LOCUS       62056_18505        4198 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             1881..1910
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1926..1943
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2022..2268
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2283..2694
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2702..2749
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2768..3139
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3208..3455
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3530..4118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggcccagc
      961 cggccgtctc ggatcggtac ctcgagatgt cgaaagcctc cattgggtta tttgtgccag
     1021 caagtcctcc tctggaccct gagaaggtca aagagttaca gcgcttcatc accctttcca
     1081 agagactcct tgtgatgact ggggcaggaa tctccaccga atcggggata ccagactaca
     1141 ggtcagaaaa agtggggctt tatgcccgca ctgaccgcag gcccatccag catggtgatt
     1201 ttgtccggag tgccccaatc cgccagcggt actgggcgag aaacttcgta ggctggcctc
     1261 aattctcctc ccaccagcct aaccctgcac actgggcttt gagcacctgg gagaaactcg
     1321 gaaagctgta ctggttggtg acccaaaatg tggatgcttt gcacaccaag gcggggagtc
     1381 ggcgcctgac agagctccac ggatgcatgg acagggtcct gtgcttggat tgtggggaac
     1441 agactccccg gggggtgctg caagagcgtt tccaagtcct gaaccccacc tggagtgctg
     1501 aggcccatgg cctggctcct gatggtgacg tctttctctc agaggagcaa gtccggagct
     1561 ttcaggtccc aacctgcgtt caatgtggag gccatctgaa accagatgtc gttttcttcg
     1621 gggacacagt gaaccctgac aaggttgatt ttgtgcacaa gcgtgtaaaa gaagccgact
     1681 ccctcttggt ggtgggatca tccttgcagg tatactctgg ttacaggttt atcctcactg
     1741 cctgggagaa gaagctcccg attgcaatac tgaacattgg gcccacacgg tcggatgact
     1801 tggcgtgtct gaaactgaat tctcgttgtg gagagttgct gcctttgata gacccatgca
     1861 gcggccgcca gcttgggccc gaacaaaaac tcatctcaga agaggatctg aatagcgccg
     1921 tcgaccatca tcatcatcat cattgagttt tagccttaga catgactgtt cctcagttca
     1981 agttgggcac ttacgagaag accggtcttg ctagattcta atcaagagga tgtcagaatg
     2041 ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta acctatatag
     2101 tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg atcagcctat
     2161 ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag tttgatgttt
     2221 ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagacc ttcgtttgtg
     2281 cggatccccc acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt
     2341 ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt
     2401 ttccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa
     2461 aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt
     2521 ttctttttct tgaaattttt ttttttagtt tttttctctt tcagtgacct ccattgatat
     2581 ttaagttaat aaacggtctt caatttctca agtttcagtt tcatttttct tgttctatta
     2641 caactttttt tacttcttgt tcattagaaa gaaagcatag caatctaatc taaggggcgg
     2701 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     2761 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     2821 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     2881 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     2941 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     3001 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     3061 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     3121 cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca ggcctcggag
     3181 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
     3241 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     3301 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     3361 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     3421 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa
     3481 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3541 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3601 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3661 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3721 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3781 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3841 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3901 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3961 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4021 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4081 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4141 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagatc
//
