LOCUS       40924_34714       10181 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA726, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Constitutive expression of eIF5A3 increases high quality biomass 
            yield in an elite alfalfa cultivar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2015) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10181)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2015) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        362..742
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             762..1550
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      1571..1823
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      1874..2455
                     /label=histone H3
                     /note="histone H3"
                     /regulatory_class="promoter"
     CDS             2497..2976
                     /codon_start=1
                     /gene="eIF5A3"
                     /product="eukaryotic translation initiation factor 5A"
                     /label=eIF5A3
                     /note="eIF5A3; derived from Populus deltoides"
                     /protein_id="AKE42828.1"
                     /translation="MSDEEQHFESKADAGASKTYPQQAGTIRKSGYIVIKNRPCKVVEV
                     STSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSL
                     LTENGNTKDDLRLPTDESLLSQIKDGFGEGKDLVVTVMSSMGEEQICALKDVGPK"
     gene            2497..2976
                     /gene="eIF5A3"
                     /label=eIF5A3
                     /note="PdeIF5A3"
     regulatory      2999..3302
                     /label=pea E9
                     /note="pea E9"
                     /regulatory_class="terminator"
     misc_feature    3389..3413
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4865..5491
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5923..6993
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7062..7256
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7730..7870
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8056..8644)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9112..9903)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctagatgta cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
       61 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      121 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      181 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      241 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      301 tgcggacgtt tttaatgtac tgaattaacg ccgaatttgt acaccgcgga tcgattgaga
      361 tttttcaaat cagtgcgcaa gacgtgacgt aagtatctga gctagttttt atttttctac
      421 taatttggtc gtttatttcg gcgtgtagga catggcaacc gggcctgaat ttcgcgggta
      481 ttctgtttct attccaactt tttcttgatc cgcagccatt aacgactttt gaatagatac
      541 gctgacacgc caagcctcgc tagtcaaaag tgtaccaaac aacgctttac agcaagaacg
      601 gaatgcgcgt gacgctcgcg gtgacgccat ttcgcctttt cagaaatgga taaatagcct
      661 tgcttcctat tatatcttcc caaattacca atacattaca ctagcatctg aatttcataa
      721 ccaatctcga tacaccaaat cgaatcgata ccggtatggg gattgaacaa gatggattgc
      781 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
      841 caatcggctg ctctgatgcc gccgtgtttc ggctgtcagc gcaggggcgc ccggttcttt
      901 ttgtcaagac cgacctgtcc ggtgccctga atgaactcca ggacgaggca gcgcggctat
      961 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1021 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1081 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1141 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1201 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1261 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgacac
     1321 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1381 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     1441 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1501 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaaccggtg
     1561 cgatcgcaat cttggactcc catgttggca aaggcaacca aacaaacaat gaatgatccg
     1621 ctcctgcata tggggcggtt tgagtatttc aactgccatt tgggctgaat tgaagacatg
     1681 ctcctgtcag aaattccgtg atcttactca atattcagta atctcggcca atatcctaaa
     1741 tgtgcgtggc tttatctgtc tttgtattgt ttcatcaatt catgtaacgt ttgcttttct
     1801 tatgaatttt caaataaatt atcagatcgc gatcgcccgc ggtgactgac tgacttaatt
     1861 aatgcatggc gccatcgatg atatccaaag ctttacattc agggactatt ttgcaattta
     1921 cctgcattca gggactaaag tgacgacttc tttcctattc agagactaaa gtgaccaatc
     1981 tctcaaaatg aagatatttt gttttgtttt agcaggaatg cgaagagtta aaggcgagat
     2041 aagttgcaca cgatttacac tcacaaaaga aacacaaatt gtccacgctg gcaatccgca
     2101 actttacaaa ccaaccaatc agaaacaaac acacggatcg cacttaatat tttcacttaa
     2161 aaaactcatc attaccgttg aagcattcaa agtccacgat ctctctactc taattaacct
     2221 tccttaatca tcattaaccc ttgcatatat aaacacactt ctcttcaaca accctcatta
     2281 cacatttctt ctctttcgct aaatctaatc aatctttccc tctcttcgag ctttctctct
     2341 ccgattccca taggtaacca ccgttcaccg ccgtaacggt tttttcttct ttctgttttc
     2401 ttgatcttag ggtttcgttt tcttcaattc gaattttttg attgatttca tcgatttttt
     2461 ggttcaggcg gccgcactct cttatcaatc gcggccatgt ctgacgagga gcagcacttc
     2521 gagtcaaaag ctgatgcggg agcttcgaaa acttaccctc aacaagctgg taccattcgc
     2581 aagagcggtt acattgtcat caagaatcgc ccttgcaagg ttgtggaggt ttctacctct
     2641 aaaactggca agcacggcca tgccaaatgt cactttgttg caattgatat cttcaatgga
     2701 aaaaagcttg aagatattgt tccttcttcc cacaactgtg atgttcccca tgtcacccgt
     2761 actgactatc agctgattga tatctcagag gatggatttg tgagcttgct gactgagaat
     2821 ggcaatacca aggatgacct gaggctccca actgatgaga gtctcctctc tcagatcaag
     2881 gatggatttg gcgagggtaa agatcttgtt gtgactgtga tgtcctccat gggagaggag
     2941 cagatctgcg ccctcaagga cgttggcccg aagtaaattg ggatccccgg gtaccgcgat
     3001 cgcttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
     3061 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
     3121 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
     3181 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
     3241 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacatcg
     3301 cgatcgcggc gccatgcatt aattaacaat tgagcttgag cttggatcag attgtcgttt
     3361 cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag
     3421 aaaagagcgt ttattagaat aacggatatt taaaagggcg tgaaaaggtt tatccgttcg
     3481 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaacaat tgtctagaca
     3541 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     3601 atgacggtga gcgctaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3661 tgtaagcgga tgccgggagc agatagggcc caacccctcc gctgctatag tgcagtcggc
     3721 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     3781 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     3841 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     3901 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     3961 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     4021 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     4081 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     4141 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     4201 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4261 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4321 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4381 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4441 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     4501 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     4561 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     4621 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     4681 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     4741 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     4801 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     4861 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     4921 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     4981 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     5041 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     5101 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     5161 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     5221 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5281 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5341 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5401 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     5461 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     5521 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     5581 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     5641 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     5701 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     5761 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     5821 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     5881 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     5941 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     6001 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     6061 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     6121 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     6181 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6241 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6301 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6361 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6421 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     6481 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     6541 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     6601 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     6661 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     6721 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     6781 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     6841 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     6901 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     6961 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     7021 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     7081 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     7141 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     7201 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7261 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7321 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7381 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7441 cgctgggccc ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     7501 aatgtattta gaaaaaccat ggtaaacaaa taggggttcc gcgcacattt ccccgaaaag
     7561 tgccacctta aaccattatt atgacgtcct gcctcgcgcg tttcggtgat gacggtgaaa
     7621 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7681 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
     7741 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
     7801 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7861 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7921 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7981 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8041 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8101 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8161 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8221 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8281 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8341 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8401 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8461 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctccgctct
     8521 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8581 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8641 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8701 ttaagggatt ttggtcatgg acgtcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8761 ggctgcggcg agcggtatca gctcactcaa aggcggtagg cgcgccatcg atggcacgaa
     8821 cccagtggac ataagcctcg ttcggttcgt aagctgtaat gcaagtagcg taactgccgt
     8881 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
     8941 tcatggcttc ttgttatgac atgttttttt ggggtacagt ctatgcctcg ggcatccaag
     9001 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     9061 cagcagggca gtcgccctaa aacaaagtta aacatcatcg ataccggtct aaaacaattc
     9121 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
     9181 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
     9241 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
     9301 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
     9361 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
     9421 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
     9481 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
     9541 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
     9601 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
     9661 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
     9721 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
     9781 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
     9841 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
     9901 cataccggtg cgatcgctgt ctaacaattc gttcaagccg acgccgcttc gccggcgtta
     9961 actcaagcga ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt
    10021 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat
    10081 ggcgatcgcg gcgcgcctga ctgactgaca tacggttatc cacagaatca ggggataacg
    10141 caggaaagaa catgtgagca aaaggccagc aaaaggccag g
//
