LOCUS       40924_35309        6759 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pPM2a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Martins PM, Lau IF, Bacci M, Belasque J, do Amaral AM, Taboga SR, 
            Ferreira H.
  TITLE     Subcellular localization of proteins labeled with GFP in Xanthomonas
            citri ssp. citri: targeting the division septum
  JOURNAL   FEMS Microbiol. Lett. 310 (1), 76-83 (2010)
  PUBMED    20629754
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Martins PMM., Ferreira H, Lau I.
  TITLE     An expression vector for GFP-tagging of proteins in Xanthomonas 
            axonopodis pv. citri
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6759)
  AUTHORS   Martins PMM., Ferreira H, Lau I.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAY-2009) UNESP, Rua Cristovao Colombo, 2265, Sao Jose
            do Rio Preto, SP 15054000, Brazil
REFERENCE   4  (bases 1 to 6759)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6759)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2010"; volume: "310"; issue: "1"; pages: 
            "76-83"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-MAY-2009) UNESP, Rua Cristovao Colombo, 2265, Sao Jose do Rio 
            Preto, SP 15054000, Brazil"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1050..2102)
                     /codon_start=1
                     /gene="xylR"
                     /product="pxyl promoter repressor"
                     /label=xylR
                     /protein_id="ACS28252.1"
                     /translation="MTGLNKSTVSSQVNTLMKESMVFEIGQGQSSGGRRPVMLVFNKKA
                     GYSVGIDVGVDYINGILTDLEGTIVLDQYRHLESNSPEITKDILIDMIHHFITQMPQSP
                     YGFIGIGICVPGLIDKDQKIVFTPNSNWRDIDLKSSIQEKYNVSVFIENEANAGAYGEK
                     LFGAAKNHDNIIYVSISTGIGIGVIINNHLYRGVSGFSGEMGHMTIDFNGPKCSCGNRG
                     CWELYASEKALLKSLQTKEKKLSYQDIINLAHLNDIGTLNALQNFGFYLGIGLTNILNT
                     FNPQAVILRNSIIESHPMVLNSMRSEVSSRVYSQLGNSYELLPSSLGQNAPALGMSSIV
                     IDHFLDMITM"
     gene            complement(1050..2102)
                     /gene="xylR"
                     /label=xylR
     CDS             2456..3169
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3218..3234)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3375..3803
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             4147..4938
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4959..5816
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcctgc
      241 tgcatgctgg cgccaatggc gaacgcgttg cgcaccgcat tgaacagcgg aaagtcgtag
      301 gccgcatgcg gcgtggattg caggtacggc gccaggaact ggtcgtaatc gccattgccc
      361 gtaccgccgc cggtgatcac ttcgccgaag acatacacac ccgatcggat gccggcatcg
      421 aagacgcgat tgagatggtc gaacgtcatg tgcttggccg catccacccg gaagccggtc
      481 acgcccatgc tcttgagcgc ctgcagatag gcgcgctgct gctgtaccac ccagtcgttg
      541 cccagcaaat ccggcaggcc tggatcgctg ccgccgccgc agatacggta attgcgcacc
      601 tggtaagcgt cgttgtagtt gctgatgcac tgcgccgggc cgaaatcgct ggcgctgagg
      661 aagtttgact gcagcgtgcc gaacaagcgc agcgaatcgt aacggccggg gtttgccgcg
      721 tactgcgaca ggaccgcgcc gcctgggtaa ttgaggtccg agcgtgtggc cgcttcgttg
      781 gccatgtggt tgaacacgat gtcggcatag gtttccacgc cgttgctggc cagcgcctgg
      841 accatcttct tgaatgcagc ggtgtcgccg agcggattgt cgatcaggcg gatgtcctgg
      901 ggttgatagc gcgcccacca ggcacttcct tcggagcggt acgcgggggc gaccaatacc
      961 ttgcgatagc cggcatcggc aatctgcttg gcgcgcgctt ccacggtggc atacggccag
     1021 ttgaaggcgt gcaggatgac atcgaattct tacattgtaa tcatgtccag aaaatgatca
     1081 atcacaatgg aggacattcc taatgccggt gcattctgtc ctaaggaaga tggcaataat
     1141 tcatagctat tgcctaattg ggaataaacc cttgatgata cttcacttct cattgaattt
     1201 aaaaccatag gatgcgattc aattatgcta tttcttaaaa ttacggcttg tgggttgaaa
     1261 gtatttagaa tattggtaag gcctattcct aaatagaatc caaaattttg taatgcattt
     1321 aaggttccga tatcattcag atgggcgagg tttatgatat cttgatagga cagttttttc
     1381 tctttggtct gaagagattt taataaagcc ttctctgaag catacaattc ccagcatcct
     1441 cggtttccgc aactgcattt aggaccatta aagtctattg tcatatgtcc catttctcca
     1501 gagaagccgc ttactcctct atataaatga ttgttgataa taacaccgat ccctattcct
     1561 gtgctgatac ttacgtaaat aatgttatcg tgattttttg cagctccaaa tagtttttct
     1621 ccatatgcgc cagcatttgc ctcattttca ataaaaacag acacattgta cttctcttgt
     1681 atcgaagatt ttaagtcaat atctctccag ttggagttcg gagtgaaaac aattttttga
     1741 tctttatcaa tgagtccagg cacgcaaata cctataccaa taaacccgta cggagattgg
     1801 ggcatttgcg taataaagtg atgaatcata tcaatcaaaa tgtctttcgt tatttctgga
     1861 gaattggatt ccaaatggcg gtattgatca agaacgattg ttccttcaag gtctgttaaa
     1921 atgccattaa tataatccac accaacatct attccaacgg agtatcctgc ctttttatta
     1981 aaaacaagca tgacaggtct tcttccgcca cttgattgtc cttgacctat ttcaaatacc
     2041 atactttctt tcattaacgt gtttacctgt gatgagacag ttgatttatt taatccagtc
     2101 atttcagata attttgctct tgaaataggt gaatttttaa ggatttcttt taataataac
     2161 ttttgattta cttttttgac aaaggtttga tcagcgatat ccacttcatc cactccattt
     2221 gtttaatctt taaattaagt atcaacatag tacatagcga atcttccctt tattaaatct
     2281 aatgtgttca taaaaaacta aaaaaaatat tgaaaatact gacgaggtta tataagatga
     2341 aaataagtta gtttgtttaa acaacaaact aataggtgat gtacttacta tatgaaataa
     2401 aatgcatctg tatttgaatg aatttattaa gcttgggaaa aggaggtgag gatccatgag
     2461 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     2521 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     2581 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     2641 tactttcgcg tatggtcttc aatgctttgc gagataccca gatcatatga aacagcatga
     2701 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     2761 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     2821 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     2881 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     2941 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     3001 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     3061 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     3121 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaag aagcaggcgg
     3181 ccgcgcactg aatctagaat gacccgggaa gcttggcact ggccgtcgtt ttacaacgtc
     3241 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     3301 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     3361 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3421 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3481 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3541 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3601 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3661 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     3721 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     3781 ttaacaaaaa tttaacgcga attttaacaa aattcagggc gcaagggctg ctaaaggaag
     3841 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     3901 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     3961 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     4021 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     4081 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     4141 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4201 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4261 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4321 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4381 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4441 gggcaggatc tcctgtcatc ccaccttgct cctgccgaga aagtatccat catggctgat
     4501 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4561 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4621 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     4681 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4741 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4801 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4861 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4921 cttcttgacg agttcttctg aattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4981 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5041 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5101 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5161 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5221 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5281 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5341 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5401 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5461 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5521 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5581 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5641 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5701 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5761 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5821 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5881 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
