggaac aaacttcttg
     6601 tttcgaactt tttcggtgcc ttgaactata aaatgtagag tggatatgtc gggtaggaat
     6661 ggagcgggca aatgcttacc ttctggacct tcaagaggta tgtagggttt gtagatactg
     6721 atgccaactt cagtgacaac gttgctattt cgttcaaacc attccgaatc cagagaaatc
     6781 aaagttgttt gtctactatt gatccaagcc agtgcggtct tgaaactgac aatagtgtgc
     6841 tcgtgttttg aggtcatctt tgtatgaata aatctagtct ttgatctaaa taatcttgac
     6901 gagccaaggc gataaatacc caaatctaaa actcttttaa aacgttaaaa ggacaagtat
     6961 gtctgcctgt attaaacccc aaatcagctc gtagtctgat cctcatcaac ttgaggggca
     7021 ctatcttgtt ttagagaaat ttgcggagat gcgatatcga gaaaaaggta cgctgatttt
     7081 aaacgtgaaa tttatctcaa gatctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7141 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7201 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7261 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7321 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7381 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7441 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7501 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7561 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7621 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7681 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7741 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7801 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7861 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7921 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7981 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8041 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8101 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8161 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8221 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8281 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8341 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8401 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8461 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8521 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8581 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8641 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8701 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8761 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8821 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8881 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8941 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9001 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9061 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9121 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9181 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9241 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9301 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     9361 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttaattctca tgtttgacag
     9421 cttatcatcg ataagctgac tcatgttggt attgtgaaat agacgcagat cgggaacact
     9481 gaaaaataac agttattatt cg
//
