LOCUS       62056_18490        4459 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /codon_start=1
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     CDS             2141..2170
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2186..2203
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2283..2529
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        2544..2955
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2963..3010
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3029..3400
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      3469..3716
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3791..4379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgaa ttcacgtggc ccagccggcc gtctcggatc ggtacctcga gccgcggcgg
     1261 ccgctcgaaa gcctccattg ggttatttgt gccagcaagt cctcctctgg accctgagaa
     1321 ggtcaaagag ttacagcgct tcatcaccct ttccaagaga ctccttgtga tgactggggc
     1381 aggaatctcc accgaatcgg ggataccaga ctacaggtca gaaaaagtgg ggctttatgc
     1441 ccgcactgac cgcaggccca tccagcatgg tgattttgtc cggagtgccc caatccgcca
     1501 gcggtactgg gcgagaaact tcgtaggctg gcctcaattc tcctcccacc agcctaaccc
     1561 tgcacactgg gctttgagca cctgggagaa actcggaaag ctgtactggt tggtgaccca
     1621 aaatgtggat gctttgcaca ccaaggcggg gagtcggcgc ctgacagagc tccacggatg
     1681 catggacagg gtcctgtgct tggattgtgg ggaacagact ccccgggggg tgctgcaaga
     1741 gcgtttccaa gtcctgaacc ccacctggag tgctgaggcc catggcctgg ctcctgatgg
     1801 tgacgtcttt ctctcagagg agcaagtccg gagctttcag gtcccaacct gcgttcaatg
     1861 tggaggccat ctgaaaccag atgtcgtttt cttcggggac acagtgaacc ctgacaaggt
     1921 tgattttgtg cacaagcgtg taaaagaagc cgactccctc ttggtggtgg gatcatcctt
     1981 gcaggtatac tctggttaca ggtttatcct cactgcctgg gagaagaagc tcccgattgc
     2041 aatactgaac attgggccca cacggtcgga tgacttggcg tgtctgaaac tgaattctcg
     2101 ttgtggagag ttgctgcctt tgatagaccc atgccctcta gaacaaaaac tcatctcaga
     2161 agaggatctg aatagcgccg tcgaccatca tcatcatcat cattgagttt gtagccttag
     2221 acatgactgt tcctcagttc aagttgggca cttacgagaa gaccggtctt gctagattct
     2281 aatcaagagg atgtcagaat gccatttgcc tgagagatgc aggcttcatt tttgatactt
     2341 ttttatttgt aacctatata gtataggatt ttttttgtca ttttgtttct tctcgtacga
     2401 gcttgctcct gatcagccta tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa
     2461 aatcattcga gtttgatgtt tttcttggta tttcccactc ctcttcagag tacagaagat
     2521 taagtgagac cttcgtttgt gcggatcccc cacacaccat agcttcaaaa tgtttctact
     2581 ccttttttac tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac
     2641 ccaagcacag catactaaat tttccctctt tcttcctcta gggtgtcgtt aattacccgt
     2701 actaaaggtt tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg
     2761 caataaaaat ttttatcacg tttctttttc ttgaaatttt tttttttagt ttttttctct
     2821 ttcagtgacc tccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt
     2881 ttcatttttc ttgttctatt acaacttttt ttacttcttg ttcattagaa agaaagcata
     2941 gcaatctaat ctaaggggcg gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     3001 ataatacgac aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct
     3061 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     3121 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     3181 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     3241 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     3301 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     3361 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtcc gacggcggcc
     3421 cacgggtccc aggcctcgga gatccgtccc ccttttcctt tgtcgatatc atgtaattag
     3481 ttatgtcacg cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag
     3541 ttagacaacc tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa
     3601 cgttatttat atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca
     3661 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagc
     3721 tggagaccaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3781 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3841 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3901 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3961 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     4021 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4081 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4141 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4201 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     4261 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4321 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4381 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4441 ggattttggt catgagatc
//
