LOCUS       40924_34689       10380 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA588, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Enabling Technologies for Plant Transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 10380)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10380)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..406
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             420..1142
                     /codon_start=1
                     /gene="ipt"
                     /product="isopentenyl phosphotransferase"
                     /label=ipt
                     /note="condensation of AMP and isopentenylpyrophosphate to
                     form isopentenyl-AMP, a cytokinin"
                     /protein_id="AFI23680.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     gene            420..1142
                     /gene="ipt"
                     /label=ipt
     terminator      1505..1757
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    2026..2050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      2168..3111
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     regulatory      3186..3489
                     /label=Pea E9 3' UTR
                     /note="Pea E9 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    3576..3600
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5064..5690
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             6122..7192
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      7261..7455
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7929..8069
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8255..8843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9311..10102)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctaggtcgg cggatcgatt gagatttttc aaatcagtgc gcaagacgtg acgtaagtat
       61 ctgagctagt ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc
      121 aaccgggcct gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc
      181 cattaacgac ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc
      241 aaacaacgct ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc
      301 ttttcagaaa tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat
      361 tacactagca tctgaatttc ataaccaatc tcgatacacc aaatcgaatc gataccggta
      421 tggacctgca tctaattttc ggtccaactt gcacaggaaa gacgacgacc gcgatagctc
      481 ttgcccagca gacagggctt ccagtccttt cgcttgatcg ggtccaatgc tgtcctcaac
      541 tatcaaccgg aagcggacga ccaacagtgg aagaactgaa aggaacgacg cgtctctacc
      601 ttgatgatcg gcctctggtg gagggtatca tcgcagccaa gcaagctcat cataggctga
      661 tcgaggaggt gtataatcat gaggccaacg gcgggcttat tcttgaggga ggatccacct
      721 cgttgctcaa ctgcatggcg cgaaacagct attggagtgc agattttcgt tggcatatta
      781 ttcgccacaa gttacccgac caagagacct tcatgaaagc ggccaaggcc agagttaagc
      841 agatgttgca ccccgctgca ggccattcta ttattcaaga gttggtttat ctttggaatg
      901 aacctcggct gaggcccatt ctgaaagaga tcgatggata tcgatatgcc atgttgtttg
      961 ctagccagaa ccagatcacg gcagatatgc tattgcagct tgacgcaaat atggaaggta
     1021 agttgattaa tgggatcgct caggagtatt tcatccatgc gcgccaacag gaacagaaat
     1081 tcccccaagt taacgcagcc gctttcgacg gattcgaagg tcatccgttc ggaatgtatt
     1141 aggttacgcc agccctgcgt cgcacctgtc ttcatctgga taagatgttc gtaattgttt
     1201 ttggctttgt cctgttgtgg cagggcggca aatacttccg acaatccatc gtgtcttcaa
     1261 actttatgct ggtgaacaag tcttagtttc cacgaaagta ttatgttaaa ttttaaaatt
     1321 tcgatgtata atgtggctat aattgtaaaa ataaactatc gtaagtgtgc gtgttatgta
     1381 taatttgtct aaatgtttaa tatatatcat agaacgcaat aaatattaaa tatagcgctt
     1441 ttatgaaata taaatacatc attacaagtt gtttatattt cgggtggacc ggtgcgatcg
     1501 caatcttgga ctcccatgtt ggcaaaggca accaaacaaa caatgaatga tccgctcctg
     1561 catatggggc ggtttgagta tttcaactgc catttgggct gaattgaaga catgctcctg
     1621 tcagaaattc cgtgatctta ctcaatattc agtaatctcg gccaatatcc taaatgtgcg
     1681 tggctttatc tgtctttgta ttgtttcatc aattcatgta acgtttgctt ttcttatgaa
     1741 ttttcaaata aattatcaga tcgcgatcgc cccgatgtac atctagatgt acacaggcag
     1801 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     1861 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     1921 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     1981 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     2041 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
     2101 ctgaattaac gccgaatttg tacaccgcgg tgactgactg acttaattaa tgcatggcgc
     2161 catcgatgaa ttcagacaaa tttgggaaaa atccagatta tctgatcttg aagatggagt
     2221 cgctgaaaaa ttctacacag aagataaagc tacaaaatcc ctcttcacat tcacagaaaa
     2281 tgcagaacca tatcttgttc atgcagcttt ccgtgcagga ttagcaaagg taatctaccc
     2341 tagtccaaat ctccaagaac tcaaatggtt cccaagagat atcgttaagg caatcaaaca
     2401 tttccggaag aaagtgttaa acgccaaaga ctcagcaatc tacatcaaga ttttcagcag
     2461 tctaccagat tggaaacaat cagaaaggtt cacgccttac catttcttgc aaattggtat
     2521 ttccaaagca aagaaagagc tcccaagcac gaaggcatgt gtcggaaaag attttccaac
     2581 cgagcagcag atggcagcaa tcagatcatc aagtctgaaa accatagtcg aaaaattaca
     2641 ggagataaaa aatgactcaa ctgtaaaaat caactatgac tcaagcacat gcatcatctc
     2701 gagcaagtgt acaaaaaaga ttaccacgga agaaattcac aaggtaaaac tttttgagaa
     2761 aagattgatc aacatcaatc aaatcaattg tggagaccac acaaagaaat tgtggtgtaa
     2821 aattctacag aaaaagtacg aagagcatct ttgccagtac tgcagtgggg accacccacg
     2881 tgacaacaaa gaagtggcag acaagtcacc tcaaagtgga acccatccac gtgataaaag
     2941 tatcatggca gacagccggt acaataatgg ggaggacagc ttgcaaagca gcccatgtgg
     3001 aagcccactc acaaacgcgt attacgaacg cagtgacgac atccactcaa gaattccatc
     3061 tatttaaaga cggattcatt cccatttgaa gatcatcaat actcaaccaa tatcgatgat
     3121 atccaaagct ttggcggccg cctgcaggtc gacgagctcg agaattcgga tccccgggta
     3181 ccgcgatcgc ttcgagtatt atggcattgg gaaaactgtt tttcttgtac catttgttgt
     3241 gcttgtaatt tactgtgttt tttattcggt tttcgctatc gaactgtgaa atggaaatgg
     3301 atggagaaga gttaatgaat gatatggtcc ttttgttcat tctcaaatta atattatttg
     3361 ttttttctct tatttgttgt gtgttgaatt tgaaattata agagatatgc aaacattttg
     3421 ttttgagtaa aaatgtgtca aatcgtggcc tctaatgacc gaagttaata tgaggagtaa
     3481 aacatcgcga tcgcggcgcc atgcattaat taacaattga gcttgagctt ggatcagatt
     3541 gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
     3601 ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga aaaggtttat
     3661 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caacaattgt
     3721 ctagacaatt gcctagacat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     3781 cgtctcgcgc gtttcggtga tgacggtgag cgctaaaacc tctgacacat gcagctcccg
     3841 gagacggtca cagcttgtct gtaagcggat gccgggagca gatagggccc aacccctccg
     3901 ctgctatagt gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc
     3961 gcacaagtcc taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt
     4021 cgcgtgtttt agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat
     4081 gaacaagagc gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga
     4141 cttgaccaac caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa
     4201 gatcaccggc accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc
     4261 tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact
     4321 ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg
     4381 ggccgacacc accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga
     4441 gttcgagcgt tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg
     4501 aggcgtgaag tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga
     4561 gctgatcgac caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg
     4621 ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg
     4681 gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa
     4741 tgaacgccaa gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc
     4801 attaccgaag agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg
     4861 cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg
     4921 gcctggccgg ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt
     4981 gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat
     5041 aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt
     5101 caggcaagac gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg
     5161 ttctgttagt cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag
     5221 atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca
     5281 tcggccggcg cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt
     5341 ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat
     5401 gggccaccgc cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc
     5461 tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg
     5521 ccgaggcgct ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga
     5581 gctacccagg cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg
     5641 ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg
     5701 aggtaaagag aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg
     5761 cagcagcaag gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa
     5821 ctttcagttg ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa
     5881 gaccattacc gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg
     5941 aataaatgag tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac
     6001 caggcaccga cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa
     6061 gcggctgggt tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg
     6121 cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
     6181 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
     6241 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
     6301 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
     6361 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
     6421 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
     6481 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
     6541 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
     6601 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
     6661 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
     6721 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
     6781 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
     6841 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
     6901 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
     6961 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
     7021 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
     7081 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
     7141 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
     7201 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
     7261 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
     7321 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
     7381 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
     7441 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
     7501 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
     7561 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
     7621 gtcgccactc gaccgccggc gctgggccct tattgaagca tttatcaggg ttattgtctc
     7681 atgagcggat acatatttga atgtatttag aaaaaccatg gtaaacaaat aggggttccg
     7741 cgcacatttc cccgaaaagt gccaccttaa accattatta tgacgtcctg cctcgcgcgt
     7801 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     7861 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     7921 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     7981 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     8041 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     8101 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8161 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8221 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8281 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8341 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8401 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8461 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8521 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8581 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8641 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     8701 tatttggtat ctccgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8761 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8821 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8881 agtggaacga aaactcacgt taagggattt tggtcatgga cgtcttcctc gctcactgac
     8941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaggc
     9001 gcgccatcga tggcacgaac ccagtggaca taagcctcgt tcggttcgta agctgtaatg
     9061 caagtagcgt aactgccgtc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     9121 aacggcgcag tggcggtttt catggcttct tgttatgaca tgtttttttg gggtacagtc
     9181 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     9241 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatcga
     9301 taccggtcta aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct
     9361 tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga
     9421 tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat
     9481 caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt
     9541 gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg
     9601 gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat
     9661 tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg
     9721 atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag
     9781 ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca
     9841 gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt
     9901 ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc
     9961 tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag
    10021 gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg
    10081 gtgatattct cattttagcc ataccggtgc gatcgctgtc taacaattcg ttcaagccga
    10141 cgccgcttcg ccggcgttaa ctcaagcgat tagatgcact aagcacataa ttgctcacag
    10201 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac
    10261 aaattgggag atatatcatg gcgatcgcgg cgcgcctgac tgactgacat acggttatcc
    10321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
//
