LOCUS       40924_34614        5475 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pPICZTS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             949..1215
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     CDS             1219..3150
                     /codon_start=1
                     /note="unnamed protein product; mature TS"
                     /protein_id="SJL87752.1"
                     /translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNVD
                     GVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVIVK
                     GNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVSLKK
                     FFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKTWKFG
                     KGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVWGPSPK
                     SDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDE
                     NSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDSHLSSICT
                     PADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAERVRNGLKFA
                     GVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLDSSGGKKLLG
                     LSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGELLEGSGQTVVP
                     DGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFLSQDLIGTEAHM
                     DSSSDTKEL"
     CDS             3157..3186
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             3202..3219
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      3299..3545
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        3560..3971
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        3979..4026
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             4045..4416
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      4485..4732
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(4807..5395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
     1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt
     1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac
     1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct
     1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac
     1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct
     1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc
     1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg
     1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa
     1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca
     1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac
     1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat
     1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg
     1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg
     1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt
     2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg
     2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg
     2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa
     2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg
     2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt
     2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg
     2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc
     2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg
     2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt
     2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat
     2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag
     2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa
     2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg
     2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg
     2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt
     2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt
     3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg
     3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac
     3121 atggacagca gcagcgacac gaaggaattg ggtctagaac aaaaactcat ctcagaagag
     3181 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttgtag ccttagacat
     3241 gactgttcct cagttcaagt tgggcactta cgagaagacc ggtcttgcta gattctaatc
     3301 aagaggatgt cagaatgcca tttgcctgag agatgcaggc ttcatttttg atactttttt
     3361 atttgtaacc tatatagtat aggatttttt ttgtcatttt gtttcttctc gtacgagctt
     3421 gctcctgatc agcctatctc gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc
     3481 attcgagttt gatgtttttc ttggtatttc ccactcctct tcagagtaca gaagattaag
     3541 tgagaccttc gtttgtgcgg atcccccaca caccatagct tcaaaatgtt tctactcctt
     3601 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa
     3661 gcacagcata ctaaattttc cctctttctt cctctagggt gtcgttaatt acccgtacta
     3721 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat
     3781 aaaaattttt atcacgtttc tttttcttga aatttttttt tttagttttt ttctctttca
     3841 gtgacctcca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca
     3901 tttttcttgt tctattacaa ctttttttac ttcttgttca ttagaaagaa agcatagcaa
     3961 tctaatctaa ggggcggtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     4021 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     4081 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     4141 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     4201 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     4261 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     4321 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     4381 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg gcggcccacg
     4441 ggtcccaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt aattagttat
     4501 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag
     4561 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt
     4621 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat
     4681 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctgga
     4741 gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4801 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4861 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4921 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4981 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5041 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5101 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5161 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5221 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5281 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5341 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5401 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5461 tttggtcatg agatc
//
