LOCUS       V004031                 3198 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004031
VERSION     V004031
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3198)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    98..130
                     /label="5' UTR of MS2 polymerase, incl. RBS"
                     /note="5' UTR of MS2 polymerase, incl. RBS"
     CDS             131..679
                     /gene="C"
                     /label="Capsid protein"
                     /note="Capsid protein from Hepatitis B virus genotype D
                     subtype ayw (isolate France/Tiollais/1979). Accession#:
                     P03146"
     regulatory      686..725
                     /label="synthetic polyA"
                     /note="synthetic polyA"
                     /regulatory_class="terminator"
     rep_origin      complement(965..1553)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1727..2584)
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      2950..3080
                     /label="lambda PL promoter"
                     /note="lambda PL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
       61 tccctcagca atcgcagcaa actccggcat ctactaatag acgccggcca ttcaaacatg
      121 aggattaccc atggatatcg atccttataa agaattcgga gctactgtgg agttactctc
      181 gtttctcccg agtgacttct ttccttcagt acgagacctt ctggataccg ccagcgcgct
      241 gtatcgggaa gccttggagt ctcctgagca ctgcagccct caccatactg ccctcaggca
      301 agcaattctt tgctgggggg agctcatgac tctggccacg tgggtgggtg ttaacttgga
      361 agatccagct agcagggacc tggtagtcag ttatgtcaac actaatatgg gtttaaagtt
      421 caggcaactc ttgtggtttc acattagctg cctcactttc ggccgagaaa cagttctaga
      481 atatttggtg tctttcggag tgtggatccg cactcctcca gcttataggc ctccgaatgc
      541 ccctatcctg tcgacactcc cggagactac tgttgttaga cgtcgaggca ggtcacctag
      601 aagaagaact ccttcgcctc gcaggcgaag gtctcaatcg ccgcggcgcc gaagatctca
      661 atctcgggaa tctcaatgtt agtgaagctt ccgggccggc gataatacgc cggcccgttt
      721 tttttcagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
      781 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
      841 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
      901 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
      961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1381 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     1801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     1861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     1921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     1981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2341 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2581 tcatactctt cctttttcaa tattatgtaa gcagacagtt ttattgttca tgatgatata
     2641 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
     2701 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
     2761 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
     2821 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg gtatgagtca
     2881 gcaacacctt cttcacgagg cagacctcag cgccggtgat gccggccacg atgcgtccgg
     2941 cgtagaggat ctctcaccta ccaaacaatg cccccctgca aaaaataaat tcatataaaa
     3001 aacatacaga taaccatctg cggtgataaa ttatctctgg cggtgttgac ataaatacca
     3061 ctggcggtga tactgagcac atcagcagga cgcactgacc accatgaagg tgacgctctt
     3121 aaaattaagc cctgaagaag ggcagcattc aaagcagaag gctttggggt gtgtgatacg
     3181 aaacgaagca ttggaata
//
