LOCUS       40924_35169        7077 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLV14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   De Rybel B, van den Berg W, Lokerse A, Liao CY, van Mourik H, Moller
            B, Peris CL, Weijers D.
  TITLE     A versatile set of ligation-independent cloning vectors for 
            functional studies in plants
  JOURNAL   Plant Physiol. 156 (3), 1292-1299 (2011)
  PUBMED    21562332
REFERENCE   2  (bases 1 to 7077)
  AUTHORS   De Rybel B, van den Berg W, Weijers D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2011) Laboratory of Biochemistry, Wageningen 
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 7077)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7077)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2011"; volume: "156"; issue: "3"; pages: 
            "1292-1299"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2011) Laboratory of Biochemistry, Wageningen University, 
            Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7077
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(66..654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(828..1640)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1931..2366
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    2501..2523
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     polyA_signal    complement(2531..2707)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(2812..3603)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(3629..3974)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     4176..4192
                     /note="M13F; M13 forward primer site"
     primer_bind     4196..4212
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4219..4237
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4263..4279
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4300..4329
                     /note="LIC; ligation-independent cloning (LIC) site"
     intron          4722..4910
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      6351..6599
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(6636..6654)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6675..6691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6699..6715)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6723..6753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6768..6789)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    7028..7052
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
       61 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      121 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      181 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      241 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      301 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      361 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      421 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      481 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      541 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      601 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      661 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
      721 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
      781 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg
      841 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      901 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      961 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1021 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1081 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1141 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     1201 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     1261 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     1321 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     1381 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     1441 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     1501 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     1561 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     1621 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     1681 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     1741 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     1801 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     1861 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     1921 caggcgatcc ccatccaaca gcccgccgtc gagcgggctt ttttatcccc ggaagcctgt
     1981 ggatagaggg tagttatcca cgtgaaaccg ctaatgcccc gcaaagcctt gattcacggg
     2041 gctttccggc ccgctccaaa aactatccac gtgaaatcgc taatcagggt acgtgaaatc
     2101 gctaatcgga gtacgtgaaa tcgctaataa ggtcacgtga aatcgctaat caaaaaggca
     2161 cgtgagaacg ctaatagccc tttcagatca acagcttgca aacacccctc gctccggcaa
     2221 gtagttacag caagtagtat gttcaattag cttttcaatt atgaatatat atatcaatta
     2281 ttggtcgccc ttggcttgtg gacaatgcgc tacgcgcacc ggctccgccc gtggacaacc
     2341 gcaagcggtt gcccaccgtc gagcgccagc gcctttgccc acaacccggc ggccggccgc
     2401 aacagatcgt tttataaatt tttttttttg aaaaagaaaa agcccgaaag gcggcaacct
     2461 ctcgggcttc tggatttccg atccccggaa ttagagatct tggcaggata tattgtggtg
     2521 taacgttatc gatctggatt ttagtactgg attttggttt taggaattag aaattttatt
     2581 gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg
     2641 agcgaaaccc tataagaacc ctaatttccc ttatcgggaa actactcaca cattatttat
     2701 ggagaaaata gagagagata gatttgtaga gagagactgg tgatttcagc gtaccgaatt
     2761 gatcctctag ctagagtccg aaccccagag tcccgcacag agtcccgctc agaagaactc
     2821 gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac
     2881 gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc
     2941 tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg
     3001 gccattttcc accatgatat tcggcaagca ggcatcgccc tgggtcacga cgagatcctc
     3061 gccgtcgggc atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg
     3121 ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac gtcctcgctc
     3181 gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg
     3241 ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag
     3301 atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc
     3361 gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc
     3421 ttggagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg
     3481 cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata
     3541 gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat
     3601 catcttgttc aatcatgcca gcttgggctg tcctctccaa atgaaatgaa cttccttata
     3661 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     3721 tgtcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     3781 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaatgatag
     3841 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttct actgtccttt
     3901 cgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgaa attacccttt
     3961 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgaca tttttggagt
     4021 aggggtacga taacattaac gtttacaatt tcgcgccatt cgccattcag gctgcgcaac
     4081 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     4141 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     4201 acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac cgggcccccc
     4261 ctcgaggtcg acggtatcga taagcttgat atcgaattct agttggaatg ggttaaccca
     4321 actccataag gatcccatgt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
     4381 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
     4441 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
     4501 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
     4561 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
     4621 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
     4681 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtaagtttc tgcttctacc
     4741 tttgatatat atataataat tatcattaat tagtagtaat ataatatttc aaatattttt
     4801 ttcaaaataa aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata
     4861 ttttaattta taacttttct aatatatgac caaaatttgt tgatgtgcag gtatcaccgt
     4921 ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga ttaccgacga
     4981 aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg gaatccatcg
     5041 cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg tggtgacgca
     5101 tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca atggtgatgt
     5161 cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag gcactagcgg
     5221 gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc tctatgaact
     5281 gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg tcggcatccg
     5341 gtcagtggca gtgaagggcg aacagttcct gattaaccac aaaccgttct actttactgg
     5401 ctttggtcgt catgaagatg cggacttgcg tggcaaagga ttcgataacg tgctgatggt
     5461 gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct cgcattaccc
     5521 ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga ttgatgaaac
     5581 tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca acaagccgaa
     5641 agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact tacaggcgat
     5701 taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga gtattgccaa
     5761 cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg cggaagcaac
     5821 gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct gcgacgctca
     5881 caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt acggatggta
     5941 tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac ttctggcctg
     6001 gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata cgttagccgg
     6061 gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat ggctggatat
     6121 gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat ggaatttcgc
     6181 cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag ggatcttcac
     6241 tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga ctggcatgaa
     6301 cttcggtgaa aaaccgcagc agggaggcaa acaatgagga tccgatcccc gatcgttcaa
     6361 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     6421 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     6481 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     6541 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgtctagag
     6601 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattccgagc
     6661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6721 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6781 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6841 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7021 aaggccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttga
//
