LOCUS       40924_35069        4308 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLV01_v2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Wendrich JR, Liao CY, van den Berg WA, De Rybel B, Weijers D.
  TITLE     Ligation-independent cloning for plant research
  JOURNAL   Methods Mol. Biol. 1284, 421-431 (2015)
  PUBMED    25757785
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Wendrich JR, Liao C-Y., van den Berg WAM., De Rybel B, Weijers D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2014) Laboratory of Biochemistry, Wageningen 
            University, Dreijenlaan 3, Wageningen 6703HA, The Netherlands
REFERENCE   3  (bases 1 to 4308)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4308)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Mol. Biol. 1284, 421-431 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-2014) Laboratory of Biochemistry, Wageningen University, 
            Dreijenlaan 3, Wageningen 6703HA, The Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     161..177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        187..205
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     231..247
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    268..297
                     /note="LIC; ligation-independent cloning (LIC) site"
     terminator      311..559
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    581..605
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(696..1284)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1458..2270)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2561..2996
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    3131..3153
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(3164..3416)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3453..4001)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(4043..4222)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
ORIGIN
        1 aacattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
       61 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
      181 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
      241 ggtatcgata agcttgatat cgaattctag ttggaatggg ttaacccaac tccataagga
      301 tccgatcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      361 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      421 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      481 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      541 ggtgtcatct atgtctagag cggccgccac gcgtaggcct tgacaggata tattggcggg
      601 taaactaagt cgctgtatgt gtttgtttga gatctcatgt gagcaaaagg ccagcaaaag
      661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaagaagagt tggtagctct
     1201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1321 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1381 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgtgtaa
     1441 cattggtcta gtgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     1501 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     1561 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     1621 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     1681 ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact
     1741 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     1801 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     1861 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     1921 cctgaatcag gatattcttc taatacctgg aatgctgttt tccctgggat cgcagtggtg
     1981 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     2041 tccgtcagcc agtttagtct gaccatctca tctgtaacaa cattggcaac gctacctttg
     2101 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcggta gattgtcgca
     2161 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     2221 gaatttaatc gcggccttga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     2281 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     2341 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
     2401 gctgagttga aggatcagat cacgcatctt cccgacaacg cagaccgttc cgtggcaaag
     2461 caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc
     2521 ctcactttct ggctggatga tggggcgatt caggcgatcc ccatccaaca gcccgccgtc
     2581 gagcgggctt ttttatcccc ggaagcctgt ggatagaggg tagttatcca cgtgaaaccg
     2641 ctaatgcccc gcaaagcctt gattcacggg gctttccggc ccgctccaaa aactatccac
     2701 gtgaaatcgc taatcagggt acgtgaaatc gctaatcgga gtacgtgaaa tcgctaataa
     2761 ggtcacgtga aatcgctaat caaaaaggca cgtgagaacg ctaatagccc tttcagatca
     2821 acagcttgca aacacccctc gctccggcaa gtagttacag caagtagtat gttcaattag
     2881 cttttcaatt atgaatatat atatcaatta ttggtcgccc ttggcttgtg gacaatgcgc
     2941 tacgcgcacc ggctccgccc gtggacaacc gcaagcggtt gcccaccgtc gagcgccagc
     3001 gcctttgccc acaacccggc ggccggccgc aacagatcgt tttataaatt tttttttttg
     3061 aaaaagaaaa agcccgaaag gcggcaacct ctcgggcttc tggatttccg atccccggaa
     3121 ttagagatct tggcaggata tattgtggtg taacgttatc cccgatctag taacatagat
     3181 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     3241 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     3301 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     3361 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgagc
     3421 ttgcatgctt gcagagatcc gccgggtcat cagatttcgg tgacgggcag gaccggacgg
     3481 ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc
     3541 ttgaagccgg ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc
     3601 acgctcgggt cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc
     3661 agggacttca gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg
     3721 gagacgtaca cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccagggaccc
     3781 gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc
     3841 cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg
     3901 accgtgcttg tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg
     3961 gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cctcgatcga
     4021 gttgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg
     4081 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg
     4141 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctcagt
     4201 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat
     4261 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcgat
//
