LOCUS       V004059                 9015 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004059
VERSION     V004059
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9015)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9015)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9015)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9015)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     regulatory      1273..1282
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1282..1311
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    1312..2031
                     /label="HO1 PCC7002 optimized"
                     /note="HO1 PCC7002 optimized"
     CDS             2041..2097
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             2101..2124
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2131..2865
                     /label="PcyA"
                     /note="PcyA"
     promoter        2900..3229
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3239..3835
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4131..4364
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4521..4745
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4788..4797
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4841..4945
                     /label="AmpR promoter"
     CDS             4946..5803
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5977..6565
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             6724..7357
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7404..7529
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8022..8255
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8440..8484
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8633..8674
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    8771..8888
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9015
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgctagc
     1261 acattcgaag ccgccaccat ggaacagaag ctgatctccg aagaggacct gatggtcgcc
     1321 aacctggcca ccatgctgag ggagggcacc aagacaagtc acaccatggc cgagaatgtc
     1381 ggattcgtga aatgctttct caagggtgtt gtggaaaaga agtcctacag gaagctggtg
     1441 gctgacctgt attacgtgta ctcagccatg gaagaggaga tggagagact gaaagatcat
     1501 cctgtcgtgt cccagatcta ttttccagaa ctcaacagga agcagtcact ggagacagac
     1561 ctgagatatt acttcggacc caattggcag gccgaggcca aaattacccc cgcaggacag
     1621 gcctatgtgg acaggattca tgaggtggct cagaccgccc ctgaactcct ggtctcacac
     1681 tcctataccc gctatctcgg cgacctgagc ggaggccaga tcctgaagaa gatcgcacag
     1741 aacgcaatga atctggacgg ggagggaacc gctttctacg agtttgagaa tatcagtgac
     1801 gagaaagcct tcaaagataa gtaccgggca gcaatgaata gcctggatgt gccagaggaa
     1861 actgctgaac agatcgtgca ggaagccaat gatgccttcg gcatgaacat gaatatgttt
     1921 aaggagctgg aaggtaatct ggtcaaggcc atcggcgtca tgctgtttaa taccctgaca
     1981 cgccggagaa ccaagggcag cacagacgca gacctgtcac ctgcttctaa tggaagcgga
     2041 gctactaact tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctatg
     2101 gattacaagg atgacgacga taagtctagc atgactgctc ctgccacaaa acctaagttt
     2161 taccctctga ttgagcagct ggcaggagtg atcctggaga cctggcacca gcatctggag
     2221 ctggaaccct ttaccctgcc tgaaggactg ggctatattg aaggcaagct ggaaggcgag
     2281 agactgacta ttgagaacac ctgttaccag agccagcact tcaggaagat gcacctggag
     2341 atggccaaag tgggagaaaa cctggacatc ctgcactgcg tcatgtttcc cagggccgaa
     2401 tatgctctgc ccatgttcgg ctgtgacctg gtcggcaatc caagaggcgt gagtgccgct
     2461 attgcagacc tgtctcctac ctccgcagac aagactctgt ccgtggacta tcagacaagg
     2521 ctgagacaga accaggggaa tctgaacttc agtcagccaa gagacctgcc cgaatgggga
     2581 gcaatcttca gcgagttttg cctgtttatc cggccaagta atcccaacga agagcagcag
     2641 tttctggagc gcgtgagaga gttcctgaca atccattgtc agctggccct gggcctggaa
     2701 aagctgagcc cagcagaaca ggctatctat ctggctggcc agaggcacta ttgccaggag
     2761 cagcagagga atgacaaaac caggagagtg ctggagaaag cctttaaccc cgattgggca
     2821 gagcgataca tgagccaggt gctgtttgac atccccgcct aataagaatt cgccagcaca
     2881 gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     3001 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     3061 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3121 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3181 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat
     3241 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     3301 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     3361 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     3421 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     3481 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     3541 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     3601 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     3661 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     3721 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     3781 cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc cgcaagcccg gtgcctgacg
     3841 cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgataaa
     3901 ataaaagatt ttatttagtc tccagaaaaa gggggcggcc gctcgagacc tagaaaaaca
     3961 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     4021 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     4081 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4141 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4201 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4261 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4321 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     4381 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     4441 acattataat agatttagca ggaattgaac taggagtgga gcacacaggc aaagttctag
     4501 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4561 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4621 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4681 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4741 tatggcttct gaggcggaaa gaaccagctg ggggcgcgcc cctcgaggcc gccatggtca
     4801 tagctgtttg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4861 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4921 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4981 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5041 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5101 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5161 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5221 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5281 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5341 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5401 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5461 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5521 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5581 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5641 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5701 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5761 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5821 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5881 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5941 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6001 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6061 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6121 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6181 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6241 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6301 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6361 gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6421 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6481 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6541 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6601 cttttgccgg accgccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     6661 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6721 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     6781 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     6841 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     6901 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     6961 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7021 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7081 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7141 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7201 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7261 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7321 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7381 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7441 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7501 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7561 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7621 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7681 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7741 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7801 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     7861 agcaaccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     7921 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     7981 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     8041 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     8101 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     8161 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8221 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8281 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8341 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8401 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8461 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8521 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8581 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8641 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8701 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccacaa atggcagtat
     8761 tcatccacaa ttttaaaaga aaggggggga ttggggggta cagtgcaggg gaaagaatag
     8821 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     8881 aaaattttcg ggtttattac agggacagca gagatccact ttggtcgata agctttgcaa
     8941 agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt ctaggtcttg
     9001 aaaggagtgg gaatt
//
