LOCUS       V004065                 9201 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004065
VERSION     V004065
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Kyriakakis P, Catanho M, Hoffner N, Thavarajah W, Jian-Yu V, Chao
            SS, Hsu A, Pham V, Naghavian L, Dozier LE, Patrick G, Coleman T.
  TITLE     Biosynthesis of Orthogonal Molecules Using Ferredoxin and
            Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB
            Optogenetics
  JOURNAL   ACS Synth Biol (2018) In press
   PUBMED   29301067
REFERENCE   2  (bases 1 to 9201)
  AUTHORS   Kyriakakis P.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive,
            PFBH room 251 MC0412, La Jolla, CA 92093, United States of America
REFERENCE   3  (bases 1 to 9201)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9201)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
            Biol (2018) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-NOV-2017) Bioengineering, UCSD, 9500 Gilman drive, PFBH room 251
            MC0412, La Jolla, CA 92093, United States of America"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             1264..1338
                     /label="COX8 presequence"
                     /note="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
     regulatory      1360..1369
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1369..1410
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             1417..1710
                     /gene="petF1"
                     /label="Ferredoxin-1"
                     /note="Ferredoxin-1 from Synechococcus elongatus.
                     Accession#: P0A3D0"
     CDS             1720..1776
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     misc_feature    1777..1863
                     /label="transit peptide mito(1)"
                     /note="transit peptide mito(1)"
     CDS             1777..1851
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label="COX8 presequence"
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             1882..1899
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_feature    1906..3063
                     /label="FNR THEEB"
                     /note="FNR THEEB"
     promoter        3086..3415
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3425..4021
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             4317..4550
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4707..4931
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      4974..4983
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5027..5131
                     /label="AmpR promoter"
     CDS             5132..5989
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6163..6751
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     LTR             6910..7543
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7590..7715
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8208..8441
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8626..8670
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             8819..8860
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    8957..9074
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        9201
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattgatcc ttcgaactag
     1201 aattcgaagc ttgagctcga gatctgagtc cggtagcgct agttcgaagg atccgccgcc
     1261 accatgagcg tgctgacgcc cttgctgctt agaggactga ccggaagcgc acgccgcctg
     1321 cccgtgccta gggcgaagat acatagcctg ttcgaagccg ccaccatggg taagcccatt
     1381 cccaacccgc tgttgggcct cgatagcaca gttaacatgg ctacctacaa agtgacactg
     1441 gttcggcctg atggtagcga gacaacaatt gacgtgcctg aggacgaata catactcgac
     1501 gttgcggaag agcaagggct ggatctgcca tttagttgcc gagcgggcgc ttgcagcact
     1561 tgtgccggga agttgctcga aggggaagtg gatcagtctg atcagtcctt tctggatgac
     1621 gatcagatcg aaaaagggtt cgtgctcacc tgtgtcgctt atccaaggag cgattgtaag
     1681 attctcacta atcaggagga ggagctttac ggctcaggcg cgacaaattt ttctctcctc
     1741 aaacaggctg gggacgtgga ggagaaccct ggccctatgt ccgttcttac cccactgttg
     1801 ttgcggggac tcactggatc ggctagacga ctccccgtcc caagggcaaa gatccattct
     1861 ctggaattcg ccgccaccat gcaccaccac catcaccacg ttaacatgta caatgcaacc
     1921 aactccagga gcagaatgtt tagatatgaa gtagtaggac tgaggcagac tgccgaaacc
     1981 gaaaagacca actatgccat acgaaatagc ggaagccagt tttttaacgt accctatgat
     2041 aggatgaatc agttcatgca gcagataaca cgctggggcg gaaagatcgt atccattcag
     2101 cccttgaacg gcacagtggc tcccctggct gccaccacgg aacccgctgc aaataatggc
     2161 gctgcgcccg tcaaagagaa aaaggttgat ataccagtta acatctatcg cccgaacaac
     2221 ccctgtattg gaaaagtaat atccaacgag gaactggtga gggagggcgg tgagggaacc
     2281 gtgaaacaca ttatttttga tatctctgga accgagctca gatatttgga gggccagtct
     2341 atcgggatca ttccggctgg gaccgacgca aatggtaaac cacataaact ccggctgtat
     2401 tctatcgcct caacgaggca cggggacttt caggatgaca agaccgtctc actgtgcgtt
     2461 agacgcctcg aatataaaga taaagagact ggcgagacca tttatggcgt gtgctcctcc
     2521 tacctgaatc agctgcagcc tggagacgaa gtgaagatta ctggtcccgt tgggaaggaa
     2581 atgctgttga gtgacgaccc tgaagccacc atcataatgc ttgctaccgg caccggaatt
     2641 gccccctttc gcgctttctt gtggcggatg ttcaaagaaa acaacccaga ttatcagttc
     2701 aaaggacttg cctggctgtt ttttggcgtc gcctacaccg ctaacatcct gtataaagat
     2761 gagctggagg ccatccaggc ccagtatcct gatcactttc ggcttacata cgcaatttca
     2821 cgagagcaga agactccgga cggtgggaaa atgtacatcc aggggcgaat cgccgaacat
     2881 gctgacgaga tttggcagtt gcttcagaaa aaaaacacac acgtctatat gtgcggcctg
     2941 aggggcatgg aacctgggat agatgaagca atgacagcag ccgccgccaa gaatggcgca
     3001 gattggcagg aatttcttaa aggaacattg aagaaagagg gacggtggca cgtggaaaca
     3061 tattaataag tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     3121 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     3181 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     3241 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3301 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3361 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     3421 taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     3481 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     3541 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     3601 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     3661 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     3721 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     3781 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     3841 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     3901 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     3961 cgccgacgtc gagtgcccga aggaccgcgc gacctggtgc atgacccgca agcccggtgc
     4021 ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc
     4081 gataaaataa aagattttat ttagtctcca gaaaaagggg gcggccgctc gagacctaga
     4141 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
     4201 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
     4261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     4321 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     4381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     4441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     4501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     4561 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     4621 gccttgacat tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag
     4681 ttctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4741 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4801 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4861 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     4921 gggctctatg gcttctgagg cggaaagaac cagctggggg cgcgcccctc gaggccgcca
     4981 tggtcatagc tgtttgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5041 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5101 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5161 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5221 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5281 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5341 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5401 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5461 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5521 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5581 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5641 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5701 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5761 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5821 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5881 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5941 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6001 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6061 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6121 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6181 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6241 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6301 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6361 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6421 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6481 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6541 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6601 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6661 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6721 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6781 cctggccttt tgccggaccg ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     6841 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     6901 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     6961 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7021 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7081 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     7141 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     7201 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     7261 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     7321 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     7381 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7441 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7501 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     7561 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     7621 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     7681 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     7741 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     7801 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     7861 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     7921 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     7981 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8041 ccgcacagca accggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     8101 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     8161 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     8221 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     8281 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     8341 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     8401 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     8461 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     8521 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     8581 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     8641 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     8701 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     8761 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     8821 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     8881 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ccacaaatgg
     8941 cagtattcat ccacaatttt aaaagaaagg gggggattgg ggggtacagt gcaggggaaa
     9001 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     9061 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg tcgataagct
     9121 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     9181 gtcttgaaag gagtgggaat t
//
