LOCUS       40924_35279        9152 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLV30, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller
            B, Peris CL, Weijers D.
  TITLE     A versatile set of ligation-independent cloning vectors for 
            functional studies in plants
  JOURNAL   Plant Physiol. 156 (3), 1292-1299 (2011)
  PUBMED    21562332
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   De Rybel B, van den Berg W, Weijers D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen 
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 9152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2011"; volume: "156"; issue: "3"; pages: 
            "1292-1299"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2011) Laboratory of Biochemistry, Wageningen University, 
            Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(66..654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(828..1640)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1931..2366
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2501..2523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(2531..2707)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2812..3603)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3629..3974)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4176..4192
                     /note="M13F; M13 forward primer site"
     primer_bind     4196..4212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4219..4237
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4252..8364
                     /note="pMP; MONOPTEROS (ARF5) promoter"
                     /regulatory_class="promoter"
     misc_feature    8371..8400
                     /note="LIC; ligation-independent cloning (LIC) site"
     terminator      8426..8674
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(8711..8729)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8750..8766)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8774..8790)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8798..8828)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8843..8864)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    9103..9127
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
       61 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      541 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      781 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg
      841 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      901 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      961 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1021 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1081 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1141 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1201 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1261 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1321 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1381 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1441 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1501 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1561 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     1621 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1681 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1741 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     1801 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     1861 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     1921 caggcgatcc ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt
     1981 ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg
     2041 gctttccggc ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc
     2101 gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca
     2161 cgtgagaacg ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa
     2221 gtagttacag caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta
     2281 ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc
     2341 gcaagcggtt gcccaccgtc gagcgccagc gcctttgccc acaacccggc ggccggccgc
     2401 aacagatcgt tttataaatt tttttttttg aaaaagaaaa agcccgaaag gcggcaacct
     2461 ctcgggcttc tggatttccg atccccggaa ttagagatct tggcaggata tattgtggtg
     2521 taacgttatc gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     2581 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     2641 agcgaaaccc tataagaacc ctaatttccc ttatcgggaa actactcaca cattatttat
     2701 ggagaaaata gagagagata gatttgtaga gagagactgg tgatttcagc gtaccgaatt
     2761 gatcctctag ctagagtccg aaccccagag tcccgcacag agtcccgctc agaagaactc
     2821 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2881 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2941 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     3001 gccattttcc accatgatat tcggcaagca ggcatcgccc tgggtcacga cgagatcctc
     3061 gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     3121 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtcctcgctc
     3181 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     3241 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     3301 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     3361 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3421 ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3481 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3541 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3601 catcttgttc aatcatgcca gcttgggctg tcctctccaa atgaaatgaa cttccttata
     3661 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     3721 tgtcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     3781 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaatgatag
     3841 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttct actgtccttt
     3901 cgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgaa attacccttt
     3961 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgaca tttttggagt
     4021 aggggtacga taacattaac gtttacaatt tcgcgccatt cgccattcag gctgcgcaac
     4081 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     4141 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     4201 acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac cgacgtgtgt
     4261 gaattaccaa ggcgttgaac gacgtgaaag agcgcgtgcg gctcgattct tacgtcgttg
     4321 aatattgacc gtcggatcaa aactcatcga tccacataga tgcgtgcgcg aaggcacatg
     4381 ccgttatgaa gggttctcga tgaacatcac gtgtcctcta gggattggct attattgcaa
     4441 actccgtttt atgaggcaaa aagacaattt aaccttttta atttttatct atttggtaat
     4501 aaaaacattt ttatttcgtt tgtttattaa ttaattaatg aggttattaa aggtgcctta
     4561 gcaatagcag gtaggcactt aatttaatag tccaaactcc aaagctaatt aatttaaagg
     4621 acacaaatcc taataaaccc caaacagtta tttgcgacct tttcttatcg aaatccgttc
     4681 tttgaattgt ttactttttg atcgatttta ataacaagat tcacaaacac atataagtga
     4741 ttttttaaaa aatggaatga gaaaataaag aaaagaattt gaacggttaa gaatatgata
     4801 atcacgaaga tggcatgata ttatagttgg agtgggttac ttggcgaaag aatcaaatag
     4861 aattttcata aatttggaat gtgacgtgta cttatttctc caagcgttac ctgattttcg
     4921 tattattatt acggattcgt gatcttcgta tcccatatgg tcaatcgttt ctatttcact
     4981 tgtaaattac aatatctcgt tcactgttct ataacatact attacgtgct aagtattact
     5041 attgcacaga tcatcttgaa cggtaatcga cacttacccg ggttaatcag tattattact
     5101 ttcattattc taaccaaaaa ttaggttgca cttgcccatg tgtacgacac caattacctt
     5161 caaaaagttt cttcaaagta tcttcatcaa cttaacgtaa aattttaaac caacataaat
     5221 gtttttggtt agccaaaagt tagttaaaat ttcactacgt attatacatt tttaggttgc
     5281 taatcactta ttgttccact acatttttat tctttccaat tattctacat gttatatctc
     5341 cattttacat tacttggtcc actgtcactg ttaaaagcca ccactatttt atgttttttt
     5401 ttcaattact acgatttata agatttgtac tatattatgt tacaccaata tggaagtata
     5461 ctttttgtga tgatttaaga aagtaaatct agtggcagta agtaaaatta taatgaaact
     5521 gttttcttat gggattaata ggaatcggtc tttgctttgt tagtttggca ggagagagag
     5581 gtaggtaagg atgtgaactg tgaagcgcag aaacggcgcc gtgtcggtcc aaaaatcatt
     5641 ttcaaaataa cattaaaata cacgtgtcga gtatgctaat gtacgcctcc tcttcctctc
     5701 ttactcaacc attgatttgc cgtccacttc acgcgtgcct agcggtcaaa attttcagac
     5761 gcaaaagcac caaataatgc ttttcacttt ctttttttct ttcttcattt cttattcttg
     5821 tttttttaca agtttccaat ctacataaga caaactaacg tttaggaagg attcggattg
     5881 ataatctgca tacacttgat tttgattggt atccttttct ttgcatgtgg ttaaaatttg
     5941 gtatggtaag agtttttatt tttatgaatt taggattatt gaaggcattg tcacaaatga
     6001 tatacagtag agaaaaaaaa aaacgataca agagaaaaca attgataaga atgataaaat
     6061 tatatctagt aaacctcatc gtgggatgtt attcctcgtt atacgtaatg actatttgtt
     6121 ccaaagattt tgctgatggc acacaatctt ggcaccggaa atgagagttt ttccattatc
     6181 atcaaaagca tgtttcactc cttgctaaat ttgaactgta tattaaaatg tgctgccaac
     6241 agcaaattcc acacattata tacttgattg gagatccttt gattcaaata tatcttagtg
     6301 acaaacgcgt atccgaataa gtagttatgt ttatatagat attctatcat aatgatgcta
     6361 ctatatatcc acttataaat tataatatgt ttgattattt ttctctaata tgtgataatg
     6421 tggattgtgt actaatccta tatataactt aactatgctc atttgtattt tgtttatgag
     6481 taatattaca tgaaggaaag cgatttttat ttacgtatga ttgataagta atgatatgga
     6541 aagtgatatg gtttcatatc atacaatata atacaatgac cgcatgtcaa ttatttcgtc
     6601 gcaagaaaac aatggaaagg tcaaactaga gatgtgtgcg taaaaaaaaa aaatgtatgt
     6661 aactaaaaag gtctaaagat gtaatggtac acactataca caattagaca taattacaca
     6721 tgtggtttag agtattagat attaataatg agcagaacaa gctatgttta caggtgcaga
     6781 gaaccaagat attcctttct tttggttgtt gtagatacaa atatatttta caaagctcaa
     6841 tttcaataca aatgcaagaa ccacaaacaa cagtcctttc aatttatttg gaggtggtct
     6901 taactcttaa gtatatataa acaataccac cttataactt ttaagtttta actaaaccat
     6961 acttaaatct tttttaatct ctataccaaa tcttgcaaat atcaaaataa ctttttctaa
     7021 ccaaagaaag aaaacatttt tgaagtcctt aacttggaaa ggaaaagaac acccgggcaa
     7081 aaaagattgg aagatacact tcctcccgta acctctttag gcttcgtgct aatatacgct
     7141 attttgacat tctctcctat catacgcacc ttgtattaga gatctcccaa agtctcacca
     7201 ctcacatccc ttttacgaaa tctcagcaag aaaagattaa cattttgaga aaagtatata
     7261 ttattgcaac gaaaaaagaa gcctcctcct ttgtcataaa gttcttaatc aattttaatt
     7321 tattgccata atcaaaaaac attttatatt ttacaaagtt cttgaaaaag taggacactt
     7381 tttaaaataa tattcactca tggtacttat tttaagcttt attatctgcc ttataacata
     7441 agcattgaaa gtacttgtac attcaactac gtttctgtgc gtatcagaga ttacgtgtgc
     7501 ttactctact ttattaaatt tttgacgtaa actgttctaa aacattaaat aataatatag
     7561 atgtatctat tgaaatacca aaactttatt ttataattaa tgggaaacaa taaaatataa
     7621 tattattcgt ttaaagagtc taaagctgca acggcatcgc catatacaga aagttttaag
     7681 cgcaggataa gagcatgcac actctttccc atttccaagc aaaataaaac ctatacatac
     7741 acatttatac taatactcat atacattaga gagagaagaa gaaaaaatgt gggatttttc
     7801 agagaaataa ttaaaagaaa gcaaaagttt tttttttctt aaaagctttt gtctctctct
     7861 caattgcttc ttgttcttgt ttgcatccgg aaccaaagaa agaaactgtg tggtttcatg
     7921 atggcacctt tggtttctct tcttcattga agggtcgaat ttggtctgag aaaagaagag
     7981 tggagagaga gaaagagaag aggcaagagc cccacaagag agacatgctt gtctgtgtag
     8041 tccctatttt gtatgttctt taatctctcc cactctgttt ttgtcatgtg taagggtttt
     8101 gtccttcgat tttggagtct tctttaacca aaaaaaaaca gattccatga aatggctttg
     8161 tctttaattc ctctggtttg tcctggattt atcttcttgt agcctctttg ctcaatagag
     8221 ctctttttgt ctcttcttgt attaaataaa ataaaatgaa gacttagtaa agaaaaagaa
     8281 aaactcttga gtcttgctaa agcctagtta gtgttgagtg tgggagagac aaaacagaac
     8341 attgaaaaat ctctctgtat gatgctcgag ctagttggaa taggttaacc caactccata
     8401 ctgcagcccg ggggatccga tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     8461 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     8521 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     8581 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     8641 taaattatcg cgcgcggtgt catctatgtc tagagcggcc gccaccgcgg tggagctcca
     8701 gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg tcatagctgt
     8761 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8821 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8881 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8941 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9001 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9061 ccacagaatc aggggataac gcaggaaaga acatgaaggc cttgacagga tatattggcg
     9121 ggtaaactaa gtcgctgtat gtgtttgttt ga
//
