LOCUS       40924_34684       12429 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPIPRA587, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Enabling Technologies for Plant Transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   Figueroa-Balderas RE, Chi-Ham CL, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2011) Plant Sciences, UC Davis, One shields 
            Avenue, Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 12429)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12429)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-DEC-2011) Plant Sciences, UC Davis, One shields Avenue, Davis, 
            CA 95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..406
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             420..1142
                     /codon_start=1
                     /gene="ipt"
                     /product="isopentenyl phosphotransferase"
                     /label=ipt
                     /note="condensation of AMP and isopentenylpyrophosphate to
                     form isopentenyl-AMP, a cytokinin"
                     /protein_id="AFI23677.1"
                     /translation="MDLHLIFGPTCTGKTTTAIALAQQTGLPVLSLDRVQCCPQLSTGS
                     GRPTVEELKGTTRLYLDDRPLVEGIIAAKQAHHRLIEEVYNHEANGGLILEGGSTSLLN
                     CMARNSYWSADFRWHIIRHKLPDQETFMKAAKARVKQMLHPAAGHSIIQELVYLWNEPR
                     LRPILKEIDGYRYAMLFASQNQITADMLLQLDANMEGKLINGIAQEYFIHARQQEQKFP
                     QVNAAAFDGFEGHPFGMY"
     gene            420..1142
                     /gene="ipt"
                     /label=ipt
     terminator      1505..1757
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    2026..2050
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      2137..3080
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     CDS             3099..3887
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      3897..4173
                     /label=Mas term 3' UTR
                     /note="Mas term 3' UTR"
                     /regulatory_class="terminator"
     terminator      3908..4160
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     regulatory      4127..5160
                     /label=FMV34S
                     /note="FMV34S"
                     /regulatory_class="promoter"
     misc_feature    5172..5226
                     /label=MCS (polylinker)
                     /note="MCS (polylinker)"
     regulatory      5235..5538
                     /label=Pea E9 3' UTR
                     /note="Pea E9 3' UTR"
                     /regulatory_class="terminator"
     misc_feature    5625..5649
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7113..7739
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             8171..9241
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      9310..9504
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9978..10118
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10304..10892)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11360..12151)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctaggtcgg cggatcgatt gagatttttc aaatcagtgc gcaagacgtg acgtaagtat
       61 ctgagctagt ttttattttt ctactaattt ggtcgtttat ttcggcgtgt aggacatggc
      121 aaccgggcct gaatttcgcg ggtattctgt ttctattcca actttttctt gatccgcagc
      181 cattaacgac ttttgaatag atacgctgac acgccaagcc tcgctagtca aaagtgtacc
      241 aaacaacgct ttacagcaag aacggaatgc gcgtgacgct cgcggtgacg ccatttcgcc
      301 ttttcagaaa tggataaata gccttgcttc ctattatatc ttcccaaatt accaatacat
      361 tacactagca tctgaatttc ataaccaatc tcgatacacc aaatcgaatc gataccggta
      421 tggacctgca tctaattttc ggtccaactt gcacaggaaa gacgacgacc gcgatagctc
      481 ttgcccagca gacagggctt ccagtccttt cgcttgatcg ggtccaatgc tgtcctcaac
      541 tatcaaccgg aagcggacga ccaacagtgg aagaactgaa aggaacgacg cgtctctacc
      601 ttgatgatcg gcctctggtg gagggtatca tcgcagccaa gcaagctcat cataggctga
      661 tcgaggaggt gtataatcat gaggccaacg gcgggcttat tcttgaggga ggatccacct
      721 cgttgctcaa ctgcatggcg cgaaacagct attggagtgc agattttcgt tggcatatta
      781 ttcgccacaa gttacccgac caagagacct tcatgaaagc ggccaaggcc agagttaagc
      841 agatgttgca ccccgctgca ggccattcta ttattcaaga gttggtttat ctttggaatg
      901 aacctcggct gaggcccatt ctgaaagaga tcgatggata tcgatatgcc atgttgtttg
      961 ctagccagaa ccagatcacg gcagatatgc tattgcagct tgacgcaaat atggaaggta
     1021 agttgattaa tgggatcgct caggagtatt tcatccatgc gcgccaacag gaacagaaat
     1081 tcccccaagt taacgcagcc gctttcgacg gattcgaagg tcatccgttc ggaatgtatt
     1141 aggttacgcc agccctgcgt cgcacctgtc ttcatctgga taagatgttc gtaattgttt
     1201 ttggctttgt cctgttgtgg cagggcggca aatacttccg acaatccatc gtgtcttcaa
     1261 actttatgct ggtgaacaag tcttagtttc cacgaaagta ttatgttaaa ttttaaaatt
     1321 tcgatgtata atgtggctat aattgtaaaa ataaactatc gtaagtgtgc gtgttatgta
     1381 taatttgtct aaatgtttaa tatatatcat agaacgcaat aaatattaaa tatagcgctt
     1441 ttatgaaata taaatacatc attacaagtt gtttatattt cgggtggacc ggtgcgatcg
     1501 caatcttgga ctcccatgtt ggcaaaggca accaaacaaa caatgaatga tccgctcctg
     1561 catatggggc ggtttgagta tttcaactgc catttgggct gaattgaaga catgctcctg
     1621 tcagaaattc cgtgatctta ctcaatattc agtaatctcg gccaatatcc taaatgtgcg
     1681 tggctttatc tgtctttgta ttgtttcatc aattcatgta acgtttgctt ttcttatgaa
     1741 ttttcaaata aattatcaga tcgcgatcgc cccgatgtac atctagatgt acacaggcag
     1801 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa
     1861 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg
     1921 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg
     1981 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg
     2041 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta
     2101 ctgaattaac gccgaatttg tacaccgcgg atcgatgaat tcagacaaat ttgggaaaaa
     2161 tccagattat ctgatcttga agatggagtc gctgaaaaat tctacacaga agataaagct
     2221 acaaaatccc tcttcacatt cacagaaaat gcagaaccat atcttgttca tgcagctttc
     2281 cgtgcaggat tagcaaaggt aatctaccct agtccaaatc tccaagaact caaatggttc
     2341 ccaagagata tcgttaaggc aatcaaacat ttccggaaga aagtgttaaa cgccaaagac
     2401 tcagcaatct acatcaagat tttcagcagt ctaccagatt ggaaacaatc agaaaggttc
     2461 acgccttacc atttcttgca aattggtatt tccaaagcaa agaaagagct cccaagcacg
     2521 aaggcatgtg tcggaaaaga ttttccaacc gagcagcaga tggcagcaat cagatcatca
     2581 agtctgaaaa ccatagtcga aaaattacag gagataaaaa atgactcaac tgtaaaaatc
     2641 aactatgact caagcacatg catcatctcg agcaagtgta caaaaaagat taccacggaa
     2701 gaaattcaca aggtaaaact ttttgagaaa agattgatca acatcaatca aatcaattgt
     2761 ggagaccaca caaagaaatt gtggtgtaaa attctacaga aaaagtacga agagcatctt
     2821 tgccagtact gcagtgggga ccacccacgt gacaacaaag aagtggcaga caagtcacct
     2881 caaagtggaa cccatccacg tgataaaagt atcatggcag acagccggta caataatggg
     2941 gaggacagct tgcaaagcag cccatgtgga agcccactca caaacgcgta ttacgaacgc
     3001 agtgacgaca tccactcaag aattccatct atttaaagac ggattcattc ccatttgaag
     3061 atcatcaata ctcaaccaat atcgataccg gtatggggat tgaacaagat ggattgcacg
     3121 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3181 tcggctgctc tgatgccgcc gtgtttcggc tgtcagcgca ggggcgcccg gttctttttg
     3241 tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg cggctatcgt
     3301 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3361 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3421 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3481 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3541 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     3601 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacacatg
     3661 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3721 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3781 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     3841 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga accggtgcga
     3901 tcgcaatctt ggactcccat gttggcaaag gcaaccaaac aaacaatgaa tgatccgctc
     3961 ctgcatatgg ggcggtttga gtatttcaac tgccatttgg gctgaattga agacatgctc
     4021 ctgtcagaaa ttccgtgatc ttactcaata ttcagtaatc tcggccaata tcctaaatgt
     4081 gcgtggcttt atctgtcttt gtattgtttc atcaattcat gtaacgtttg cttttcttat
     4141 gaattttcaa ataaattatc agatcgcgat cgcccgcggt gactgactga cttaattaat
     4201 gcatggcgcc atcgatgaat tcagacaaat ttgggaaaaa tccagattat ctgatcttga
     4261 agatggagtc gctgaaaaat tctacacaga agataaagct acaaaatccc tcttcacatt
     4321 cacagaaaat gcagaaccat atcttgttca tgcagctttc cgtgcaggat tagcaaaggt
     4381 aatctaccct agtccaaatc tccaagaact caaatggttc ccaagagata tcgttaaggc
     4441 aatcaaacat ttccggaaga aagtgttaaa cgccaaagac tcagcaatct acatcaagat
     4501 tttcagcagt ctaccagatt ggaaacaatc agaaaggttc acgccttacc atttcttgca
     4561 aattggtatt tccaaagcaa agaaagagct cccaagcacg aaggcatgtg tcggaaaaga
     4621 ttttccaacc gagcagcaga tggcagcaat cagatcatca agtctgaaaa ccatagtcga
     4681 aaaattacag gagataaaaa atgactcaac tgtaaaaatc aactatgact caagcacatg
     4741 catcatctcg agcaagtgta caaaaaagat taccacggaa gaaattcaca aggtaaaact
     4801 ttttgagaaa agattgatca acatcaatca aatcaattgt ggagaccaca caaagaaatt
     4861 gtggtgtaaa attctacaga aaaagtacga agagcatctt tgccagtact gcagtgggga
     4921 ccacccacgt gacaacaaag aagtggcaga caagtcacct caaagtggaa cccatccacg
     4981 tgataaaagt atcatggcag acagccggta caataatggg gaggacagct tgcaaagcag
     5041 cccatgtgga agcccactca caaacgcgta ttacgaacgc agtgacgaca tccactcaag
     5101 aattccatct atttaaagac ggattcattc ccatttgaag atcatcaata ctcaaccaat
     5161 atcgatgata tccaaagctt tggcggccgc ctgcaggtcg acgagctcga gaattcggat
     5221 ccccgggtac cgcgatcgct tcgagtatta tggcattggg aaaactgttt ttcttgtacc
     5281 atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg aactgtgaaa
     5341 tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt ctcaaattaa
     5401 tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa gagatatgca
     5461 aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg aagttaatat
     5521 gaggagtaaa acatcgcgat cgcggcgcca tgcattaatt aacaattgag cttgagcttg
     5581 gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg
     5641 cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa agggcgtgaa
     5701 aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc
     5761 aacaattgtc tagacaattg cctagacatg acattaacct ataaaaatag gcgtatcacg
     5821 aggccctttc gtctcgcgcg tttcggtgat gacggtgagc gctaaaacct ctgacacatg
     5881 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag atagggccca
     5941 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
     6001 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
     6061 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
     6121 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
     6181 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
     6241 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
     6301 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
     6361 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
     6421 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
     6481 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
     6541 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
     6601 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
     6661 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
     6721 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
     6781 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
     6841 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
     6901 ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
     6961 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
     7021 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
     7081 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
     7141 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
     7201 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
     7261 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
     7321 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
     7381 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
     7441 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
     7501 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
     7561 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
     7621 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
     7681 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
     7741 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
     7801 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
     7861 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
     7921 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
     7981 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
     8041 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
     8101 caggcgtaag cggctgggtt gtctgccggc cctgcaatgg cactggaacc cccaagcccg
     8161 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
     8221 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
     8281 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
     8341 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
     8401 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
     8461 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
     8521 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
     8581 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
     8641 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
     8701 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
     8761 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
     8821 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
     8881 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
     8941 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
     9001 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
     9061 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
     9121 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
     9181 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
     9241 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
     9301 tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
     9361 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
     9421 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
     9481 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag agctgcaaaa
     9541 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
     9601 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
     9661 agccgcgccg tcgccactcg accgccggcg ctgggccctt attgaagcat ttatcagggt
     9721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaaccatgg taaacaaata
     9781 ggggttccgc gcacatttcc ccgaaaagtg ccaccttaaa ccattattat gacgtcctgc
     9841 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     9901 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     9961 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
    10021 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    10081 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    10141 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10201 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10261 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10321 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10381 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10441 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    10501 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    10561 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    10621 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    10681 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    10741 gaaggacagt atttggtatc tccgctctgc tgaagccagt taccttcgga aaaagagttg
    10801 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    10861 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10921 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatggac gtcttcctcg
    10981 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    11041 gcggtaggcg cgccatcgat ggcacgaacc cagtggacat aagcctcgtt cggttcgtaa
    11101 gctgtaatgc aagtagcgta actgccgtca cgcaactggt ccagaacctt gaccgaacgc
    11161 agcggtggta acggcgcagt ggcggttttc atggcttctt gttatgacat gtttttttgg
    11221 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
    11281 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
    11341 catcatcgat accggtctaa aacaattcat ccagtaaaat ataatatttt attttctccc
    11401 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
    11461 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
    11521 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    11581 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    11641 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    11701 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    11761 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    11821 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    11881 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    11941 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    12001 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    12061 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    12121 tcaattccgg tgatattctc attttagcca taccggtgcg atcgctgtct aacaattcgt
    12181 tcaagccgac gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat
    12241 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
    12301 gccctacaca aattgggaga tatatcatgg cgatcgcggc gcgcctgact gactgacata
    12361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    12421 aaggccagg
//
