LOCUS       40924_35274        8799 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLV29, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller
            B, Peris CL, Weijers D.
  TITLE     A versatile set of ligation-independent cloning vectors for 
            functional studies in plants
  JOURNAL   Plant Physiol. 156 (3), 1292-1299 (2011)
  PUBMED    21562332
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   De Rybel B, van den Berg W, Weijers D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen 
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 8799)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2011"; volume: "156"; issue: "3"; pages: 
            "1292-1299"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2011) Laboratory of Biochemistry, Wageningen University, 
            Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(66..654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(828..1640)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1931..2366
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2501..2523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(2534..2786)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2823..3371)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(3413..3592)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     3819..3835
                     /note="M13F; M13 forward primer site"
     primer_bind     3839..3855
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3865..3883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3898..8010
                     /note="pMP; MONOPTEROS (ARF5) promoter"
                     /regulatory_class="promoter"
     misc_feature    8017..8046
                     /note="LIC; ligation-independent cloning (LIC) site"
     terminator      8072..8320
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(8357..8375)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8396..8412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(8420..8436)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8444..8475)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8490..8511)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    8750..8774
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
       61 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      541 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      781 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg
      841 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      901 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      961 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1021 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1081 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1141 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1201 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1261 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1321 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1381 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1441 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1501 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1561 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     1621 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1681 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1741 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     1801 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     1861 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     1921 caggcgatcc ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt
     1981 ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg
     2041 gctttccggc ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc
     2101 gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca
     2161 cgtgagaacg ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa
     2221 gtagttacag caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta
     2281 ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc
     2341 gcaagcggtt gcccaccgtc gagcgccagc gcctttgccc acaacccggc ggccggccgc
     2401 aacagatcgt tttataaatt tttttttttg aaaaagaaaa agcccgaaag gcggcaacct
     2461 ctcgggcttc tggatttccg atccccggaa ttagagatct tggcaggata tattgtggtg
     2521 taacgttatc cccgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     2581 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     2641 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     2701 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     2761 ctttattgcc aaatgtttga acgatcgagc ttgcatgctt gcagagatcc gccgggtcat
     2821 cagatttcgg tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc
     2881 cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccgcgg
     2941 ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca
     3001 gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg
     3061 gagcccagtc ccgtccgctg gtggcggggg gagacgtaca cggtcgactc ggccgtccag
     3121 tcgtaggcgt tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc
     3181 acctcggcga cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc
     3241 tgcggttcct gcggctcggt acggaagttg accgtgcttg tctcgatgta gtggttgacg
     3301 atggtgcaga ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt
     3361 tctgggctca tggtagatcc cctcgatcga gttgagagtg aatatgagac tctaattgga
     3421 taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct
     3481 gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc
     3541 tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt tctgtcagtt
     3601 ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat
     3661 tctccgctca tgatcgataa cattaacgct tacaatttcc attcgccatt caggctgcgc
     3721 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     3781 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     3841 aaaacgacgg ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtaccgac
     3901 gtgtgtgaat taccaaggcg ttgaacgacg tgaaagagcg cgtgcggctc gattcttacg
     3961 tcgttgaata ttgaccgtcg gatcaaaact catcgatcca catagatgcg tgcgcgaagg
     4021 cacatgccgt tatgaagggt tctcgatgaa catcacgtgt cctctaggga ttggctatta
     4081 ttgcaaactc cgttttatga ggcaaaaaga caatttaacc tttttaattt ttatctattt
     4141 ggtaataaaa acatttttat ttcgtttgtt tattaattaa ttaatgaggt tattaaaggt
     4201 gccttagcaa tagcaggtag gcacttaatt taatagtcca aactccaaag ctaattaatt
     4261 taaaggacac aaatcctaat aaaccccaaa cagttatttg cgaccttttc ttatcgaaat
     4321 ccgttctttg aattgtttac tttttgatcg attttaataa caagattcac aaacacatat
     4381 aagtgatttt ttaaaaaatg gaatgagaaa ataaagaaaa gaatttgaac ggttaagaat
     4441 atgataatca cgaagatggc atgatattat agttggagtg ggttacttgg cgaaagaatc
     4501 aaatagaatt ttcataaatt tggaatgtga cgtgtactta tttctccaag cgttacctga
     4561 ttttcgtatt attattacgg attcgtgatc ttcgtatccc atatggtcaa tcgtttctat
     4621 ttcacttgta aattacaata tctcgttcac tgttctataa catactatta cgtgctaagt
     4681 attactattg cacagatcat cttgaacggt aatcgacact tacccgggtt aatcagtatt
     4741 attactttca ttattctaac caaaaattag gttgcacttg cccatgtgta cgacaccaat
     4801 taccttcaaa aagtttcttc aaagtatctt catcaactta acgtaaaatt ttaaaccaac
     4861 ataaatgttt ttggttagcc aaaagttagt taaaatttca ctacgtatta tacattttta
     4921 ggttgctaat cacttattgt tccactacat ttttattctt tccaattatt ctacatgtta
     4981 tatctccatt ttacattact tggtccactg tcactgttaa aagccaccac tattttatgt
     5041 ttttttttca attactacga tttataagat ttgtactata ttatgttaca ccaatatgga
     5101 agtatacttt ttgtgatgat ttaagaaagt aaatctagtg gcagtaagta aaattataat
     5161 gaaactgttt tcttatggga ttaataggaa tcggtctttg ctttgttagt ttggcaggag
     5221 agagaggtag gtaaggatgt gaactgtgaa gcgcagaaac ggcgccgtgt cggtccaaaa
     5281 atcattttca aaataacatt aaaatacacg tgtcgagtat gctaatgtac gcctcctctt
     5341 cctctcttac tcaaccattg atttgccgtc cacttcacgc gtgcctagcg gtcaaaattt
     5401 tcagacgcaa aagcaccaaa taatgctttt cactttcttt ttttctttct tcatttctta
     5461 ttcttgtttt tttacaagtt tccaatctac ataagacaaa ctaacgttta ggaaggattc
     5521 ggattgataa tctgcataca cttgattttg attggtatcc ttttctttgc atgtggttaa
     5581 aatttggtat ggtaagagtt tttattttta tgaatttagg attattgaag gcattgtcac
     5641 aaatgatata cagtagagaa aaaaaaaaac gatacaagag aaaacaattg ataagaatga
     5701 taaaattata tctagtaaac ctcatcgtgg gatgttattc ctcgttatac gtaatgacta
     5761 tttgttccaa agattttgct gatggcacac aatcttggca ccggaaatga gagtttttcc
     5821 attatcatca aaagcatgtt tcactccttg ctaaatttga actgtatatt aaaatgtgct
     5881 gccaacagca aattccacac attatatact tgattggaga tcctttgatt caaatatatc
     5941 ttagtgacaa acgcgtatcc gaataagtag ttatgtttat atagatattc tatcataatg
     6001 atgctactat atatccactt ataaattata atatgtttga ttatttttct ctaatatgtg
     6061 ataatgtgga ttgtgtacta atcctatata taacttaact atgctcattt gtattttgtt
     6121 tatgagtaat attacatgaa ggaaagcgat ttttatttac gtatgattga taagtaatga
     6181 tatggaaagt gatatggttt catatcatac aatataatac aatgaccgca tgtcaattat
     6241 ttcgtcgcaa gaaaacaatg gaaaggtcaa actagagatg tgtgcgtaaa aaaaaaaaat
     6301 gtatgtaact aaaaaggtct aaagatgtaa tggtacacac tatacacaat tagacataat
     6361 tacacatgtg gtttagagta ttagatatta ataatgagca gaacaagcta tgtttacagg
     6421 tgcagagaac caagatattc ctttcttttg gttgttgtag atacaaatat attttacaaa
     6481 gctcaatttc aatacaaatg caagaaccac aaacaacagt cctttcaatt tatttggagg
     6541 tggtcttaac tcttaagtat atataaacaa taccacctta taacttttaa gttttaacta
     6601 aaccatactt aaatcttttt taatctctat accaaatctt gcaaatatca aaataacttt
     6661 ttctaaccaa agaaagaaaa catttttgaa gtccttaact tggaaaggaa aagaacaccc
     6721 gggcaaaaaa gattggaaga tacacttcct cccgtaacct ctttaggctt cgtgctaata
     6781 tacgctattt tgacattctc tcctatcata cgcaccttgt attagagatc tcccaaagtc
     6841 tcaccactca catccctttt acgaaatctc agcaagaaaa gattaacatt ttgagaaaag
     6901 tatatattat tgcaacgaaa aaagaagcct cctcctttgt cataaagttc ttaatcaatt
     6961 ttaatttatt gccataatca aaaaacattt tatattttac aaagttcttg aaaaagtagg
     7021 acacttttta aaataatatt cactcatggt acttatttta agctttatta tctgccttat
     7081 aacataagca ttgaaagtac ttgtacattc aactacgttt ctgtgcgtat cagagattac
     7141 gtgtgcttac tctactttat taaatttttg acgtaaactg ttctaaaaca ttaaataata
     7201 atatagatgt atctattgaa ataccaaaac tttattttat aattaatggg aaacaataaa
     7261 atataatatt attcgtttaa agagtctaaa gctgcaacgg catcgccata tacagaaagt
     7321 tttaagcgca ggataagagc atgcacactc tttcccattt ccaagcaaaa taaaacctat
     7381 acatacacat ttatactaat actcatatac attagagaga gaagaagaaa aaatgtggga
     7441 tttttcagag aaataattaa aagaaagcaa aagttttttt tttcttaaaa gcttttgtct
     7501 ctctctcaat tgcttcttgt tcttgtttgc atccggaacc aaagaaagaa actgtgtggt
     7561 ttcatgatgg cacctttggt ttctcttctt cattgaaggg tcgaatttgg tctgagaaaa
     7621 gaagagtgga gagagagaaa gagaagaggc aagagcccca caagagagac atgcttgtct
     7681 gtgtagtccc tattttgtat gttctttaat ctctcccact ctgtttttgt catgtgtaag
     7741 ggttttgtcc ttcgattttg gagtcttctt taaccaaaaa aaaacagatt ccatgaaatg
     7801 gctttgtctt taattcctct ggtttgtcct ggatttatct tcttgtagcc tctttgctca
     7861 atagagctct ttttgtctct tcttgtatta aataaaataa aatgaagact tagtaaagaa
     7921 aaagaaaaac tcttgagtct tgctaaagcc tagttagtgt tgagtgtggg agagacaaaa
     7981 cagaacattg aaaaatctct ctgtatgatg ctcgagctag ttggaatagg ttaacccaac
     8041 tccatactgc agcccggggg atccgatccc cgatcgttca aacatttggc aataaagttt
     8101 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
     8161 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
     8221 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
     8281 ctaggataaa ttatcgcgcg cggtgtcatc tatgtctaga gcggccgcca ccgcggtgga
     8341 gctccagctt ttgttccctt tagtgagggt taattccgag cttggcgtaa tcatggtcat
     8401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8461 gacataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8521 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgaaggcctt gacaggatat
     8761 attggcgggt aaactaagtc gctgtatgtg tttgtttga
//
