LOCUS       40924_35344       11891 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPOP6IPT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Schafer M, Brutting C, Gase K, Reichelt M, Baldwin I, Meldau S.
  TITLE     'Real time' genetic manipulation: a new tool for ecological field 
            studies
  JOURNAL   Plant J. 76 (3), 506-518 (2013)
  PUBMED    23906159
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   Gase K, Meldau S, Schaefer M, Baldwin IT.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUN-2012) Molecular Ecology, Max-Planck-Institute for 
            Chemical Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, 
            Germany
REFERENCE   3  (bases 1 to 11891)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11891)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2013"; volume: "76"; issue: "3"; pages: "506-518"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUN-2012) Molecular Ecology, Max-Planck-Institute for Chemical 
            Ecology, Hans-Knoell-Strasse 8, Jena, Thueringen 07745, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      complement(1..345)
                     /note="Agrobacterium tumefaciens isopentenyl
                     phosphotransferase"
                     /regulatory_class="terminator"
     CDS             complement(420..1442)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1494..1673)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(2094..2110)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2118..2134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2142..2172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2187..2208)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2496..3084)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3371..4162)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(4266..4271)
                     /label=Transposon Tn5
                     /note="Transposon Tn5"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(4289..4294)
                     /label=Transposon Tn5
                     /note="Transposon Tn5"
                     /regulatory_class="minus_35_signal"
     primer_bind     4667..4683
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4729..4748
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4781..4800
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4833..4852
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4885..4904
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    4937..4956
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    complement(4989..5008)
                     /label=lac operator (symmetric)
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        5032..5078
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             5111..5833
                     /codon_start=1
                     /product="IPT"
                     /label=IPT
                     /note="Agrobacterium tumefaciens isopentenyl transferase"
                     /protein_id="AGI38108.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     regulatory      5899..6058
                     /label=cauliflower mosaic virus
                     /note="cauliflower mosaic virus"
                     /regulatory_class="terminator"
     misc_feature    6125..6149
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7303..7497)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7566..8636)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9068..9694)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    11178..11202
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
ORIGIN
        1 gtacccgaaa tataaacaac ttgtaatgat gtatttatat ttcataaaag cgctatattt
       61 aatatttatt gcgttctatg atatatatta aacatttaga caaattatac ataacacgca
      121 cacttacgat agtttatttt tacaattata gccacattat acatcgaaat tttaaaattt
      181 aacataatac tttcgtggaa actaagactt gttcaccagc ataaagtttg aagacacgat
      241 ggattgtcgg aagtatttgc cgccctgcca caacaggaca aagccaaaaa caattacgaa
      301 catcttatcc agatgaagac aggtgcgacg cagggctggc gtaacctaat acattccgaa
      361 cggatgacct tcgaatccgt cgaaagcggc tgcgttgatc ccggtcggca tctactctat
      421 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
      481 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
      541 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
      601 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc
      661 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
      721 caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac
      781 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
      841 gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
      901 agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
      961 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     1021 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     1081 gcatccatgg cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct
     1141 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc
     1201 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     1261 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     1321 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     1381 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     1441 atatcttatt gccccccggg atcagatctg gattgagagt gaatatgaga ctctaattgg
     1501 ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc
     1561 tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag
     1621 ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg ttctgtcagt
     1681 tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg actcccttaa
     1741 ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     1801 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     1861 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     1921 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     1981 atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag
     2041 cccttgcgcc ctgagtgctt gcggcagcgt gaagctagct tggcgtaatc atggtcatag
     2101 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     2161 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     2221 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     2281 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2341 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2401 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     2461 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2521 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2581 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2641 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2701 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2761 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2821 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2881 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2941 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3001 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3061 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3121 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3181 acctagatcc ttttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     3241 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     3301 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     3361 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     3421 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     3481 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     3541 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     3601 gggtcacgac gagatcctcg ccgtcgggca tccgcgcctt gagcctggcg aacagttcgg
     3661 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     3721 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     3781 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     3841 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     3901 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     3961 atagccgcgc tgcctcgtct tggagttcat tcagggcacc ggacaggtcg gtcttgacaa
     4021 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     4081 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     4141 gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga tcagatcttg
     4201 atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact ttgcagggct
     4261 tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct gtccataaaa
     4321 ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt ctctttgcgc
     4381 ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt cagcaccgtt
     4441 tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc cctgagtgct
     4501 tgcggcagcg tgaagctagc ttatcgccat tcgccattca ggctgcgcaa ctgttgggaa
     4561 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4621 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4681 agtgaattcg agctcaagaa atctagaaag aagaaaggga agagaaagaa ttgtgagcgc
     4741 tcacaattga aagactagaa agaagaaagg gaagagaaag aattgtgagc gctcacaatt
     4801 gaaagactag aaagaagaaa gggaagagaa agaattgtga gcgctcacaa ttgaaagact
     4861 agaaagaaga aagggaagag aaagaattgt gagcgctcac aattgaaaga ctagaaagaa
     4921 gaaagggaag agaaagaatt gtgagcgctc acaattgaaa gactagaaag aagaaaggga
     4981 agagaaagaa ttgtgagcgc tcacaattga aagactagtg gatcgatctt cgcaagaccc
     5041 ttcctctata taaggaagtt catttcattt ggagaggaca cgctgaagct agcttatcga
     5101 taccgtcgac atggacctgc atctaatttt cggtccaact tgcacaggaa agacgacgac
     5161 cgcgatagct cttgcccagc agacagggct tccagtcctt tcgcttgatc gggtccaatg
     5221 ctgtcctcaa ctatcaaccg gaagcggacg accaacagtg gaagaactga aaggaacgac
     5281 gcgtctctac cttgatgatc ggcctctggt ggagggtatc atcgcagcca agcaagctca
     5341 tcataggctg atcgaggagg tgtataatca tgaggccaac ggcgggctta ttcttgaggg
     5401 aggatccacc tcgttgctca actgcatggc gcgaaacagc tattggagtg cagattttcg
     5461 ttggcatatt attcgccaca agttacccga ccaagagacc ttcatgaaag cggccaaggc
     5521 cagagttaag cagatgttgc accccgctgc aggccattct attattcaag agttggttta
     5581 tctttggaat gaacctcggc tgaggcccat tctgaaagag atcgatggat atcgatatgc
     5641 catgttgttt gctagccaga accagatcac ggcagatatg ctattgcagc ttgacgcaaa
     5701 tatggaaggt aagttgatta atgggatcgc tcaggagtat ttcatccatg cgcgccaaca
     5761 ggaacagaaa ttcccccaag ttaacgcagc cgctttcgac ggattcgaag gtcatccgtt
     5821 cggaatgtat tagcccgggg atcctctaga gtccgcaaaa atcaccagtc tctctctaca
     5881 aatctatctc tctctatttt tctccagaat aatgtgtgag tagttcccag ataagggaat
     5941 tagggttctt atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
     6001 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtga
     6061 cctgcaggca tgcaagcttg gatcagattg tcgtttcccg ccttcggttt aaactatcag
     6121 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc
     6181 ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac
     6241 cacagggttc ccctcgggag tgcttggcat tccgtgcgat aatgacttct gttcaaccac
     6301 ccaaacgtcg gaaagcctga cgacggagca gcattccaaa aagatccctt ggctcgtctg
     6361 ggtcggctag aaggtcgagt gggctgctgt ggcttgatcc ctcaacgcgg tcgcggacgt
     6421 agcgcagcgc cgaaaaatcc tcgatcgcaa atccgacgct gtcgaaaagc gtgatctgct
     6481 tgtcgctctt tcggccgacg tcctggccag tcatcacgcg ccaaagttcc gtcacaggat
     6541 gatctggcgc gagttgctgg atctcgcctt caatccgggt ctgtggcggg aactccacga
     6601 aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc
     6661 cgacgccgtt gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt
     6721 gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagagg
     6781 tgcaggcctc gatctgaaac ccgaaccgct ggagattgcg ggagcagcga gcagtagcct
     6841 cggggtcgat gtcgtaaagt cgtatccgat cgacgccgat cagcgccttg aaggccaaag
     6901 cctggaactc actttgggca ccgttgccga tcagcgccat cgtgcgcgaa tctttacggg
     6961 ccagatactt tgccgcgatc gcggaggtcg cggccgttcg caaggccgtc aggattgtca
     7021 tttccgacag cagcagcgga tagccgctat cgacatcgga gagcacgccg aacgcggtta
     7081 cctagagcgg ccgccaccgc ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     7141 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     7201 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     7261 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     7321 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     7381 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     7441 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     7501 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     7561 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     7621 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     7681 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     7741 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     7801 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     7861 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     7921 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     7981 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     8041 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     8101 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     8161 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     8221 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     8281 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     8341 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     8401 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     8461 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     8521 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     8581 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     8641 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     8701 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     8761 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     8821 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     8881 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     8941 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     9001 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     9061 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     9121 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     9181 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     9241 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     9301 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     9361 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     9421 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     9481 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     9541 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     9601 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     9661 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     9721 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     9781 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     9841 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     9901 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     9961 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    10021 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    10081 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    10141 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    10201 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    10261 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    10321 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    10381 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    10441 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    10501 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    10561 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    10621 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    10681 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    10741 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    10801 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    10861 aggggttgga tcgacctcga gggggggccc ggtaccgtga acgtcggctc gattgtacct
    10921 gcgttcaaat actttgcgat cgtgttgcgc gcctgcccgg tgcgtcggct gatctcacgg
    10981 atcgactgct tctctcgcaa cgccatccga cggatgatgt ttaaaagtcc catgtggatc
    11041 actccgttgc cccgtcgctc accgtgttgg ggggaaggtg cacatggctc agttctcaat
    11101 ggaaattatc tgcctaaccg gctcagttct gcgtagaaac caacatgcaa gctccaccgg
    11161 gtgcaaagcg gcagcggcgg caggatatat tcaattgtaa atggcttcat gtccgggaaa
    11221 tctacatgga tcagcaatga gtatgatggt caatatggag aaaaagaaag agtaattacc
    11281 aatttttttt caattcaaaa atgtagatgt ccgcagcgtt attataaaat gaaagtacat
    11341 tttgataaaa cgacaaatta cgatccgtcg tatttatagg cgaaagcaat aaacaaatta
    11401 ttctaattcg gaaatcttta tttcgacgtg tctacattca cgtccaaatg ggggcttaga
    11461 tgagaaactt cacgatcgat gccttgattt cgccattccc agatacccat ttcatcttca
    11521 gattggtctg agattatgcg aaaatataca ctcatataca taaatactga cagtttgagc
    11581 taccaattca gtgtagccca ttaccttaca taattcactc aaatgctagg cagtctgtca
    11641 actcggcgtc aatttgtcgg ccactatacg atagttgcgc aaattttcaa agtcctggcc
    11701 taacatcaca cctctgtcgg cggcgggtcc catttgtgat aaatccacca tcacaataga
    11761 tagtctaatg gacgaaaaag gcgaatattt cgatgctgag attcgacgca attaattcga
    11821 gaaaaatccc gtgattgatg ctgttgagtt accaataata tgggcagcga aggccattta
    11881 attataagat c
//
