LOCUS       pPIC6-C.        3651 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein. For other reading frames, use pPIC6-alpha A or 
            pPIC6-alpha B.
ACCESSION   .
VERSION     .
KEYWORDS    pPIC6-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3651)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3651)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1207..1281
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1280..1309
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1325..1342
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1422..1668
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1683..2096
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2103..2150
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2169..2564
                     /label=BSD
                     /note="blasticidin S deaminase"
     terminator      2661..2908
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2983..3571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga agagagaggc
     1201 tgaagcatcg atgaattcac gtggcccagc cggccgtctc ggatcggtac ctcgagccgc
     1261 ggcggccgcc agctttctag aacaaaaact catctcagaa gaggatctga atagcgccgt
     1321 cgaccatcat catcatcatc attgagtttg tagccttaga catgactgtt cctcagttca
     1381 agttgggcac ttacgagaag accggtcttg ctagattcta atcaagagga tgtcagaatg
     1441 ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta acctatatag
     1501 tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg atcagcctat
     1561 ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag tttgatgttt
     1621 ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagacc ttcgtttgtg
     1681 cggatccccc acacaccata gcttcaaaat gtttctactc cttttttact cttccagatt
     1741 ttctcggact ccgcgcatcg ccgtaccact tcaaaacacc caagcacagc atactaaatt
     1801 tcccctcttt cttcctctag ggtgtcgtta attacccgta ctaaaggttt ggaaaagaaa
     1861 aaagagaccg cctcgtttct ttttcttcgt cgaaaaaggc aataaaaatt tttatcacgt
     1921 ttctttttct tgaaaatttt tttttttgat ttttttctct ttcgatgacc tcccattgat
     1981 atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat
     2041 tacaactttt tttacttctt gttcattaga aagaaagcat agcaatctaa tctaagggcg
     2101 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     2161 actaaaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     2221 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     2281 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     2341 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     2401 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     2461 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     2521 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaagca cttcgtggcc
     2581 gaggagcagg actgacacgt ccgacgcggc ccgacgggtc ccaggcctcg gagatccgtc
     2641 ccccttttcc tttgtcgata tcatgtaatt agttatgtca cgcttacatt cacgccctcc
     2701 ccccacatcc gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat
     2761 ttattttttt atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt
     2821 ttttctgtac agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt
     2881 tttgggacgc tcgaaggctt taatttgcaa gctggagacc aacatgtgag caaaaggcca
     2941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3421 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat c
//
