LOCUS       Exported                3329 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             582..611
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             627..644
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      724..970
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        991..1398
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1405..1452
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1471..1845
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1911..2158
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2233..2821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        join(2903..3329,1..512)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
ORIGIN
        1 acctaatatg acaaaagcgt gatctcatcc aagatgaact aagtttggtt cgttgaaatg
       61 ctaacggcca gttggtcaaa aagaaacttc caaaagtcgg cataccgttt gtcttgtttg
      121 gtattgattg acgaatgctc aaaaataatc tcattaatgc ttagcgcagt ctctctatcg
      181 cttctgaacc ccggtgcacc tgtgccgaaa cgcaaatggg gaaacacccg ctttttggat
      241 gattatgcat tgtctccaca ttgtatgctt ccaagattct ggtgggaata ctgctgatag
      301 cctaacgttc atgatcaaaa tttaactgtt ctaaccccta cttgacagca atatataaac
      361 agaaggaagc tgccctgtct taaacctttt tttttatcat cattattagc ttactttcat
      421 aattgcgact ggttccaatt gacaagcttt tgattttaac gacttttaac gacaacttga
      481 gaagatcaaa aaacaactaa ttattcgaaa cgaggaattc acgtggccca gccggccgtc
      541 tcggatcggt acctcgagcc gcggcggccg ccagctttct agaacaaaaa ctcatctcag
      601 aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt tgtagcctta
      661 gacatgactg ttcctcagtt caagttgggc acttacgaga agaccggtct tgctagattc
      721 taatcaagag gatgtcagaa tgccatttgc ctgagagatg caggcttcat ttttgatact
      781 tttttatttg taacctatat agtataggat tttttttgtc attttgtttc ttctcgtacg
      841 agcttgctcc tgatcagcct atctcgcagc tgatgaatat cttgtggtag gggtttggga
      901 aaatcattcg agtttgatgt ttttcttggt atttcccact cctcttcaga gtacagaaga
      961 ttaagtgaga ccttcgtttg tgcggatccc ccacacacca tagcttcaaa atgtttctac
     1021 tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca
     1081 cccaagcaca gcatactaaa tttcccctct ttcttcctct agggtgtcgt taattacccg
     1141 tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag
     1201 gcaataaaaa tttttatcac gtttcttttt cttgaaaatt tttttttttg atttttttct
     1261 ctttcgatga cctcccattg atatttaagt taataaacgg tcttcaattt ctcaagtttc
     1321 agtttcattt ttcttgttct attacaactt tttttacttc ttgctcatta gaaagaaagc
     1381 atagcaatct aatctaaggg cggtgttgac aattaatcat cggcatagta tatcggcata
     1441 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     1501 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     1561 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     1621 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     1681 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     1741 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     1801 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt ccgacgcggc
     1861 ccgacgggtc cgaggcctcg gagatccgtc ccccttttcc tttgtcgata tcatgtaatt
     1921 agttatgtca cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg
     1981 agttagacaa cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag
     2041 aacgttattt atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa
     2101 cattatactg aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcaa
     2161 gctggagacc aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2881 agggattttg gtcatgagat cagatctaac atccaaagac gaaaggttga atgaaacctt
     2941 tttgccatcc gacatccaca ggtccattct cacacataag tgccaaacgc aacaggaggg
     3001 gatacactag cagcagaccg ttgcaaacgc aggacctcca ctcctcttct cctcaacacc
     3061 cacttttgcc atcgaaaaac cagcccagtt attgggcttg attggagctc gctcattcca
     3121 attccttcta ttaggctact aacaccatga ctttattagc ctgtctatcc tggcccccct
     3181 ggcgaggttc atgtttgttt atttccgaat gcaacaagct ccgcattaca cccgaacatc
     3241 actccagatg agggctttct gagtgtgggg tcaaatagtt tcatgttccc caaatggccc
     3301 aaaactgaca gtttaaacgc tgtcttgga
//
