LOCUS       40924_34699       10675 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA713, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Constitutive expression of eIF5A3 increases high quality biomass 
            yield in an elite alfalfa cultivar
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10675)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Le H, Tricoli D, Sandman J, 
            Johnson D, Reich J, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2015) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10675)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10675)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-JAN-2015) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..406
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             420..1142
                     /codon_start=1
                     /gene="ipt"
                     /product="isopentenyl phosphotransferase"
                     /label=ipt
                     /note="involved in condensation of AMP and 
                     isopentenylpyrophosphate to form isopentenyl-AMP"
                     /protein_id="AKE42821.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     gene            420..1142
                     /gene="ipt"
                     /label=ipt
     terminator      1505..1757
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    1782..1949
                     /label=T-DNA left border
                     /note="T-DNA left border"
     regulatory      1981..2924
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     CDS             2976..3455
                     /codon_start=1
                     /gene="eIF5A3"
                     /product="eukaryotic translation initiation factor 5A"
                     /label=eIF5A3
                     /note="eIF5A3; derived from Populus deltoides"
                     /protein_id="AKE42822.1"
                     /translation="MSDEEQHFESKADAGASKTYPQQAGTIRKSGYIVIKNRPCKVVEV
                     STSKTGKHGHAKCHFVAIDIFNGKKLEDIVPSSHNCDVPHVTRTDYQLIDISEDGFVSL
                     LTENGNTKDDLRLPTDESLLSQIKDGFGEGKDLVVTVMSSMGEEQICALKDVGPK"
     gene            2976..3455
                     /gene="eIF5A3"
                     /label=eIF5A3
                     /note="PdeIF5A3"
     regulatory      3478..3781
                     /label=pea E9
                     /note="pea E9"
                     /regulatory_class="terminator"
     misc_feature    3812..4020
                     /label=T-DNA left border
                     /note="T-DNA left border"
     CDS             5359..5985
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6417..7487
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7556..7750
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8224..8364
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8550..9138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9606..10397)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctaggtcgg cggatcgatt gagatttttc aaatcagtgc gcaagacgtg acgtaagtat
       61 ctgagctagt ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc
      121 aaccgggcct gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc
      181 cattaacgac ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc
      241 aaacaacgct ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc
      301 ttttcagaaa tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat
      361 tacactagca tctgaatttc ataaccaatc tcgatacacc aaatcgaatc gataccggta
      421 tggacctgca tctaattttc ggtccaactt gcacaggaaa gacgacgacc gcgatagctc
      481 ttgcccagca gacagggctt ccagtccttt cgcttgatcg ggtccaatgc tgtcctcaac
      541 tatcaaccgg aagcggacga ccaacagtgg aagaactgaa aggaacgacg cgtctctacc
      601 ttgatgatcg gcctctggtg gagggtatca tcgcagccaa gcaagctcat cataggctga
      661 tcgaggaggt gtataatcat gaggccaacg gcgggcttat tcttgaggga ggatccacct
      721 cgttgctcaa ctgcatggcg cgaaacagct attggagtgc agattttcgt tggcatatta
      781 ttcgccacaa gttacccgac caagagacct tcatgaaagc ggccaaggcc agagttaagc
      841 agatgttgca ccccgctgca ggccattcta ttattcaaga gttggtttat ctttggaatg
      901 aacctcggct gaggcccatt ctgaaagaga tcgatggata tcgatatgcc atgttgtttg
      961 ctagccagaa ccagatcacg gcagatatgc tattgcagct tgacgcaaat atggaaggta
     1021 agttgattaa tgggatcgct caggagtatt tcatccatgc gcgccaacag gaacagaaat
     1081 tcccccaagt taacgcagcc gctttcgacg gattcgaagg tcatccgttc ggaatgtatt
     1141 aggttacgcc agccctgcgt cgcacctgtc ttcatctgga taagatgttc gtaattgttt
     1201 ttggctttgt cctgttgtgg cagggcggca aatacttccg acaatccatc gtgtcttcaa
     1261 actttatgct ggtgaacaag tcttagtttc cacgaaagta ttatgttaaa ttttaaaatt
     1321 tcgatgtata atgtggctat aattgtaaaa ataaactatc gtaagtgtgc gtgttatgta
     1381 taatttgtct aaatgtttaa tatatatcat agaacgcaat aaatattaaa tatagcgctt
     1441 ttatgaaata taaatacatc attacaagtt gtttatattt cgggtggacc ggtgcgatcg
     1501 caatcttgga ctcccatgtt ggcaaaggca accaaacaaa caatgaatga tccgctcctg
     1561 catatggggc ggtttgagta tttcaactgc catttgggct gaattgaaga catgctcctg
     1621 tcagaaattc cgtgatctta ctcaatattc agtaatctcg gccaatatcc taaatgtgcg
     1681 tggctttatc tgtctttgta ttgtttcatc aattcatgta acgtttgctt ttcttatgaa
     1741 ttttcaaata aattatcaga tcgcgatcgc cccgatgtac atggctggaa gcttggcggc
     1801 aggatgtata cagaggtata caattttata ttacatttat atttgtgtta attcattgaa
     1861 ttttcacttt tattttttac tttgataatc aactgtgtaa agaattattt gaaaaatata
     1921 tataatttat agaatttttt tttgttatgt gtacatctag taatgcatgg cgccatcgat
     1981 gaattcagac aaatttggga aaaatccaga ttatctgatc ttgaagatgg agtcgctgaa
     2041 aaattctaca cagaagataa agctacaaaa tccctcttca cattcacaga aaatgcagaa
     2101 ccatatcttg ttcatgcagc tttccgtgca ggattagcaa aggtaatcta ccctagtcca
     2161 aatctccaag aactcaaatg gttcccaaga gatatcgtta aggcaatcaa acatttccgg
     2221 aagaaagtgt taaacgccaa agactcagca atctacatca agattttcag cagtctacca
     2281 gattggaaac aatcagaaag gttcacgcct taccatttct tgcaaattgg tatttccaaa
     2341 gcaaagaaag agctcccaag cacgaaggca tgtgtcggaa aagattttcc aaccgagcag
     2401 cagatggcag caatcagatc atcaagtctg aaaaccatag tcgaaaaatt acaggagata
     2461 aaaaatgact caactgtaaa aatcaactat gactcaagca catgcatcat ctcgagcaag
     2521 tgtacaaaaa agattaccac ggaagaaatt cacaaggtaa aactttttga gaaaagattg
     2581 atcaacatca atcaaatcaa ttgtggagac cacacaaaga aattgtggtg taaaattcta
     2641 cagaaaaagt acgaagagca tctttgccag tactgcagtg gggaccaccc acgtgacaac
     2701 aaagaagtgg cagacaagtc acctcaaagt ggaacccatc cacgtgataa aagtatcatg
     2761 gcagacagcc ggtacaataa tggggaggac agcttgcaaa gcagcccatg tggaagccca
     2821 ctcacaaacg cgtattacga acgcagtgac gacatccact caagaattcc atctatttaa
     2881 agacggattc attcccattt gaagatcatc aatactcaac caatatcgat gatatccaaa
     2941 gctttggcgg ccgcactctc ttatcaatcg cggccatgtc tgacgaggag cagcacttcg
     3001 agtcaaaagc tgatgcggga gcttcgaaaa cttaccctca acaagctggt accattcgca
     3061 agagcggtta cattgtcatc aagaatcgcc cttgcaaggt tgtggaggtt tctacctcta
     3121 aaactggcaa gcacggccat gccaaatgtc actttgttgc aattgatatc ttcaatggaa
     3181 aaaagcttga agatattgtt ccttcttccc acaactgtga tgttccccat gtcacccgta
     3241 ctgactatca gctgattgat atctcagagg atggatttgt gagcttgctg actgagaatg
     3301 gcaataccaa ggatgacctg aggctcccaa ctgatgagag tctcctctct cagatcaagg
     3361 atggatttgg cgagggtaaa gatcttgttg tgactgtgat gtcctccatg ggagaggagc
     3421 agatctgcgc cctcaaggac gttggcccga agtaaattgg gatccccggg taccgcgatc
     3481 gcttcgagta ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa
     3541 tttactgtgt tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa
     3601 gagttaatga atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct
     3661 cttatttgtt gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt
     3721 aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacatcgc
     3781 gatcgcggcg ccatgcatta ctagacaatt gttatgcggg ctaacgggct gcccgcggcc
     3841 ctttcgggct agccctaacg ggtaccgggc cccggcagga tgtatacaga ggtatacact
     3901 agttgtgaat aagtcgctgt gtatgtttgt ttgagatctc taagagaaaa gagcgtttat
     3961 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     4021 caattgccta gacatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4081 cgcgcgtttc ggtgatgacg gtgagcgcta aaacctctga cacatgcagc tcccggagac
     4141 ggtcacagct tgtctgtaag cggatgccgg gagcagatag ggcccaaccc ctccgctgct
     4201 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
     4261 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
     4321 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
     4381 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
     4441 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
     4501 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
     4561 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
     4621 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
     4681 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
     4741 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
     4801 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
     4861 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
     4921 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
     4981 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
     5041 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
     5101 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     5161 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     5221 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     5281 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     5341 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     5401 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     5461 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     5521 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     5581 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     5641 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     5701 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     5761 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     5821 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     5881 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     5941 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     6001 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     6061 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     6121 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     6181 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     6241 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     6301 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     6361 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     6421 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     6481 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     6541 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     6601 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     6661 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     6721 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     6781 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     6841 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     6901 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     6961 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     7021 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     7081 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     7141 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     7201 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     7261 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     7321 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
     7381 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
     7441 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
     7501 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
     7561 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
     7621 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
     7681 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
     7741 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
     7801 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
     7861 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
     7921 cactcgaccg ccggcgctgg gcccttattg aagcatttat cagggttatt gtctcatgag
     7981 cggatacata tttgaatgta tttagaaaaa ccatggtaaa caaatagggg ttccgcgcac
     8041 atttccccga aaagtgccac cttaaaccat tattatgacg tcctgcctcg cgcgtttcgg
     8101 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     8161 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     8221 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg
     8281 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     8341 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8401 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8461 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8521 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8581 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8641 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8701 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8761 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8821 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8881 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8941 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     9001 ggtatctccg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9061 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9121 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9181 aacgaaaact cacgttaagg gattttggtc atggacgtct tcctcgctca ctgactcgct
     9241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taggcgcgcc
     9301 atcgatggca cgaacccagt ggacataagc ctcgttcggt tcgtaagctg taatgcaagt
     9361 agcgtaactg ccgtcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     9421 cgcagtggcg gttttcatgg cttcttgtta tgacatgttt ttttggggta cagtctatgc
     9481 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     9541 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atcgataccg
     9601 gtctaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
     9661 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
     9721 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
     9781 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
     9841 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
     9901 ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
     9961 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
    10021 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
    10081 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
    10141 ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt tccttccagc
    10201 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
    10261 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
    10321 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
    10381 attctcattt tagccatacc ggtgcgatcg ctgtctaaca attcgttcaa gccgacgccg
    10441 cttcgccggc gttaactcaa gcgattagat gcactaagca cataattgct cacagccaaa
    10501 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
    10561 gggagatata tcatggcgat cgcggcgcgc ctgactgact gacatacggt tatccacaga
    10621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccagg
//
