LOCUS       40924_34674        9273 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pPIPRA560, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Chi-Ham CL, Boettiger S, Figueroa-Balderas R, Bird S, Geoola JN, 
            Zamora P, Alandete-Saez M, Bennett AB.
  TITLE     An intellectual property sharing initiative in agricultural 
            biotechnology: development of broadly accessible technologies for 
            plant transformation
  JOURNAL   Plant Biotechnol. J. 10 (5), 501-510 (2012)
  PUBMED    22221977
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Chi-Ham CL, Geoola JN, Figueroa-Balderas R, Bird S, Zamora P, 
            Boettiger S, Bennett AB.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue,
            Davis, CA 95616, USA
REFERENCE   3  (bases 1 to 9273)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9273)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol. J."; date: "2012"; volume: "10"; issue: "5"; pages: 
            "501-510"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-APR-2011) Plant Sciences, UCDavis, One Shields Avenue, Davis, CA
            95616, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    245..269
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      350..1300
                     /gene="FMV34S"
                     /regulatory_class="promoter"
     gene            350..1300
                     /gene="FMV34S"
                     /label=FMV34S
     CDS             1318..2106
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      2127..2379
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     misc_feature    2481..2505
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3957..4583
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5015..6085
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6154..6348
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6822..6962
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7148..7736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8204..8995)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tctagatgta cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
       61 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
      121 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
      181 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
      241 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
      301 tgcggacgtt tttaatgtac tgaattaacg ccgaatttgt acaccgcgga tcgatgaatt
      361 cagacaaatt tgggaaaaat ccagattatc tgatcttgaa gatggagtcg ctgaaaaatt
      421 ctacacagaa gataaagcta caaaatccct cttcacattc acagaaaatg cagaaccata
      481 tcttgttcat gcagctttcc gtgcaggatt agcaaaggta atctacccta gtccaaatct
      541 ccaagaactc aaatggttcc caagagatat cgttaaggca atcaaacatt tccggaagaa
      601 agtgttaaac gccaaagact cagcaatcta catcaagatt ttcagcagtc taccagattg
      661 gaaacaatca gaaaggttca cgccttacca tttcttgcaa attggtattt ccaaagcaaa
      721 gaaagagctc ccaagcacga aggcatgtgt cggaaaagat tttccaaccg agcagcagat
      781 ggcagcaatc agatcatcaa gtctgaaaac catagtcgaa aaattacagg agataaaaaa
      841 tgactcaact gtaaaaatca actatgactc aagcacatgc atcatctcga gcaagtgtac
      901 aaaaaagatt accacggaag aaattcacaa ggtaaaactt tttgagaaaa gattgatcaa
      961 catcaatcaa atcaattgtg gagaccacac aaagaaattg tggtgtaaaa ttctacagaa
     1021 aaagtacgaa gagcatcttt gccagtactg cagtggggac cacccacgtg acaacaaaga
     1081 agtggcagac aagtcacctc aaagtggaac ccatccacgt gataaaagta tcatggcaga
     1141 cagccggtac aataatgggg aggacagctt gcaaagcagc ccatgtggaa gcccactcac
     1201 aaacgcgtat tacgaacgca gtgacgacat ccactcaaga attccatcta tttaaagacg
     1261 gattcattcc catttgaaga tcatcaatac tcaaccaata tcgataccgg tatggggatt
     1321 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     1381 gactgggcac aacagacaat cggctgctct gatgccgccg tgtttcggct gtcagcgcag
     1441 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaggac
     1501 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     1561 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     1621 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     1681 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     1741 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     1801 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1861 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1921 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1981 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     2041 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     2101 ttcttctgaa ccggtgcgat cgcaatcttg gactcccatg ttggcaaagg caaccaaaca
     2161 aacaatgaat gatccgctcc tgcatatggg gcggtttgag tatttcaact gccatttggg
     2221 ctgaattgaa gacatgctcc tgtcagaaat tccgtgatct tactcaatat tcagtaatct
     2281 cggccaatat cctaaatgtg cgtggcttta tctgtctttg tattgtttca tcaattcatg
     2341 taacgtttgc ttttcttatg aattttcaaa taaattatca gatcgcgatc gcccgcggtg
     2401 actgactgac ttaattaaca attgagcttg agcttggatc agattgtcgt ttcccgcctt
     2461 cagtttaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     2521 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
     2581 tatgtgcatg ccaaccacag ggttcccctc gggatcaaca attgtctaga catgacatta
     2641 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     2701 gagcgctaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     2761 gatgccggga gcagataggg cccaacccct ccgctgctat agtgcagtcg gcttctgacg
     2821 ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc
     2881 tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa
     2941 tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct
     3001 gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca
     3061 cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc
     3121 ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct
     3181 agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc
     3241 cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg
     3301 catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg
     3361 cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac
     3421 cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt
     3481 gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg
     3541 cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt
     3601 gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa
     3661 ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg
     3721 atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa
     3781 atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa
     3841 gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat
     3901 gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga
     3961 aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc
     4021 tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg
     4081 gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg
     4141 accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg
     4201 acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc
     4261 tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg
     4321 ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg
     4381 cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc
     4441 ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca
     4501 caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg
     4561 ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac
     4621 aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag
     4681 cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac
     4741 caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata
     4801 catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg
     4861 ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca
     4921 tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca
     4981 atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc
     5041 ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc
     5101 cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc
     5161 tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa
     5221 gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac
     5281 ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg
     5341 agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc
     5401 ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac
     5461 cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc
     5521 acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga
     5581 cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa
     5641 ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa
     5701 gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat
     5761 gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt
     5821 tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa
     5881 ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt
     5941 caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga
     6001 tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat
     6061 cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct
     6121 agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc
     6181 aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa
     6241 ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa
     6301 ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca
     6361 gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc
     6421 cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc
     6481 aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgctgggc
     6541 ccttattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6601 tagaaaaacc atggtaaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6661 taaaccatta ttatgacgtc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     6721 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     6781 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     6841 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     6901 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     6961 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7021 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7081 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7141 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7201 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7261 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7321 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7381 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7441 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7501 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7561 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctccgct ctgctgaagc
     7621 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7681 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7741 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7801 ttttggtcat ggacgtcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7861 cgagcggtat cagctcactc aaaggcggta ggcgcgccat cgatggcacg aacccagtgg
     7921 acataagcct cgttcggttc gtaagctgta atgcaagtag cgtaactgcc gtcacgcaac
     7981 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     8041 tcttgttatg acatgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     8101 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     8161 cagtcgccct aaaacaaagt taaacatcat cgataccggt ctaaaacaat tcatccagta
     8221 aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct
     8281 cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat
     8341 accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt
     8401 tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct
     8461 tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc
     8521 agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc
     8581 ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga
     8641 tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg
     8701 agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa
     8761 agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc
     8821 gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt
     8881 cattttctcc caccagctta tataccttag caggagacat tccttccgta tcttttacgc
     8941 agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccataccgg
     9001 tgcgatcgct gtctaacaat tcgttcaagc cgacgccgct tcgccggcgt taactcaagc
     9061 gattagatgc actaagcaca taattgctca cagccaaact atcaggtcaa gtctgctttt
     9121 attattttta agcgtgcata ataagcccta cacaaattgg gagatatatc atggcgatcg
     9181 cggcgcgcct gactgactga catacggtta tccacagaat caggggataa cgcaggaaag
     9241 aacatgtgag caaaaggcca gcaaaaggcc agg
//
