LOCUS       40924_35009       10858 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPLEX-501, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Schunmann PHD., Llewellyn DJ, Surin B, Boevink P, De Feyter RC, 
            Waterhouse PM.
  TITLE     A suite of novel promoters and terminators for plant biotechnology
  JOURNAL   Funct. Plant Biol. 30, 443-452 (2003)
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Schunmann PHD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-OCT-2002) Plant Industry, CSIRO, Clunies Ross Street, 
            Canberra, ACT 2602, Australia
REFERENCE   3  (bases 1 to 10858)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10858)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Funct. 
            Plant Biol. 30, 443-452 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-OCT-2002) Plant Industry, CSIRO, Clunies Ross Street, Canberra, 
            ACT 2602, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    168..192
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      672..1385
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(1990..2778)
                     /codon_start=1
                     /product="spectinomycin resistant protein"
                     /label=spectinomycin resistant protein
                     /protein_id="AAP22317.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3875..4463
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5826..6971)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(7246..7596)
                     /label=traJ
                     /note="oriT-recognizing protein"
     mobile_element  complement(7596..8363)
                     /label=IS1
                     /note="prokaryotic transposable element"
     oriT            complement(8423..8532)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_feature    9087..9111
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      9191..9713
                     /note="S1 promoter derived from subterranean clover stunt
                     virus DNA segment 1"
                     /regulatory_class="promoter"
     CDS             9723..10514
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      10699..10837
                     /note="S3 terminator derived from subterranean clover stunt
                     virus DNA segment 3"
                     /regulatory_class="terminator"
ORIGIN
        1 gcggccgcca ccgcggctga gtggctcctt caacgttgcg gttctgtcag ttccaaacgt
       61 aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt gactccctta attctccgct
      121 catgatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat
      181 tggcgggtaa acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg
      241 tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg
      301 gagtgcttgg cattccgtgc gataatgact tctgttcaac cacccaaacg tcggaaagcc
      361 tgacgacgga gcagcattcc aaaaagatcc cttggctcgt ctgggtcggc tagaaggtcg
      421 agtgggctgc tgtggcttga tccctcaacg cggtcgcgga cgtagcgcag cgccgaaaaa
      481 tcctcgatcg caaatccgac gctgtcgaaa agcgtgatct gcttgtcgct ctttcggccg
      541 acgtcctggc cagtcatcac gcgccaaagt tccgtcacag gatgatctgg cgcgagttgc
      601 tggatctcgc cttcaatccg ggtctgtggc gggaactcca cgaaaatatc cgaacgcagc
      661 aagatcgtcg accctttccg acgctcaccg ggctggttgc cctcgccgct gggctggcgg
      721 ccgtctatgg ccctgcaaac gcgccagaaa cgccgtcgaa gccgtgtgcg agacaccgcg
      781 gccggccgcc ggcgttgtgg atacctcgcg gaaaacttgg ccctcactga cagatgaggg
      841 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
      901 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
      961 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     1021 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     1081 cagagtgctg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     1141 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     1201 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctgtgcgcg
     1261 tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc
     1321 gggcctccca tccccccagg ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat
     1381 ggcagccaag ctcctaacat tttattagag agcaggctag ttgcttagat acatgatctt
     1441 caggccgtta tctgtcaggg caagcgaaaa ttggccattt atgacgacca atgccccgca
     1501 gaagctccca tctttgccgc catagacgcc gcgcccccct tttggggtgt agaacatcct
     1561 tttgccagat gtggaaaaga agttcgttgt cccattgttg gcaatgacgt agtagccggc
     1621 gaaagtgcga gacccatttg cgctatatat aagcctacga tttccgttgc gactattgtc
     1681 gtaattggat gaactattat cgtagttgct ctcagagttg tcgtaatttg atggactatt
     1741 gtcgtaattg cttatggagt tgtcgtagtt gcttggagaa atgtcgtagt tggatgggga
     1801 gtagtcatag ggaagacgag cttcatccac taaaacaatt ggcaggtcag caagtgcctg
     1861 ccccgatgcc atcgcaagta cgaggcttag aaccaccttc aacagatcgc gcatagtctt
     1921 ccccagctct ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat
     1981 tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt gaacaaattc
     2041 ttccaactga tctgcgcgcg aggccaagcg atcttcttgt ccaagataag cctgcctagc
     2101 ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt cggcagcgac
     2161 atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca acgtaagcac
     2221 tacatttcgc tcatcgccag cccagtcggg cggcgagttc catagcgtta aggtttcatt
     2281 tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg ccgctggacc
     2341 taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat caatgtcgat
     2401 cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc caaattgcag
     2461 ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc
     2521 tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat
     2581 caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca aatcaatatc
     2641 actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg ccagcaacgt
     2701 cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg atacttcggc
     2761 gatcaccgct tccctcatga tgtttaactc ctgaattaag ccgcgccgcg aagcggtgtc
     2821 ggcttgaatg aattgttagg cgtcatcctg tgctcccgag aaccagtacc agtacatcgc
     2881 tgtttcgttc gagacttgag gtctagtttt atacgtgaac aggtcaatgc cgccgagagt
     2941 aaagccacat tttgcgtaca aattgcaggc aggtacattg ttcgtttgtg tctctaatcg
     3001 tatgccaagg agctgtctgc ttagtgccca ctttttcgca aattcgatga gactgtgcgc
     3061 gactcctttg cctcggtgcg tgtgcgacac aacaatgtgt tcgatagagg ctagatcgtt
     3121 ccatgttgag ttgagttcaa tcttcccgac aagctcttgg tcgatgaatg cgccatagca
     3181 agcagagtct tcatcagagt catcatccga gatgtaatcc ttccggtagg ggctcacact
     3241 tctggtagat agttcaaagc cttggtcgga taggtgcaca tcgaacactt cacgaacaat
     3301 gaaatggttc tcagcatcca atgtttccgc cacctgctca gggatcaccg aaatcttcat
     3361 atgacgccta acgcctggca cagcggatcg caaacctggc gcggcttttg gcacaaaagg
     3421 cgtgacaggt ttgcgaatcc gttgctgcca cttgtttaat agactggatg gaggcggata
     3481 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3541 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3601 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3661 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3721 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3781 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3841 cgtcagaccc cgtagaaaag atcaaaggat cttcttgata tccttttttt ctgcgcgtaa
     3901 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3961 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     4021 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     4081 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     4141 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     4201 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     4261 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     4321 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4381 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4441 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4501 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4561 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4621 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     4681 gcggtatttc acaccgcata tgaagatcgg cggcaatagc ttcttagcgc catcccggct
     4741 gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc ggaaccaaag
     4801 gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa cattggttcc
     4861 tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca gaagtcctcc
     4921 ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc acttgcgggt
     4981 cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga cgccgacagg
     5041 atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc
     5101 ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga tgaacacgac
     5161 catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga
     5221 aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga agatgcgcat
     5281 ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac ccgccggcaa
     5341 cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca cgaaaacgcc
     5401 ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga ccgacagccc
     5461 aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc gttcagcgaa
     5521 cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct ctggagcttt
     5581 cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc caagccgtcg
     5641 aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
     5701 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
     5761 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
     5821 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
     5881 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
     5941 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
     6001 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
     6061 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
     6121 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
     6181 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
     6241 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
     6301 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
     6361 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
     6421 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
     6481 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
     6541 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
     6601 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
     6661 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
     6721 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
     6781 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
     6841 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
     6901 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
     6961 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
     7021 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
     7081 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
     7141 tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg
     7201 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg
     7261 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag
     7321 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag
     7381 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg
     7441 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga
     7501 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
     7561 gtacaccttg ataggtgggc tgcccttcct ggttgggtaa tgactccaac ttattgatag
     7621 tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac
     7681 gacagcgact tccgtcccag ccgtgccagg tgctgcctca gattcaggtt atgccgctca
     7741 attcgctgcg tatatcgctt gctgattacg tgcagctttc ccttcaggcg ggattcatac
     7801 agcggccagc catccgtcat ccatatcacc acgtcaaagg gtgacagcag gctcataaga
     7861 cgccccagcg tcgccatagt gcgttcaccg aatacgtgcg caacaaccgt cttccggaga
     7921 ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacata gccccactgt
     7981 tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga ggttaccgac
     8041 tgcggcctga gttttttaag tgacgtaaaa tcgtgttgag gccaacgccc ataatgcggg
     8101 ctgttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg tgcgtaccgg
     8161 gttgagaagc ggtgtaagtg aactgcagtt gccatgtttt acggcagtga gagcagagat
     8221 agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg
     8281 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
     8341 atcagtaagt tggcagcatc acccctggtt ggcttggttt catcagccat ccgcttgccc
     8401 tcatctgtta cgccggcggt agccggccag cctcgcagag caggattccc gttgagcacc
     8461 gccaggtgcg aataagggac agtgaagaag gaacacccgc tcgcgggtgg gcctacttca
     8521 cctatcctgc ccggctgacg ccgttggata caccaaggaa agtctacacg aaccctttgg
     8581 caaaatcctg tatatcgtgc gaaaaaggat ggatataccg aaaaaatcgc tataatgacc
     8641 ccgaagcagg gttatgcagc ggaaaagatc cgtcgacgat cgtcaacgtt cacttctaaa
     8701 gaaatagcgc cactcagctt cctcagcggc tttatccagc gatttcctat tatgtcggca
     8761 tagttctcaa gatcgacagc ctgtcacggt taagcgagaa atgaataaga aggctgataa
     8821 ttcggatctc tgcgagggag atgatatttg atcacaggca gcaacgctct gtcatcgtta
     8881 caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc
     8941 gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg
     9001 ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc
     9061 cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg
     9121 cttagacaac ttaataacac attgcggacg tttttaatgt actgaattct agggccgctc
     9181 tagaggatcc tatgttgtaa ttttatatgg attaatgaga attattatta ttctgttctt
     9241 cgtctgtgtt ttttaagcta gcttcctcga agcagcgtat aactttaatt tgaatttggt
     9301 tttggcgcgt tagtgaaatt gcggctgtaa acgtgtcaag ttgtgagtgg ctgaaataag
     9361 ataatagata tattattatt gttttaattt aattccgcga agcgatatgt taagtgataa
     9421 atgaaacgaa gcgttttgat gacgtcatat gtctccgtgc ctacgtcagc acggggctta
     9481 gtattacccc cgtgccggga tcagagacat ttgaccaata gttgactagt ataatagccc
     9541 ttggattaaa tgacacgtgg acgctcagga tctgtgatgc tagtgaagcg cttaagctga
     9601 acgaatctga cggaagagcg ttcacactta gatctagtta gcgtacttag tacgcgttgt
     9661 cttgggtcta taaatagagt gcttctgaac agattgttca gaatttcata gcgccgataa
     9721 caatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     9781 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     9841 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     9901 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     9961 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
    10021 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
    10081 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
    10141 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
    10201 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
    10261 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
    10321 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
    10381 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
    10441 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
    10501 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
    10561 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    10621 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    10681 cgcccacccc gggtcgacgg tattgagttt atgtctattg taattgatag aggttctatt
    10741 aagatagaat tatgagatgt aattgtgatt aatgaataaa gagttgttat tattctttga
    10801 attactccgc gaagcggtgt gttatgtttt tgttggaagc tttctagact cgactaga
//
