LOCUS       40924_34719       10559 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA728, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Constitutive expression of eIF5A3 increases high quality biomass 
            yield in an elite alfalfa cultivar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2015) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10559)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2015) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        362..742
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             762..1550
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1571..1823
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      1880..2823
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     CDS             2875..3354
                     /codon_start=1
                     /gene="eIF5A3"
                     /product="eukaryotic translation initiation factor 5A"
                     /label=eIF5A3
                     /note="eIF5A3; derived from Populus deltoides"
                     /protein_id="AKE42819.1"
                     /translation="MSDEEQHFESKADAGASKTYPQQAGTIRKSGYIVIKNRPCKVVEV
                     STSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSL
                     LTENGNTKDDLRLPTDESLLSQIKDGFGEGKDLVVTVMSSMGEEQICALKDVGPK"
     gene            2875..3354
                     /gene="eIF5A3"
                     /label=eIF5A3
                     /note="PdeIF5A3"
     regulatory      3377..3680
                     /label=pea E9
                     /note="pea E9"
                     /regulatory_class="terminator"
     misc_feature    3767..3791
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5243..5869
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6301..7371
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7440..7634
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8108..8248
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8434..9022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9490..10281)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctagatgta cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
       61 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      121 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      181 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      241 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      301 tgcggacgtt tttaatgtac tgaattaacg ccgaatttgt acaccgcgga tcgattgaga
      361 tttttcaaat cagtgcgcaa gacgtgacgt aagtatctga gctagttttt atttttctac
      421 taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat ttcgcgggta
      481 ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt gaatagatac
      541 gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac agcaagaacg
      601 gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga taaatagcct
      661 tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg aatttcataa
      721 ccaatctcga tacaccaaat cgaatcgata ccggtatggg gattgaacaa gatggattgc
      781 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      841 caatcggctg ctctgatgcc gccgtgtttc ggctgtcagc gcaggggcgc ccggttcttt
      901 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca ggacgaggca gcgcggctat
      961 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1021 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1081 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1141 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1201 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1261 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac
     1321 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1381 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1441 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1501 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaaccggtg
     1561 cgatcgcaat cttggactcc catgttggca aaggcaacca aacaaacaat gaatgatccg
     1621 ctcctgcata tggggcggtt tgagtatttc aactgccatt tgggctgaat tgaagacatg
     1681 ctcctgtcag aaattccgtg atcttactca atattcagta atctcggcca atatcctaaa
     1741 tgtgcgtggc tttatctgtc tttgtattgt ttcatcaatt catgtaacgt ttgcttttct
     1801 tatgaatttt caaataaatt atcagatcgc gatcgcccgc ggtgactgac tgacttaatt
     1861 aatgcatggc gccatcgatg aattcagaca aatttgggaa aaatccagat tatctgatct
     1921 tgaagatgga gtcgctgaaa aattctacac agaagataaa gctacaaaat ccctcttcac
     1981 attcacagaa aatgcagaac catatcttgt tcatgcagct ttccgtgcag gattagcaaa
     2041 ggtaatctac cctagtccaa atctccaaga actcaaatgg ttcccaagag atatcgttaa
     2101 ggcaatcaaa catttccgga agaaagtgtt aaacgccaaa gactcagcaa tctacatcaa
     2161 gattttcagc agtctaccag attggaaaca atcagaaagg ttcacgcctt accatttctt
     2221 gcaaattggt atttccaaag caaagaaaga gctcccaagc acgaaggcat gtgtcggaaa
     2281 agattttcca accgagcagc agatggcagc aatcagatca tcaagtctga aaaccatagt
     2341 cgaaaaatta caggagataa aaaatgactc aactgtaaaa atcaactatg actcaagcac
     2401 atgcatcatc tcgagcaagt gtacaaaaaa gattaccacg gaagaaattc acaaggtaaa
     2461 actttttgag aaaagattga tcaacatcaa tcaaatcaat tgtggagacc acacaaagaa
     2521 attgtggtgt aaaattctac agaaaaagta cgaagagcat ctttgccagt actgcagtgg
     2581 ggaccaccca cgtgacaaca aagaagtggc agacaagtca cctcaaagtg gaacccatcc
     2641 acgtgataaa agtatcatgg cagacagccg gtacaataat ggggaggaca gcttgcaaag
     2701 cagcccatgt ggaagcccac tcacaaacgc gtattacgaa cgcagtgacg acatccactc
     2761 aagaattcca tctatttaaa gacggattca ttcccatttg aagatcatca atactcaacc
     2821 aatatcgatg atatccaaag ctttggcggc cgcactctct tatcaatcgc ggccatgtct
     2881 gacgaggagc agcacttcga gtcaaaagct gatgcgggag cttcgaaaac ttaccctcaa
     2941 caagctggta ccattcgcaa gagcggttac attgtcatca agaatcgccc ttgcaaggtt
     3001 gtggaggttt ctacctctaa aactggcaag cacggccatg ccaaatgtca ctttgttgca
     3061 attgatatct tcaatggaaa aaagcttgaa gatattgttc cttcttccca caactgtgat
     3121 gttccccatg tcacccgtac tgactatcag ctgattgata tctcagagga tggatttgtg
     3181 agcttgctga ctgagaatgg caataccaag gatgacctga ggctcccaac tgatgagagt
     3241 ctcctctctc agatcaagga tggatttggc gagggtaaag atcttgttgt gactgtgatg
     3301 tcctccatgg gagaggagca gatctgcgcc ctcaaggacg ttggcccgaa gtaaattggg
     3361 atccccgggt accgcgatcg cttcgagtat tatggcattg ggaaaactgt ttttcttgta
     3421 ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat cgaactgtga
     3481 aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca ttctcaaatt
     3541 aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat aagagatatg
     3601 caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac cgaagttaat
     3661 atgaggagta aaacatcgcg atcgcggcgc catgcattaa ttaacaattg agcttgagct
     3721 tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac aggatatatt
     3781 ggcgggtaaa cctaagagaa aagagcgttt attagaataa cggatattta aaagggcgtg
     3841 aaaaggttta tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga
     3901 tcaacaattg tctagacatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     3961 gtctcgcgcg tttcggtgat gacggtgagc gctaaaacct ctgacacatg cagctcccgg
     4021 agacggtcac agcttgtctg taagcggatg ccgggagcag atagggccca acccctccgc
     4081 tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg
     4141 cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc
     4201 gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg
     4261 aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac
     4321 ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag
     4381 atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct
     4441 ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg
     4501 gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg
     4561 gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag
     4621 ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga
     4681 ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag
     4741 ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc
     4801 tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg
     4861 cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat
     4921 gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca
     4981 ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc
     5041 acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg
     5101 cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg
     5161 tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata
     5221 aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc
     5281 aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt
     5341 tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga
     5401 tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat
     5461 cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc
     5521 cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg
     5581 ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct
     5641 acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc
     5701 cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag
     5761 ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc
     5821 tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga
     5881 ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc
     5941 agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac
     6001 tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag
     6061 accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga
     6121 ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc
     6181 aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag
     6241 cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc
     6301 gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg
     6361 gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc
     6421 cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg
     6481 gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc
     6541 gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt
     6601 ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac
     6661 gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg
     6721 gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga
     6781 gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga
     6841 gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg
     6901 cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag
     6961 ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac
     7021 atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac
     7081 gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac
     7141 cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac
     7201 gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc
     7261 gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc
     7321 ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg
     7381 gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct
     7441 gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt
     7501 gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag
     7561 aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc
     7621 ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc
     7681 cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc
     7741 cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg
     7801 tcgccactcg accgccggcg ctgggccctt attgaagcat ttatcagggt tattgtctca
     7861 tgagcggata catatttgaa tgtatttaga aaaaccatgg taaacaaata ggggttccgc
     7921 gcacatttcc ccgaaaagtg ccaccttaaa ccattattat gacgtcctgc ctcgcgcgtt
     7981 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     8041 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     8101 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
     8161 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
     8221 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct
     8281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     8881 atttggtatc tccgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9061 gtggaacgaa aactcacgtt aagggatttt ggtcatggac gtcttcctcg ctcactgact
     9121 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaggcg
     9181 cgccatcgat ggcacgaacc cagtggacat aagcctcgtt cggttcgtaa gctgtaatgc
     9241 aagtagcgta actgccgtca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     9301 acggcgcagt ggcggttttc atggcttctt gttatgacat gtttttttgg ggtacagtct
     9361 atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg
     9421 agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa catcatcgat
     9481 accggtctaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
     9541 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
     9601 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
     9661 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
     9721 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
     9781 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
     9841 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
     9901 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
     9961 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    10021 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    10081 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    10141 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    10201 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    10261 tgatattctc attttagcca taccggtgcg atcgctgtct aacaattcgt tcaagccgac
    10321 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
    10381 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
    10441 aattgggaga tatatcatgg cgatcgcggc gcgcctgact gactgacata cggttatcca
    10501 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagg
//
