LOCUS       V004034                 4708 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004034
VERSION     V004034
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4708)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4708)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..537
                     /label="5' UTR of phage T4 ligase, incl. RBS"
                     /note="5' UTR of phage T4 ligase, incl. RBS"
     CDS             538..1998
                     /gene="30"
                     /label="DNA ligase"
                     /note="DNA ligase from Enterobacteria phage T4. Accession#:
                     P00970"
     misc_feature    2151..2291
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2477..3065)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3239..4096)
                     /label="AmpR"
                     /note="beta-lactamase"
     regulatory      4462..4592
                     /label="PL promoter"
                     /note="PL promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 ttccggatcc ggccaagctt ttttcaccaa aggaaaataa agaaagtttt gatagtgaaa
       61 aagctataaa agatgtaatt gaaaagatga ttaaaaacga ttcttttcct tggaacacta
      121 cctggagaac tcctggattt aatccttata atccatatca tccatatcag acgcatccgt
      181 ttcagacatg gaactatatt aagcccggcg gtatagagta tttgtataat agacctactt
      241 gtggtgataa tattttccaa ggagcattct aatgtttgtt gttcacacta tttatgaaaa
      301 tgaaggtaat actacacgtg attacggtca cgtaaatcaa ttttttagat gcaatccaga
      361 attccgagct caaaaagacg aacgaatttt taaaaaatgt gtagagcaag gtttcattta
      421 tatcaagcac tggatgcaag gaaataaagt tagaaccacg taccacaggt ctttgactga
      481 gcttaatgat gaattgattt ataatagagc tgtaaaccaa actctaaagg atgaacaatg
      541 attcttaaaa ttctgaacga aatagcatct attggttcaa ctaaacagaa gcaagcaatt
      601 cttgaaaaga ataaagataa tgaattgctt aaacgagtat atcgtctgac ttattctcgt
      661 gggttacagt attatatcaa gaaatggcct aaacctggta ttgctaccca gagttttgga
      721 atgttgactc ttaccgatat gcttgacttc attgaattca cattagctac tcggaaattg
      781 actggaaatg cagcaattga ggaattaact ggatatatca ccgatggtaa aaaagatgat
      841 gttgaagttt tgcgtcgagt gatgatgcga gaccttgaat gtggtgcttc agtatctatt
      901 gcaaacaaag tttggccagg tttaattcct gaacaacctc aaatgctcgc aagttcttat
      961 gatgaaaaag gcattaataa gaatatcaaa tttccagcct ttgctcagtt aaaagctgat
     1021 ggagctcggt gttttgctga agttagaggt gatgaattag atgatgttcg tcttttatca
     1081 cgagctggta atgaatatct aggattagat cttcttaagg aagagttaat taaaatgacc
     1141 gctgaagccc gccagattca tccagaaggt gtgttgattg atggcgaatt ggtataccat
     1201 gagcaagtta aaaaggagcc agaaggccta gattttcttt ttgatgctta tcctgaaaac
     1261 agtaaagcta aagaattcgc cgaagtagct gaatcacgta ctgcttctaa tggaatcgcc
     1321 aataaatctt taaagggaac catttctgaa aaagaagcac aatgcatgaa gtttcaggtc
     1381 tgggattatg tcccgttggt agaaatatac agtcttcctg catttcgttt gaaatatgat
     1441 gtacgttttt ctaaactaga acaaatgaca tctggatatg ataaagtaat tttaattgaa
     1501 aaccaggtag taaataacct agatgaagct aaggtaattt ataaaaagta tattgaccaa
     1561 ggtcttgaag gtattattct caaaaatatc gatggattat gggaaaatgc tcgttcaaaa
     1621 aatctttata aatttaaaga agtaattgat gttgatttaa aaattgtagg aatttatcct
     1681 caccgtaaag accctactaa agcgggtgga tttattcttg agtcagagtg tggaaaaatt
     1741 aaggtaaatg ctggttcagg cttaaaagat aaagccggtg taaaatcgca tgaacttgac
     1801 cgtactcgca ttatggaaaa ccaaaattat tatattggaa aaattctaga gtgcgaatgc
     1861 aacggttggt taaaatctga tggccgcact gattacgtta aattatttct tccgattgcg
     1921 attcgtttac gtgaagataa aactaaagct aatacattcg aagatgtatt tggtgatttt
     1981 catgaggtaa ctggtctatg aaagcttggc tgcctcgcgc gtttcggtga tgacggtgaa
     2041 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     2101 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     2161 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     2221 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2281 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     2341 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     2401 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2461 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2521 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2581 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2641 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2701 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2761 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2821 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2881 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2941 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     3001 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     3061 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     3121 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     3181 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     3241 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     3301 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3361 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3421 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3481 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3541 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3601 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3661 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3721 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3781 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3841 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3901 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3961 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     4021 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     4081 aatgttgaat actcatactc ttcctttttc aatattatgt aagcagacag ttttattgtt
     4141 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     4201 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     4261 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     4321 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     4381 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgccggtg atgccggcca
     4441 cgatgcgtcc ggcgtagagg atctctcacc taccaaacaa tgcccccctg caaaaaataa
     4501 attcatataa aaaacataca gataaccatc tgcggtgata aattatctct ggcggtgttg
     4561 acataaatac cactggcggt gatactgagc acatcagcag gacgcactga ccaccatgaa
     4621 ggtgacgctc ttaaaattaa gccctgaaga agggcagcat tcaaagcaga aggctttggg
     4681 gtgtgtgata cgaaacgaag cattggaa
//
