LOCUS       40924_34949        3493 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pPLc300, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3493)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3493)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      362..410
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      469..550
                     /label=fdT
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      472..520
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      648..1103
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      complement(1262..1850)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2024..2881)
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      3247..3377
                     /label=PL
                     /note="PL"
                     /regulatory_class="promoter"
ORIGIN
        1 ttcagcacct gcacatggga cgtcgacctg aggtaattat aacccgggcc ctatatatgg
       61 atccaattgc aatgatcatc atgacagatc tgcgcgcgat cgatatcagc gctttaaatt
      121 tgcgcatgct agctatagtt ctagaggtac cggttgttaa cgttagccgg ctacgtatac
      181 tccggaatat taataggcct aggatgcata tggcggccgc ctgcagctgg cgccatcgat
      241 acgcgtacgt cgcgaccgcg gacatgtaca gagctcgaga agtactagtg gccacgtggg
      301 ccgtgcacct taagcttggc cgcgctagag gtcgaaattc acctcgaaag caagctgata
      361 aaccgataca attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa
      421 aaattattat tcgcaattcc aagctaattc acctcgaaag caagctgata aaccgataca
      481 attaaaggct ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat
      541 tcgcaattcc aagctctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      601 gcagctcccg gagacggtca cagcttgtct gtaagcggat gcagatcacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      781 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      841 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      901 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      961 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1021 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1081 caaaatatta acgtttacaa tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat
     1141 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1201 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1261 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1321 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1381 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1441 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1501 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1561 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1621 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1681 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1741 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1801 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1861 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1921 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1981 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2041 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2101 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2161 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2221 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2281 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2341 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2401 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2461 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2521 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2581 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
     2641 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2701 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2761 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2821 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2881 tactcttcct ttttcaatat tatgtaagca gacagtttta ttgttcatga tgatatattt
     2941 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttg ttgaataaat
     3001 cgaacttttg ctgagttgaa ggatcagatc acgcatcttc ccgacaacgc agaccgttcc
     3061 gtggcaaagc aaaagttcaa aatcaccaac tggtccacct acaacaaagc tctcatcaac
     3121 cgtggctccc tcactttctg gctggatgat ggggcgattc aggcctggta tgagtcagca
     3181 acaccttctt cacgaggcag acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt
     3241 agaggatctc tcacctacca aacaatgccc ccctgcaaaa aataaattca tataaaaaac
     3301 atacagataa ccatctgcgg tgataaatta tctctggcgg tgttgacata aataccactg
     3361 gcggtgatac tgagcacatc agcaggacgc actgaccacc atgaaggtga cgctcttaaa
     3421 attaagccct gaagaagggc agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa
     3481 cgaagcattg gaa
//
