LOCUS       V004064                 9204 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004064
VERSION     V004064
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9204)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9204)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9204)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1264..1338
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     regulatory      1360..1369
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..1398
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     misc_feature    1399..2127
                     /label="tHO1"
                     /note="tHO1"
     CDS             2137..2193
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             2194..2268
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             2296..2319
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    2326..3051
                     /label="tPCYA"
                     /note="tPCYA"
     regulatory      2760..2769
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        3089..3418
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3428..4024
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4320..4553
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4710..4934
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4977..4986
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5030..5134
                     /label="AmpR promoter"
     CDS             5135..5992
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6166..6754
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             6913..7546
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7593..7718
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8211..8444
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8629..8673
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8822..8863
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    8960..9077
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9204
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgccgcc
     1261 accatgtccg tgctgacccc actcctgctt aggggcctta ccggctctgc caggcgcctc
     1321 ccggtcccac gggcaaagat ccactcattg ttcgaagccg ccaccatgga acagaagctg
     1381 atctccgaag aggacctgat gggcacagcc accatgacaa ccagtttggc tacaaagttg
     1441 agagagggta ctaaaaaggc tcacaccatg gcagagaacg tggggttcgt gcggtgtttt
     1501 ctgaagggga cagttgagaa aagctcttat aggaaactgg tcgcgtcctt gtaccatgtc
     1561 tattccgcga tggagcaaga gatggagaga ttgaaggatc acccaatcgt tgggaagatc
     1621 tattttcctg agctgaatcg gaagagtagt ctggagaggg atttgaccta ttactttggt
     1681 agcaattggc gcgaagagat cccaccctct ccagccactc aggcttacgt ggcccgaatc
     1741 catgaggtgg caaacactgc ccccgaactg ctggtagcac attcatatac tcgatatctt
     1801 ggggatctct ccggaggtca gattcttaaa ggcatcgctg agcgagcaat gaatctgcaa
     1861 gatggagagg gcactgcttt ctatcggttt gagagcatct ctgacgagaa agcctttaaa
     1921 cagctgtatc gacagcggct cgacgaactg cctgtcgacg aggccaccgc agaccgcatc
     1981 gtcgacgagg ctaatgctgc tttcggaatg aacatgaaga tctttcagga gctggaaggg
     2041 aatctgatcc gggctatcgg tcaactgctc tttaacactc tgaccaggcg gaagcagagg
     2101 gggtctactg agcttgctac agccgatgga tctggggcca caaatttttc cctgctgaaa
     2161 caggctgggg acgtggagga aaatccaggc cccatgagcg ttctcacacc tttgctgctc
     2221 cgggggctca caggcagtgc tcgacgactg ccagtcccga gggcgaagat tcactcactg
     2281 gctagcggat caatggatta caaggatgac gacgataagt ctagcatggg gactgcaact
     2341 atgtctctga ggcaacatca acatcctctc atccagagac tcgcagatag aattgaggca
     2401 atttggcagg cctttttccc actggctcca tacgcactgc cggaggactt gggctacgtg
     2461 gaagggaagc ttgaaggcga gaggttgact attgaaaatc actgttatca ggccccgcct
     2521 tttagaaaac tgcatttgga gttggcgagg gtcggtgagt ctctggatat tttgcactgt
     2581 gtcatgtttc ccgaacctcg gtacgacctt cccatgttcg gctgcgatct ggtgggaggc
     2641 cggggccaaa tctctgccgc aattgttgac ctctcaccag tgaccggaca gttgccagct
     2701 gcctacacct gtgctttgaa cgcactgcct aagctgacat ttagacagcc ccgagagctg
     2761 ccaccatggg gacacatttt ttcacccttc tgcattttta ttaggcccca gggcgaggct
     2821 gaggagcagc agtttttgga tagaatcggt gaatatctta ctctgcactg ccagttgtcc
     2881 caacaggctg tgccaaccga ccaccctcaa gcagtcattg ctggccagcg gcagtattgt
     2941 cagcagcagc agcaaaacga taagacaagg agggtactgg agaaggcatt tggtgtaccc
     3001 tgggccgagc ggtatatgac tactgttctg ttcgacgttc cacccgtgta gtaagaattc
     3061 gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     3121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     3181 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     3241 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3301 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3361 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     3421 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     3481 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     3541 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     3601 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     3661 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     3721 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     3781 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     3841 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     3901 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     3961 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg
     4021 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     4081 atcgataaaa taaaagattt tatttagtct ccagaaaaag ggggcggccg ctcgagacct
     4141 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     4201 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     4261 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4321 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4381 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4441 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4501 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     4561 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     4621 gaggccttga cattataata gatttagcag gaattgaact aggagtggag cacacaggca
     4681 aagttctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4741 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4801 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4861 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4921 ggtgggctct atggcttctg aggcggaaag aaccagctgg gggcgcgccc ctcgaggccg
     4981 ccatggtcat agctgtttga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     5341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6301 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6541 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6781 gttcctggcc ttttgccgga ccgccactgc caattacctg tggtttcatt tactctaaac
     6841 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     6901 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     6961 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     7021 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     7081 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     7141 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     7201 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     7261 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     7321 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     7381 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7441 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7501 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     7561 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     7621 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     7681 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7741 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7801 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7861 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7921 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7981 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     8041 ccaccgcaca gcaaccggcc gctgatcttc agacctggag gaggagatat gagggacaat
     8101 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     8161 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     8221 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     8281 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     8341 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     8401 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     8461 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     8521 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     8581 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     8641 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     8701 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8761 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8821 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     8881 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccacaaa
     8941 tggcagtatt catccacaat tttaaaagaa agggggggat tggggggtac agtgcagggg
     9001 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     9061 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggtcgataa
     9121 gctttgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc
     9181 taggtcttga aaggagtggg aatt
//
