LOCUS       Exported                3330 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             480..509
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             525..542
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      622..868
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        889..1296
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1303..1350
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1369..1743
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1809..2056
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2131..2719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        join(2801..3330,1..409)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
ORIGIN
        1 accgtttgtc ttgtttggta ttgattgacg aatgctcaaa aataatctca ttaatgctta
       61 gcgcagtctc tctatcgctt ctgaaccccg gtgcacctgt gccgaaacgc aaatggggaa
      121 acacccgctt tttggatgat tatgcattgt ctccacattg tatgcttcca agattctggt
      181 gggaatactg ctgatagcct aacgttcatg atcaaaattt aactgttcta acccctactt
      241 gacagcaata tataaacaga aggaagctgc cctgtcttaa accttttttt ttatcatcat
      301 tattagctta ctttcataat tgcgactggt tccaattgac aagcttttga ttttaacgac
      361 ttttaacgac aacttgagaa gatcaaaaaa caactaatta ttcgaaacga ggaattcacg
      421 tggcccagcc ggccgtctcg gatcggtacc tcgagccgcg gcggccgcca gcttacgtag
      481 aacaaaaact catctcagaa gaggatctga atagcgccgt cgaccatcat catcatcatc
      541 attgagtttg tagccttaga catgactgtt cctcagttca agttgggcac ttacgagaag
      601 accggtcttg ctagattcta atcaagagga tgtcagaatg ccatttgcct gagagatgca
      661 ggcttcattt ttgatacttt tttatttgta acctatatag tataggattt tttttgtcat
      721 tttgtttctt ctcgtacgag cttgctcctg atcagcctat ctcgcagctg atgaatatct
      781 tgtggtaggg gtttgggaaa atcattcgag tttgatgttt ttcttggtat ttcccactcc
      841 tcttcagagt acagaagatt aagtgagacc ttcgtttgtg cggatccccc acacaccata
      901 gcttcaaaat gtttctactc cttttttact cttccagatt ttctcggact ccgcgcatcg
      961 ccgtaccact tcaaaacacc caagcacagc atactaaatt tcccctcttt cttcctctag
     1021 ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa aaagagaccg cctcgtttct
     1081 ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt ttctttttct tgaaaatttt
     1141 tttttttgat ttttttctct ttcgatgacc tcccattgat atttaagtta ataaacggtc
     1201 ttcaatttct caagtttcag tttcattttt cttgttctat tacaactttt tttacttctt
     1261 gctcattaga aagaaagcat agcaatctaa tctaagggcg gtgttgacaa ttaatcatcg
     1321 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     1381 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     1441 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     1501 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     1561 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     1621 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     1681 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     1741 tgacacgtcc gacgcggccc gacgggtccg aggcctcgga gatccgtccc ccttttcctt
     1801 tgtcgatatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
     1861 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
     1921 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
     1981 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
     2041 gaaggcttta atttgcaagc tggagaccaa catgtgagca aaaggccagc aaaaggccag
     2101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     2161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     2221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2761 gaacgaaaac tcacgttaag ggattttggt catgagatca gatctaacat ccaaagacga
     2821 aaggttgaat gaaacctttt tgccatccga catccacagg tccattctca cacataagtg
     2881 ccaaacgcaa caggagggga tacactagca gcagaccgtt gcaaacgcag gacctccact
     2941 cctcttctcc tcaacaccca cttttgccat cgaaaaacca gcccagttat tgggcttgat
     3001 tggagctcgc tcattccaat tccttctatt aggctactaa caccatgact ttattagcct
     3061 gtctatcctg gcccccctgg cgaggttcat gtttgtttat ttccgaatgc aacaagctcc
     3121 gcattacacc cgaacatcac tccagatgag ggctttctga gtgtggggtc aaatagtttc
     3181 atgttcccca aatggcccaa aactgacagt ttaaacgctg tcttggaacc taatatgaca
     3241 aaagcgtgat ctcatccaag atgaactaag tttggttcgt tgaaatgcta acggccagtt
     3301 ggtcaaaaag aaacttccaa aagtcggcat
//
