LOCUS       40924_34540       10878 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast expression vector pPIC9-TSE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10878)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 10878)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10878)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..937
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             949..1215
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     CDS             1219..3141
                     /codon_start=1
                     /note="unnamed protein product; mature TS"
                     /protein_id="SJL88089.1"
                     /translation="MLAPGSSRVELFKRKNSTVPFEDKAGKVTERVVHSFRLPALVNVD
                     GVMVAIADARYDTSNDNSLIDTVAKYSVDDGETWETQIAIKNSRVSSVSRVVDPTVIVK
                     GNKLYVLVGSYYSSRSYWSSHGDARDWDILLAVGEVTKSIAGGKITASIKWGSPVSLKK
                     FFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKRKQVFSKIFYSEDDGKTWKFG
                     KGRSDFGCSEPVALEWEGKLIINTRVDWKRRLVYESSDMGNTWVEAVGTLSRVWGPSPK
                     SDHPGSQSSFTAVTIEGMRVMLFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDE
                     NSAYSSVLYKDDKLYCLHEINTDEVYSLVFARLVGELRIIKSVLRSWKNWDSHLSSICT
                     PADPAASSSESGCGPAVTTVGLVGFLSGNASQNVWEDAYRCVNASTANAERVRNGLKFA
                     GVGGGALWPVSQQGQNQRYRFANHAFTLVASVTIHEAPRAASPLLGASLDSSGGKKLLG
                     LSYDEKHQWQPIYGSTPVTPTGSWETGKRYHVVLTVANKIGSVYIDGELLEGSGQTVVP
                     DGRTPDISHFYVGGYGRSDMPTISHVTVNNVLLYNRQLNTEEIRTLFLSQDLIGTEAHM
                     DSSSDT"
     misc_feature    3151..3189
                     /label=E-tag
                     /note="E-tag"
     CDS             3199..4090
                     /codon_start=1
                     /note="unnamed protein product; fd g3 fragment"
                     /protein_id="SJL88090.1"
                     /translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
                     DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
                     YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
                     SGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENALQSD
                     AKGKLDSVATDYGAAI"
     terminator      4176..4422
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(4835..7369)
                     /codon_start=1
                     /gene="Pichia pastoris HIS4"
                     /product="multifunctional enzyme, required for histidine 
                     biosynthesis"
                     /label=PpHIS4
                     /note="auxotrophic marker for Pichia pastoris"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     CDS             complement(4838..7369)
                     /codon_start=1
                     /note="unnamed protein product; Pichia HIS4"
                     /protein_id="SJL88091.1"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     misc_feature    7724..8480
                     /label=AOX1 3' fragment
                     /note="region downstream of Pichia pastoris AOX1 gene"
     misc_feature    8623..8763
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8949..9537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9711..10568)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10569..10673)
                     /label=AmpR promoter
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct
      961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact
     1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta
     1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg
     1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa
     1201 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt
     1261 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac
     1321 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct
     1381 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac
     1441 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct
     1501 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc
     1561 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg
     1621 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa
     1681 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca
     1741 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac
     1801 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat
     1861 gatgggaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg
     1921 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg
     1981 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt
     2041 gtgtggggcc cctcaccaaa atcggaccat cccggcagtc agagcagctt cactgccgtg
     2101 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg
     2161 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa
     2221 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg
     2281 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt
     2341 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg
     2401 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc
     2461 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg
     2521 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt
     2581 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat
     2641 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag
     2701 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa
     2761 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg
     2821 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg
     2881 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt
     2941 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt
     3001 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg
     3061 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac
     3121 atggacagca gcagcgacac gaaggaattg ggtgcgccgg tgccgtatcc ggatccgctg
     3181 gaaccgcgtg ccgcatagac tgttgaaagt tgtttagcaa aacctcatac agaaaattca
     3241 tttactaacg tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggctgt
     3301 ctgtggaatg ctacaggcgt tgtggtttgt actggtgacg aaactcagtg ttacggtaca
     3361 tgggttccta ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt
     3421 tctgagggtg gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct
     3481 attccgggct atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa
     3541 aaccccgcta atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt
     3601 cagaataata ggttccgaaa taggcagggt gcattaactg tttatacggg cactgttact
     3661 caaggcactg accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg
     3721 tatgacgctt actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgag
     3781 gatccattcg tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat
     3841 gctggcggcg gctctggtgg tggttctggt ggcggctctg agggtggcgg ctctgagggt
     3901 ggcggttctg agggtggcgg ctctgagggt ggcggttccg gtggcggctc cggttccggt
     3961 gattttgatt atgaaaaaat ggcaaacgct aataaggggg ctatgaccga aaatgccgat
     4021 gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt
     4081 gctgctatcg ggccgcgaat taattcgcct tagacatgac tgttcctcag ttcaagttgg
     4141 gcacttacga gaagaccggt cttgctagat tctaatcaag aggatgtcag aatgccattt
     4201 gcctgagaga tgcaggcttc atttttgata cttttttatt tgtaacctat atagtatagg
     4261 attttttttg tcattttgtt tcttctcgta cgagcttgct cctgatcagc ctatctcgca
     4321 gctgatgaat atcttgtggt aggggtttgg gaaaatcatt cgagtttgat gtttttcttg
     4381 gtatttccca ctcctcttca gagtacagaa gattaagtga gaagttcgtt tgtgcaagct
     4441 tatcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc
     4501 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt
     4561 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga
     4621 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg
     4681 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc
     4741 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcta
     4801 tcgaatctaa atgtaagtta aaatctctaa ataattaaat aagtcccagt ttctccatac
     4861 gaaccttaac agcattgcgg tgagcatcta gaccttcaac agcagccaga tccatcactg
     4921 cttggccaat atgtttcagt ccctcaggag ttacgtcttg tgaagtgatg aacttctgga
     4981 aggttgcagt gttaactccg ctgtattgac gggcatatcc gtacgttggc aaagtgtggt
     5041 tggtaccgga ggagtaatct ccacaactct ctggagagta ggcaccaaca aacacagatc
     5101 cagcgtgttg tacttgatca acataagaag aagcattctc gatttgcagg atcaagtgtt
     5161 caggagcgta ctgattggac atttccaaag cctgctcgta ggttgcaacc gatagggttg
     5221 tagagtgtgc aatacacttg cgtacaattt caacccttgg caactgcaca gcttggttgt
     5281 gaacagcatc ttcaattctg gcaagctcct tgtctgtcat atcgacagcc aacagaatca
     5341 cctgggaatc aataccatgt tcagcttgag acagaaggtc tgaggcaacg aaatctggat
     5401 cagcgtattt atcagcaata actagaactt cagaaggccc agcaggcatg tcaatactac
     5461 acagggctga tgtgtcattt tgaaccatca tcttggcagc agtaacgaac tggtttcctg
     5521 gaccaaatat tttgtcacac ttaggaacag tttctgttcc gtaagccata gcagctactg
     5581 cctgggcgcc tcctgctagc acgatacact tagcaccaac cttgtgggca acgtagatga
     5641 cttctggggt aagggtacca tccttcttag gtggagatgc aaaaacaatt tctttgcaac
     5701 cagcaacttt ggcaggaaca cccagcatca gggaagtgga aggcagaatt gcggttccac
     5761 caggaatata gaggccaact ttctcaatag gtcttgcaaa acgagagcag actacaccag
     5821 ggcaagtctc aacttgcaac gtctccgtta gttgagcttc atggaatttc ctgacgttat
     5881 ctatagagag atcaatggct ctcttaacgt tatctggcaa ttgcataagt tcctctggga
     5941 aaggagcttc taacacaggt gtcttcaaag cgactccatc aaacttggca gttagttcta
     6001 aaagggcttt gtcaccattt tgacgaacat tgtcgacaat tggtttgact aattccataa
     6061 tctgttccgt tttctggata ggacgacgaa gggcatcttc aatttcttgt gaggaggcct
     6121 tagaaacgtc aattttgcac aattcaatac gaccttcaga agggacttct ttaggtttgg
     6181 attcttcttt aggttgttcc ttggtgtatc ctggcttggc atctcctttc cttctagtga
     6241 cctttaggga cttcatatcc aggtttctct ccacctcgtc caacgtcaca ccgtacttgg
     6301 cacatctaac taatgcaaaa taaaataagt cagcacattc ccaggctata tcttccttgg
     6361 atttagcttc tgcaagttca tcagcttcct ccctaatttt agcgttcaac aaaacttcgt
     6421 cgtcaaataa ccgtttggta taagaacctt ctggagcatt gctcttacga tcccacaagg
     6481 tggcttccat ggctctaaga ccctttgatt ggccaaaaca ggaagtgcgt tccaagtgac
     6541 agaaaccaac acctgtttgt tcaaccacaa atttcaagca gtctccatca caatccaatt
     6601 cgatacccag caacttttga gttgctccag atgtagcacc tttataccac aaaccgtgac
     6661 gacgagattg gtagactcca gtttgtgtcc ttatagcctc cggaatagac tttttggacg
     6721 agtacaccag gcccaacgag taattagaag agtcagccac caaagtagtg aatagaccat
     6781 cggggcggtc agtagtcaaa gacgccaaca aaatttcact gacagggaac tttttgacat
     6841 cttcagaaag ttcgtattca gtagtcaatt gccgagcatc aataatgggg attataccag
     6901 aagcaacagt ggaagtcaca tctaccaact ttgcggtctc agaaaaagca taaacagttc
     6961 tactaccgcc attagtgaaa cttttcaaat cgcccagtgg agaagaaaaa ggcacagcga
     7021 tactagcatt agcgggcaag gatgcaactt tatcaaccag ggtcctatag ataaccctag
     7081 cgcctgggat catcctttgg acaactcttt ctgccaaatc taggtccaaa atcacttcat
     7141 tgataccatt attgtacaac ttgagcaagt tgtcgatcag ctcctcaaat tggtcctctg
     7201 taacggatga ctcaacttgc acattaactt gaagctcagt cgattgagtg aacttgatca
     7261 ggttgtgcag ctggtcagca gcatagggaa acacggcttt tcctaccaaa ctcaaggaat
     7321 tatcaaactc tgcaacactt gcgtatgcag gtagcaaggg aaatgtcata cttgaagtcg
     7381 gacagtgagt gtagtcttga gaaattctga agccgtattt ttattatcag tgagtcagtc
     7441 atcaggagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     7501 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     7561 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     7621 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     7681 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgagta tctatgattg
     7741 gaagtatggg aatggtgata cccgcattct tcagtgtctt gaggtctcct atcagattat
     7801 gcccaactaa agcaaccgga ggaggagatt tcatggtaaa tttctctgac ttttggtcat
     7861 cagtagactc gaactgtgag actatctcgg ttatgacagc agaaatgtcc ttcttggaga
     7921 cagtaaatga agtcccacca ataaagaaat ccttgttatc aggaacaaac ttcttgtttc
     7981 gaactttttc ggtgccttga actataaaat gtagagtgga tatgtcgggt aggaatggag
     8041 cgggcaaatg cttaccttct ggaccttcaa gaggtatgta gggtttgtag atactgatgc
     8101 caacttcagt gacaacgttg ctatttcgtt caaaccattc cgaatccaga gaaatcaaag
     8161 ttgtttgtct actattgatc caagccagtg cggtcttgaa actgacaata gtgtgctcgt
     8221 gttttgaggt catctttgta tgaataaatc tagtctttga tctaaataat cttgacgagc
     8281 caaggcgata aatacccaaa tctaaaactc ttttaaaacg ttaaaaggac aagtatgtct
     8341 gcctgtatta aaccccaaat cagctcgtag tctgatcctc atcaacttga ggggcactat
     8401 cttgttttag agaaatttgc ggagatgcga tatcgagaaa aaggtacgct gattttaaac
     8461 gtgaaattta tctcaagatc tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
     8521 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8581 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
     8641 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
     8701 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     8761 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9121 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc
     9181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     9421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10021 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10321 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10681 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
    10741 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttctcatgtt tgacagctta
    10801 tcatcgataa gctgactcat gttggtattg tgaaatagac gcagatcggg aacactgaaa
    10861 aataacagtt attattcg
//
