LOCUS       40924_35339        5544 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPOP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Soderholm JF, Bevis BJ, Glick BS.
  TITLE     A vector for Pop-in/Pop-out gene replacement in Pichia pastoris
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5544)
  AUTHORS   Soderholm JF, Bevis BJ, Glick BS.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-NOV-2000) Molecular Genetics and Cell Biology, 
            University of Chicago, 920 E. 58th St., Chicago, IL 60637, USA
REFERENCE   3  (bases 1 to 5544)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5544)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-NOV-2000) Molecular Genetics and Cell Biology, University of 
            Chicago, 920 E. 58th St., Chicago, IL 60637, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(302..886)
                     /codon_start=1
                     /product="T-urf13"
                     /label=T-urf13
                     /protein_id="AAK19282.1"
                     /translation="MASTRVLASRLASQMAASAKVARPAVRVAQVSKRTIQTGSPLQTL
                     KRTQMTSIVNATTRQAFQKRAYSSEIAQAMARSGMITTFLNLPPFDQGLVFFGSIFIFF
                     LCILLIKGYLRKMDDSYLAQLSELANHNRVEAAKAGHVALHELSFSWLRGVQIRVRTLP
                     IQWNEGGGRSNDQSTLSKPKYSSMTDSVQVP"
     promoter        complement(900..1376)
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     primer_bind     1585..1601
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1602..1658
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1671..1687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1695..1711)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1719..1749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1764..1785)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2073..2661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2835..3692)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        3710..4025
                     /label=ARG4 promoter
     CDS             4026..5414
                     /codon_start=1
                     /label=ARG4
                     /note="argininosuccinate lyase, required for arginine 
                     biosynthesis"
                     /translation="MSDGTQKLWGGRFTGETDPLMHLYNASLPYDYKMYKADLEGTKVY
                     TAGLQKLGLLTETELAKIHEGLAEIKKEWDADKFVRHPNDEDIHTANERRLGELIGREI
                     AGKVHTGRSRNDQVVTDLRIYCRDIVNDTLFPALKGLVEVLIKRAEGEIDVLMPGYTHL
                     QRAQPIRWSHWLSSYATYFTEDYKRLGQILHRLNQSPLGAGALAGHPYGIDREFLAEGL
                     GFNSVIGNSLVAVSDRDFIVELMFWGTLFMNHISRFAEDLIIYCTAEFGFIQLSDAYST
                     GSSLMPQKKNADSLELLRGKSGRVFGDLTGFLMSLKGIPSTYDKDMQEDKEPLFDCLTT
                     VEHSMLIATGVISTLTVNKEKMEAALTMDMLATDLADYLVRKGVPFRETHHISGECVAT
                     AERLGLSGIDKLTLEQYQKIDSRFGQDLFETFNFEQSVERRDATGGTAKSAVLKQLDNL
                     KSQLN"
ORIGIN
        1 ggtcacatgt gtgggaaata ccaagaaaaa catcaaactc gaatgatttt cccaaacccc
       61 taccacaaga tattcatcag ctgcgagata ggctgatcag gagcaagctc gtacgagaag
      121 aaacaaaatg acaaaaaaaa tcctatacta tataggttac aaataaaaaa gtatcaaaaa
      181 tgaagcctgc atctctcagg caaatggcat tctgacatcc tcttgattag aatctagcaa
      241 gaccggtctt ctcgtaagtg cccaacttga actgaggaac agtcatgtct aagaagctct
      301 atcacggtac ttgtacgcta tcggtcattg aggaatactt aggcttagag agagtggatt
      361 ggtcgttgct tcgaccccct ccttcattcc attgtatagg taaggtcctc accctaattt
      421 gaacccccct caaccacgag aaggatagct catgcagggc cacgtggccc gcttttgccg
      481 cttccactct attgtggttg gctaactcgg agagttgagc caaataggaa tcatccattt
      541 tacggagata tccctttatc aataatatgc acaaaaaaaa aataaaaata gaaccgaaaa
      601 ataccaaacc ttgatcaaag ggaggaaggt ttaagaaagt agtgatcata ccggatctgg
      661 ccatggcctg ggcgatctcg gaagagtagg cgcgcttctg gaaagcctgg cgggtggtgg
      721 cgttgacgat ggaggtcatc tgggtgcgct tgagggtctg gaggggggag ccagtctgga
      781 tggtgcgctt gctgacctga gcaacgcgga cagcagggcg ggcaaccttg gcggaagcag
      841 ccatctggga ggccaggcga gaggcgagga cacgagtgga ggccattttg taattcttga
      901 tagttgttca attgattgaa atagggacaa ataaattaaa tttaaagtct ttgggtcagg
      961 agaaaccaaa attgggaaag gtgttcgcct tttatattcg attctggtgg tttccaataa
     1021 tctcatgaca tgcgtccgcc cgctattatt gccagcgacg gccgggactt ttccatccct
     1081 gggctgctag gtcgggtaca cgacctccgt tttacccgca acgtaatgct gggaagagca
     1141 ttgctgcaag ggggccgtag aagaagctct ccagcagagt aaaatttcct agggacggta
     1201 acgggcggtg gaaggagaga gaagggaaga gacgtttctg gttccattac tccacattta
     1261 agttttaccc cggagaattt tacgttgcca gcaggtcgtt cggagttgca acggagccag
     1321 atatttcaga gatggctacc tgattggacg aggacaccaa gacatttcta caaaaaagga
     1381 tcgactctag tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1441 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     1501 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     1561 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattcgag ctcggtaccc
     1621 ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     1681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     2101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     2161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     2281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     2521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     3241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata acgacaatca
     3721 aataacctga tctgccaagg ctccatcata tctggcctag aaacagtttt ttttttcgat
     3781 tattttgttc gttcttgtgg tggttactca ttggcagaat cccgaaaatc atgattagta
     3841 gatgaatgac tcactttttg gataagctgg cgcaaattga aacatgtgaa aaaaaaaaaa
     3901 aaggattata aaaggtcagc gaagcacaga actctgagat aagactacct ttctttagct
     3961 aggggagaat attcgcaatt gaagagctca aaagcaggta actatataac aagactaagg
     4021 caaacatgtc agacggcact caaaaactat ggggtgggag attcactggt gaaaccgatc
     4081 ctttgatgca cctttacaat gcgtctcttc cgtatgatta taagatgtat aaggcagatt
     4141 tagaaggaac taaagtatac acagcgggct tgcagaagtt gggtcttcta acggagacag
     4201 aattggcaaa gatccatgaa ggtttggctg aaatcaaaaa agaatgggac gctgacaaat
     4261 ttgtccgtca tccaaacgac gaggatatcc atactgcgaa tgaaagacgt cttggtgaac
     4321 taattggccg cgagattgct ggtaaagtcc acaccggtag atcccgtaat gatcaagttg
     4381 ttaccgattt gagaatatac tgtcgtgaca ttgtcaatga caccctcttt ccagctttaa
     4441 agggcttggt tgaagttcta attaagaggg ccgaaggtga gatagatgtc ttaatgccag
     4501 gctacacaca tttacaaagg gcacaaccta ttagatggtc tcattggttg agctcttatg
     4561 caacatactt caccgaagat tacaagagac tgggtcaaat actacacaga ttgaatcaat
     4621 caccactggg tgcaggcgct cttgctggtc atccttacgg cattgataga gaatttttgg
     4681 ctgaaggttt gggtttcaat agtgtaattg gtaactcctt ggttgctgtt tctgatagag
     4741 atttcatcgt ggagttgatg ttttggggaa ctttgttcat gaaccatatt tctcgttttg
     4801 ctgaagattt gattatatat tgtacagcag aatttggttt catacagttg agcgacgcct
     4861 attcaacagg ttcttcttta atgcctcaga agaagaatgc agactcgtta gagttgttaa
     4921 gaggtaaatc cggtagagta tttggtgatc tgacaggatt cttgatgagt ttgaagggta
     4981 tcccatctac ttatgataaa gacatgcaag aagacaaaga gccactattc gattgcttaa
     5041 caactgtaga gcactccatg ctgattgcca caggtgttat ttctacctta actgtaaata
     5101 aggaaaagat ggaagctgct ctcacgatgg atatgctagc taccgacttg gcagattact
     5161 tggtcagaaa gggtgttcca ttcagagaga ctcatcacat atctggtgag tgtgtcgcta
     5221 ctgctgaaag acttggtcta agcggtattg ataaattaac cttggagcag tatcaaaaga
     5281 tcgattcgag attcggacaa gatctttttg aaacttttaa ctttgaacaa agcgttgaaa
     5341 gacgagatgc tactggtgga accgctaaat ctgctgtatt gaagcaattg gataatttga
     5401 aatcccaatt aaattagcag atggcgacgt tatgcgcaag aatttcatca ggtctggtac
     5461 ttctaggact tcatcaaccc ttctttaaat acttattttg ttattagtat tatattttcg
     5521 agattgtgct atgtatatat atac
//
