LOCUS       V004060                 9330 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004060
VERSION     V004060
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9330)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9330)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9330)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9330)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1279..1353
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     regulatory      1375..1384
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1384..1413
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    1414..2133
                     /label="sHO1"
                     /note="sHO1"
     misc_feature    1689..2133
                     /label="HO1"
                     /note="HO1"
     CDS             2143..2199
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     misc_feature    2200..2286
                     /label="mito"
                     /note="mito"
     CDS             2200..2274
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2302..2325
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2332..3192
                     /label="Hy2 hyper"
                     /note="Hy2 hyper"
     promoter        3215..3544
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3554..4150
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4446..4679
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4836..5060
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      5103..5112
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5156..5260
                     /label="AmpR promoter"
     CDS             5261..6118
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6292..6880
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             7039..7672
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7719..7844
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8337..8570
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8755..8799
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8948..8989
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    9086..9203
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9330
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgctagc
     1261 acattcgaag ccgccaccat gtccgtcttg acaccattgt tgttgagagg gttgacaggt
     1321 tcagccagga gattgccagt gcccagagcc aaaatacatt cattattcga agccgccacc
     1381 atggaacaga agctgatctc cgaagaggac ctgatggtcg ccaacctggc caccatgctg
     1441 agggagggca ccaagacaag tcacaccatg gccgagaatg tcggattcgt gaaatgcttt
     1501 ctcaagggtg ttgtggaaaa gaagtcctac aggaagctgg tggctgacct gtattacgtg
     1561 tactcagcca tggaagagga gatggagaga ctgaaagatc atcctgtcgt gtcccagatc
     1621 tattttccag aactcaacag gaagcagtca ctggagacag acctgagata ttacttcgga
     1681 cccaattggc aggcagaggc aaagattacc cccgcaggac aggcttacgt cgataggatt
     1741 cacgaagtgg cacagaccgc accagagctg ctggtgtcac acagctacac tagatacctg
     1801 ggcgacctgt ctggcggaca gatcctgaag aaaattgcac agaacgccat gaatctggat
     1861 ggggagggca ccgctttcta cgagtttgaa aacatctctg acgaaaaggc attcaaggat
     1921 aaatatcgcg ccgctatgaa tagtctggac gtgcctgagg aaaccgccga gcagatcgtc
     1981 caggaagcta acgatgcatt cggaatgaac atgaatatgt ttaaggagct ggaaggcaac
     2041 ctggtgaaag ctattggagt catgctgttc aataccctga caaggagacg gactaagggg
     2101 agcaccgacg cagatctgag cccagcctcc aacggatctg gggccacaaa tttttccctg
     2161 ctgaaacagg ctggggacgt ggaggaaaat ccaggcccca tgtctgtcct gaccccactg
     2221 ctgctgcgag gactgacagg aagtgcccgc cgactgcctg tgccacgcgc taagatccac
     2281 tcactggcat ccggatctat ggattacaag gacgatgacg ataagagcag catgctccga
     2341 gtaagtgcgg tcagctataa ggagttcgct gaaagtgctc tggaggaaac aaggaaaaga
     2401 atcgtgctgg agccatcaca cctccaggaa aagtatagct caatgacagg cctggatggg
     2461 aagacggaac tccagatgtt ggccttcaag agctcaaaaa tccgcctcct tagatcaatg
     2521 gcaatcgaga acgaaacaat gcaggttttt gatttcgcag gtttcatgga gcctgaatat
     2581 gacacgccaa ttttctgcgc caatttcttc actagcacta atgttaatat tgttgtcctg
     2641 gacctgaatc ccctccatca gctgacagac cagaccgact atcaggacaa atactacaac
     2701 aaaatcatga gcatctatca taagtacgct gagacattcc cttggggggg caaactgacc
     2761 ggagagtcta tcaagttttt ttctccactg gtgatgtgga cacgatttag ttccagtaag
     2821 gagaagcata aagccctttt cagcgccttc ctggaatatt accaggcttg gctggagatg
     2881 acaatacagg tgcgagaaga gatggaaccc tcacatgtca gggctaattg cgaggcacaa
     2941 cacaaatatc tgacctggag agcccagaag gaccctggcc atggccttct caagaggctg
     3001 gtgggagagg ccaaggccaa ggaactgctg agggacttcc tgtttaacgg cgtggatgag
     3061 ttgggtacaa aaacctttat tgactacttc cccgaatacc aaaccgaaga tgggaccgtc
     3121 agtgacaaga ggagtataat cgggaaaagc tacgaaactc ggccatggga cctgaccgga
     3181 caatttattg ggtagtgagt cgaccctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3361 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3481 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3541 caaaaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3601 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3661 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3721 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3781 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3841 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3901 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3961 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4021 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4081 caccgtcacc gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa
     4141 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     4201 ccatgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg cggccgctcg
     4261 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     4321 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     4381 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4441 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4501 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4561 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4621 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4681 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4741 gagtgagagg ccttgacatt ataatagatt tagcaggaat tgaactagga gtggagcaca
     4801 caggcaaagt tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4861 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4921 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     4981 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5041 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctgggggc gcgcccctcg
     5101 aggccgccat ggtcatagct gtttgacgtc aggtggcact tttcggggaa atgtgcgcgg
     5161 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5221 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5281 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5341 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5401 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5461 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5521 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5581 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5641 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5701 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5761 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5821 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5881 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5941 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6001 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6061 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6121 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6181 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6241 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6301 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6361 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6421 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6481 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6541 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6601 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6661 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     6721 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6781 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6841 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6901 tttacggttc ctggcctttt gccggaccgc cactgccaat tacctgtggt ttcatttact
     6961 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     7021 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     7081 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     7141 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     7201 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     7261 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     7321 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     7381 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     7441 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     7501 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7561 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7621 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7681 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7741 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7801 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7861 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7921 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7981 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     8041 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     8101 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     8161 gtaagaccac cgcacagcaa ccggccgctg atcttcagac ctggaggagg agatatgagg
     8221 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     8281 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     8341 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     8401 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     8461 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     8521 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     8581 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     8641 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     8701 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     8761 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     8821 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     8881 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     8941 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     9001 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc
     9061 cacaaatggc agtattcatc cacaatttta aaagaaaggg ggggattggg gggtacagtg
     9121 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     9181 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggt
     9241 cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
     9301 accttctagg tcttgaaagg agtgggaatt
//
