LOCUS       40924_34639        4153 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pPILY, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Ferrando A, Farras R, Jasik J, Schell J, Koncz C.
  TITLE     Intron-tagged epitope: a tool for facile detection and purification 
            of proteins expressed in Agrobacterium-transformed plant cells
  JOURNAL   Plant J. 22 (6), 553-560 (2000)
  PUBMED    10886775
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Berendzen KW, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   3  (bases 1 to 4153)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2000"; volume: "22"; issue: "6"; pages: "553-560"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..805
                     /label=N-term HA epitope
                     /note="N-term HA epitope"
     intron          806..994
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    995..1009
                     /label=C-term HA epitope
                     /note="C-term HA epitope"
     misc_feature    1010..1037
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1038..1290
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1322..1340)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1370..1386)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1394..1410)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1418..1448)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1463..1484)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1772..2360)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2534..3391)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3392..3496)
                     /label=AmpR promoter
     rep_origin      3523..3978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4119..4135
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tcttatccat acgatgtaag tttctgcttc tacctttgat atatatataa
      841 taattatcat taattagtag taatataata ttttaaatat ttttttcaaa ataaaagaat
      901 gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatatttcaa tttataactt
      961 ttctaatata tgaccaaaat ttgttgatgt gcaggttcca gattatgctg agtcgacgga
     1021 tccactgcag tgaattctcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     1081 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     1141 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     1201 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     1261 cgcgcggtgt catctgtcga gggggggccc ggtaccgagc tcgcggccgc tcgacgagct
     1321 ccctatagtg agtcgtatta naggccgact tggccaaatt cgtaatcatg tcatagctgt
     1381 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     1441 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     1501 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     1561 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     1621 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1681 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1741 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1801 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1861 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1921 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1981 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2041 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2101 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2161 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2221 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2281 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2341 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2401 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2461 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2521 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     2581 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     2641 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2701 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2761 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2821 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2881 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2941 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     3001 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     3061 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     3121 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     3181 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     3241 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     3301 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     3361 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     3421 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     3481 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     3541 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     3601 tagggcccgg tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3661 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3721 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3841 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3961 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     4021 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     4081 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
     4141 gctgacttgg tca
//
