LOCUS       40924_35314        6778 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPM7g, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6778)
  AUTHORS   Martins PM, Lau IF, Bacci M, Belasque J, do Amaral AM, Taboga SR, 
            Ferreira H.
  TITLE     Subcellular localization of proteins labeled with GFP in Xanthomonas
            citri ssp. citri: targeting the division septum
  JOURNAL   FEMS Microbiol. Lett. 310 (1), 76-83 (2010)
  PUBMED    20629754
REFERENCE   2  (bases 1 to 6778)
  AUTHORS   Martins PMM., do Amaral AM, Taboga SR, Lau I, Ferreira H.
  TITLE     Subcellular localization of proteins labelled with GFP in 
            Xanthomonas citri subsp. citri
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6778)
  AUTHORS   Martins PMM., Ferreira H, Lau I.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2010) Ciencias Farmaceuticas, UNESP, Rodovia 
            Araraquara-Jau Km1, Araraquara, SP 14801-902, Brazil
REFERENCE   4  (bases 1 to 6778)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6778)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2010"; volume: "310"; issue: "1"; pages: 
            "76-83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (05-MAR-2010) Ciencias Farmaceuticas, UNESP, Rodovia Araraquara-Jau 
            Km1, Araraquara, SP 14801-902, Brazil"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1050..2102)
                     /codon_start=1
                     /product="XylR"
                     /label=XylR
                     /note="xylose promoter (pxyl) repressor"
                     /protein_id="ADF80257.1"
                     /translation="MTGLNKSTVSSQVNTLMKESMVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSVGIDVGVDYINGILTDLEGTIVLDQYRHLESNSPEITKDILIDMIHHFITQMPQSP
                     YGFIGIGICVPGLIDKDQKIVFTPNSNWRDIDLKSSIQEKYNVSVFIENEANAGAYGEK
                     LFGAAKNHDNIIYVSISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKLSYQDIINLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAVILRNSIIESHPMVLNSMRSEVSSRVYSQLGNSYELLPSSLGQNAPALGMSSIV
                     IDHFLDMITM"
     CDS             2468..3181
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3237..3253)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3394..3822
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             4166..4957
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4978..5835
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6009..6597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcctgc
      241 tgcatgctgg cgccaatggc gaacgcgttg cgcaccgcat tgaacagcgg aaagtcgtag
      301 gccgcatgcg gcgtggattg caggtacggc gccaggaact ggtcgtaatc gccattgccc
      361 gtaccgccgc cggtgatcac ttcgccgaag acatacacac ccgatcggat gccggcatcg
      421 aagacgcgat tgagatggtc gaacgtcatg tgcttggccg catccacccg gaagccggtc
      481 acgcccatgc tcttgagcgc ctgcagatag gcgcgctgct gctgtaccac ccagtcgttg
      541 cccagcaaat ccggcaggcc tggatcgctg ccgccgccgc agatacggta attgcgcacc
      601 tggtaagcgt cgttgtagtt gctgatgcac tgcgccgggc cgaaatcgct ggcgctgagg
      661 aagtttgact gcagcgtgcc gaacaagcgc agcgaatcgt aacggccggg gtttgccgcg
      721 tactgcgaca ggaccgcgcc gcctgggtaa ttgaggtccg agcgtgtggc cgcttcgttg
      781 gccatgtggt tgaacacgat gtcggcatag gtttccacgc cgttgctggc cagcgcctgg
      841 accatcttct tgaatgcagc ggtgtcgccg agcggattgt cgatcaggcg gatgtcctgg
      901 ggttgatagc gcgcccacca ggcacttcct tcggagcggt acgcgggggc gaccaatacc
      961 ttgcgatagc cggcatcggc aatctgcttg gcgcgcgctt ccacggtggc atacggccag
     1021 ttgaaggcgt gcaggatgac atcgaattct tacattgtaa tcatgtccag aaaatgatca
     1081 atcacaatgg aggacattcc taatgccggt gcattctgtc ctaaggaaga tggcaataat
     1141 tcatagctat tgcctaattg ggaataaacc cttgatgata cttcacttct cattgaattt
     1201 aaaaccatag gatgcgattc aattatgcta tttcttaaaa ttacggcttg tgggttgaaa
     1261 gtatttagaa tattggtaag gcctattcct aaatagaatc caaaattttg taatgcattt
     1321 aaggttccga tatcattcag atgggcgagg tttatgatat cttgatagga cagttttttc
     1381 tctttggtct gaagagattt taataaagcc ttctctgaag catacaattc ccagcatcct
     1441 cggtttccgc aactgcattt aggaccatta aagtctattg tcatatgtcc catttctcca
     1501 gagaagccgc ttactcctct atataaatga ttgttgataa taacaccgat ccctattcct
     1561 gtgctgatac ttacgtaaat aatgttatcg tgattttttg cagctccaaa tagtttttct
     1621 ccatatgcgc cagcatttgc ctcattttca ataaaaacag acacattgta cttctcttgt
     1681 atcgaagatt ttaagtcaat atctctccag ttggagttcg gagtgaaaac aattttttga
     1741 tctttatcaa tgagtccagg cacgcaaata cctataccaa taaacccgta cggagattgg
     1801 ggcatttgcg taataaagtg atgaatcata tcaatcaaaa tgtctttcgt tatttctgga
     1861 gaattggatt ccaaatggcg gtattgatca agaacgattg ttccttcaag gtctgttaaa
     1921 atgccattaa tataatccac accaacatct attccaacgg agtatcctgc ctttttatta
     1981 aaaacaagca tgacaggtct tcttccgcca cttgattgtc cttgacctat ttcaaatacc
     2041 atactttctt tcattaacgt gtttacctgt gatgagacag ttgatttatt taatccagtc
     2101 atttcagata attttgctct tgaaataggt gaatttttaa ggatttcttt taataataac
     2161 ttttgattta cttttttgac aaaggtttga tcagcgatat ccacttcatc cactccattt
     2221 gtttaatctt taaattaagt atcaacatag tacatagcga atcttccctt tattaaatct
     2281 aatgtgttca taaaaaacta aaaaaaatat tgaaaatact gacgaggtta tataagatga
     2341 aaataagtta gtttgtttaa acaacaaact aataggtgat gtacttacta tatgaaataa
     2401 aatgcatctg tatttgaatg aatttattaa gcttgggaaa aggaggtgag gatccatggc
     2461 actcgagatg agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     2521 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     2581 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
     2641 aacacttgtc actactttcg cgtatggtct tcaatgcttt gcgagatacc cagatcatat
     2701 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     2761 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     2821 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     2881 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
     2941 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
     3001 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     3061 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
     3121 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     3181 agaagcaggc ggccgcgcac tgaatctaga aagctagctg acccgggaag cttggcactg
     3241 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     3301 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     3361 tcccaacagt tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg
     3421 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3481 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3541 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3601 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3661 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3721 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3781 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa attcagggcg
     3841 caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc
     3901 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     3961 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     4021 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     4081 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatctgatca
     4141 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     4201 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     4261 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     4321 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     4381 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4441 gctattgggc gaagtgccgg ggcaggatct cctgtcatcc caccttgctc ctgccgagaa
     4501 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4561 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4621 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4681 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4741 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4801 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4861 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4921 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attgaaaaag gaagagtatg
     4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     5161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     5821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     6121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     6661 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     6721 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
