LOCUS       V004062                 9216 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004062
VERSION     V004062
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9216)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9216)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9216)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9216)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1279..1353
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     regulatory      1375..1384
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1384..1413
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    1414..2133
                     /label="HO1 PCC7002 optimized"
                     /note="HO1 PCC7002 optimized"
     CDS             2143..2199
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     misc_feature    2200..2286
                     /label="transit peptide Mito"
                     /note="transit peptide Mito"
     CDS             2200..2274
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2302..2325
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2332..3066
                     /label="PcyA"
                     /note="PcyA"
     promoter        3101..3430
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3440..4036
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4332..4565
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4722..4946
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4989..4998
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5042..5146
                     /label="AmpR promoter"
     CDS             5147..6004
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6178..6766
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             6925..7558
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7605..7730
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8223..8456
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8641..8685
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8834..8875
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    8972..9089
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9216
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgctagc
     1261 acattcgaag ccgccaccat gtccgtcttg acaccattgt tgttgagagg gttgacaggt
     1321 tcagccagga gattgccagt gcccagagcc aaaatacatt cattattcga agccgccacc
     1381 atggaacaga agctgatctc cgaagaggac ctgatggtcg ccaacctggc caccatgctg
     1441 agggagggca ccaagacaag tcacaccatg gccgagaatg tcggattcgt gaaatgcttt
     1501 ctcaagggtg ttgtggaaaa gaagtcctac aggaagctgg tggctgacct gtattacgtg
     1561 tactcagcca tggaagagga gatggagaga ctgaaagatc atcctgtcgt gtcccagatc
     1621 tattttccag aactcaacag gaagcagtca ctggagacag acctgagata ttacttcgga
     1681 cccaattggc aggccgaggc caaaattacc cccgcaggac aggcctatgt ggacaggatt
     1741 catgaggtgg ctcagaccgc ccctgaactc ctggtctcac actcctatac ccgctatctc
     1801 ggcgacctga gcggaggcca gatcctgaag aagatcgcac agaacgcaat gaatctggac
     1861 ggggagggaa ccgctttcta cgagtttgag aatatcagtg acgagaaagc cttcaaagat
     1921 aagtaccggg cagcaatgaa tagcctggat gtgccagagg aaactgctga acagatcgtg
     1981 caggaagcca atgatgcctt cggcatgaac atgaatatgt ttaaggagct ggaaggtaat
     2041 ctggtcaagg ccatcggcgt catgctgttt aataccctga cacgccggag aaccaagggc
     2101 agcacagacg cagacctgtc acctgcttct aatggaagcg gagctactaa cttcagcctg
     2161 ctgaagcagg ctggagacgt ggaggagaac cctggaccta tgagtgttct gaccccattg
     2221 ctgttgagag gactgactgg ttccgccagg aggttgcccg tgccaagagc caaaatacat
     2281 agcttagcta gcggatccat ggattacaag gatgacgacg ataagtctag catgactgct
     2341 cctgccacaa aacctaagtt ttaccctctg attgagcagc tggcaggagt gatcctggag
     2401 acctggcacc agcatctgga gctggaaccc tttaccctgc ctgaaggact gggctatatt
     2461 gaaggcaagc tggaaggcga gagactgact attgagaaca cctgttacca gagccagcac
     2521 ttcaggaaga tgcacctgga gatggccaaa gtgggagaaa acctggacat cctgcactgc
     2581 gtcatgtttc ccagggccga atatgctctg cccatgttcg gctgtgacct ggtcggcaat
     2641 ccaagaggcg tgagtgccgc tattgcagac ctgtctccta cctccgcaga caagactctg
     2701 tccgtggact atcagacaag gctgagacag aaccagggga atctgaactt cagtcagcca
     2761 agagacctgc ccgaatgggg agcaatcttc agcgagtttt gcctgtttat ccggccaagt
     2821 aatcccaacg aagagcagca gtttctggag cgcgtgagag agttcctgac aatccattgt
     2881 cagctggccc tgggcctgga aaagctgagc ccagcagaac aggctatcta tctggctggc
     2941 cagaggcact attgccagga gcagcagagg aatgacaaaa ccaggagagt gctggagaaa
     3001 gcctttaacc ccgattgggc agagcgatac atgagccagg tgctgtttga catccccgcc
     3061 taataagaat tcgccagcac agtggtcgac cctgtggaat gtgtgtcagt tagggtgtgg
     3121 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3181 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3241 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     3301 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     3361 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     3421 cttttgcaaa aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     3481 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     3541 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     3601 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     3661 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     3721 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     3781 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     3841 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     3901 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     3961 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac
     4021 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     4081 acgaccccat gcatcgataa aataaaagat tttatttagt ctccagaaaa agggggcggc
     4141 cgctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4201 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     4261 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     4321 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     4381 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4441 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4501 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4561 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4621 atcagagagt gagaggcctt gacattataa tagatttagc aggaattgaa ctaggagtgg
     4681 agcacacagg caaagttcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     4741 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4801 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4861 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4921 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggggcgcgc
     4981 ccctcgaggc cgccatggtc atagctgttt gacgtcaggt ggcacttttc ggggaaatgt
     5041 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5101 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5161 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5221 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5281 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5341 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5401 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5461 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5521 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     5581 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     5641 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     5701 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     5761 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     5821 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     5881 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     5941 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6001 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6061 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6121 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6181 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6241 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6301 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6361 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6421 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6541 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     6601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     6661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     6721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     6781 ggccttttta cggttcctgg ccttttgccg gaccgccact gccaattacc tgtggtttca
     6841 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     6901 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     6961 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     7021 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     7081 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     7141 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     7201 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     7261 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     7321 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     7381 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7441 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7501 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7561 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7621 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7681 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7741 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7801 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7861 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7921 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7981 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8041 acaaaagtaa gaccaccgca cagcaaccgg ccgctgatct tcagacctgg aggaggagat
     8101 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8161 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8221 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8281 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8341 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8401 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8461 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8521 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8581 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8641 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8701 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8761 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8821 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8881 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     8941 tatcgccaca aatggcagta ttcatccaca attttaaaag aaaggggggg attggggggt
     9001 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     9061 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     9121 tttggtcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     9181 taatggacct tctaggtctt gaaaggagtg ggaatt
//
