LOCUS       V004063                 9267 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004063
VERSION     V004063
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9267)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9267)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9267)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9267)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1279..1353
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     regulatory      1375..1384
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1384..1425
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             1432..1722
                     /gene="petF"
                     /label="Ferredoxin-1"
                     /note="Ferredoxin-1 from Synechococcus sp. (strain ATCC
                     27264 / PCC 7002 / PR-6). Accession#: P31965"
     CDS             1738..1794
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     misc_feature    1795..1881
                     /label="transit peptide mito(1)"
                     /note="transit peptide mito(1)"
     CDS             1795..1869
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1900..1917
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             1924..3129
                     /gene="petH"
                     /label="Ferredoxin--NADP reductase"
                     /note="Ferredoxin--NADP reductase from Synechococcus sp.
                     (strain ATCC 27264 / PCC 7002 / PR-6). Accession#: P31973"
     promoter        3152..3481
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3491..4087
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4383..4616
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4773..4997
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      5040..5049
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5093..5197
                     /label="AmpR promoter"
     CDS             5198..6055
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6229..6817
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             6976..7609
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7656..7781
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8274..8507
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8692..8736
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8885..8926
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    9023..9140
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9267
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgctagc
     1261 acattcgaag ccgccaccat gtccgtcttg acaccattgt tgttgagagg gttgacaggt
     1321 tcagccagga gattgccagt gcccagagcc aaaatacatt cattattcga agccgccacc
     1381 atgggcaagc ccatccccaa ccccctgctg ggcctggact ccaccgttaa catggctact
     1441 tacaaagtga ccctgattac acccgatggg gaggtgtcct acgatgcccc cgacgatgag
     1501 tatattctgg acagcgccgg cgatgcaggg tacgatctgc cagcttcttg cagagctggc
     1561 gcctgcagta cctgcgcagg gaaaatcgtg agcggaacag tggatcagag tgaacagtcc
     1621 ttcctggacg atgaccagat cgaggccggc tatgtgctga catgtattgc ctacccacag
     1681 tccgatgtga ccattgagac caacaaggag gaagagctgt acgtgtacgg aagcggagct
     1741 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatgtcc
     1801 gtcttgacac cattgttgtt gagagggttg acaggttcag ccaggagatt gccagtgccc
     1861 agagccaaaa tacattcatt agaattcgcc gccaccatgc atcatcacca ccaccatgtt
     1921 aacatgtatg gaatcacctc aaccgccaac tcaaccggaa atcagtccta cgctaatcgg
     1981 ctgttcatct acgaagtggt cgggctggga ggcgacggac ggaacgagaa tagcctggtg
     2041 cgcaagtccg ggaccacatt catcactgtc ccttacgcac gaatgaacca ggaaatgcag
     2101 aggattacca agctgggcgg aaaaatcgtg tctattcgac cagccgagga tgccgctcag
     2161 atcgtcagtg aaggacagag ctccgctcag gcatcagccc agagccctat ggccagcagc
     2221 acaaagattg tgcaccccaa aactaccgac actagcgtgc ctgtcaacat ctaccgccct
     2281 aagaccccat tcctgggcaa atgcattgag aattatgaac tggtggatga ggggggctca
     2341 ggaactgtga ggcatgtcac ctttgacatc agcgaggggg atctgagata cctggaaggg
     2401 cagtccattg gcatcattcc ccctggagag gacaaaaacg ggaagcctca caaactgcgc
     2461 ctgtattcca tcgcctctac ccgacatggc gacatggagg ataataagac cgtgagcctg
     2521 tgcgtgaggc agctggaata ccaggacccc gagtctggcg aaaccgtgta cggcgtgtgc
     2581 agcacatacc tgtgcaacct gccagtggga acagacgatg tcaagatcac tggaccagtg
     2641 gggaaagaga tgctgctgcc cgacgatgaa gacgcaacag tggtcatgct ggccacaggg
     2701 actggcattg ccccattcag ggcttttctg tggagaatgt ttaaggagca gcacgaagat
     2761 tataagttca aagggaaggc ctggctgatc tttggcgtgc cctacactgc taatattctg
     2821 tataaggacg atttcgagaa aatggcagcc gaaaaccctg acaacttcag gctgacttac
     2881 gctatctccc gggagcagaa gaccgcagac ggagggaaag tgtacgtcca gagtcgggtg
     2941 tcagaatatg ccgatgagct gtttgaaatg atccagaagc ccaacacaca cgtgtacatg
     3001 tgcggcctga aaggaatgca gccacccatt gacgagacct ttacagctga ggcagaaaag
     3061 cgcggcctga attgggagga aatgaggagg tctatgaaaa aagagcatcg gtggcatgtg
     3121 gaggtgtatt gataagtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3181 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3241 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3301 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3361 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3421 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3481 aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     3541 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     3601 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     3661 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     3721 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     3781 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     3841 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     3901 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     3961 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     4021 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc
     4081 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     4141 tgcatcgata aaataaaaga ttttatttag tctccagaaa aagggggcgg ccgctcgaga
     4201 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     4261 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     4321 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4381 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     4441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     4501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     4561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     4621 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     4681 tgagaggcct tgacattata atagatttag caggaattga actaggagtg gagcacacag
     4741 gcaaagttct agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     4801 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     4861 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     4921 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     4981 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tgggggcgcg cccctcgagg
     5041 ccgccatggt catagctgtt tgacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5341 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6361 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6601 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6841 acggttcctg gccttttgcc ggaccgccac tgccaattac ctgtggtttc atttactcta
     6901 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     6961 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     7021 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     7081 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     7141 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     7201 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     7261 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     7321 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     7381 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     7441 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     7501 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     7561 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     7621 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     7681 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     7741 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     7801 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     7861 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     7921 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     7981 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     8041 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     8101 agaccaccgc acagcaaccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     8161 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     8221 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     8281 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     8341 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     8401 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     8461 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     8521 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     8581 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     8641 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     8701 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     8761 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     8821 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     8881 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     8941 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccac
     9001 aaatggcagt attcatccac aattttaaaa gaaagggggg gattgggggg tacagtgcag
     9061 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9121 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggtcga
     9181 taagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
     9241 ttctaggtct tgaaaggagt gggaatt
//
