LOCUS       pPIC6-A.        3646 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for methanol-inducible expression of a 
            secreted protein. For other reading frames, use pPIC6-alpha B or 
            pPIC6-alpha C.
ACCESSION   .
VERSION     .
KEYWORDS    pPIC6-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3646)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3646)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize with SacI, PmeI, or BstXI for integration at the AOX1 
            locus.
FEATURES             Location/Qualifiers
     source          1..3646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             941..1207
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    1208..1276
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             1275..1304
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1320..1337
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      1417..1663
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1678..2091
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2098..2145
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2164..2559
                     /label=BSD
                     /note="blasticidin S deaminase"
     terminator      2656..2903
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2978..3566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg atgagatttc cttcaatttt
      961 tactgctgtt ttattcgcag catcctccgc attagctgct ccagtcaaca ctacaacaga
     1021 agatgaaacg gcacaaattc cggctgaagc tgtcatcggt tactcagatt tagaagggga
     1081 tttcgatgtt gctgttttgc cattttccaa cagcacaaat aacgggttat tgtttataaa
     1141 tactactatt gccagcattg ctgctaaaga agaaggggta tctctcgaga aaagagaggc
     1201 tgaagctgaa ttcacgtggc ccagccggcc gtctcggatc ggtacctcga gccgcggcgg
     1261 ccgccagctt tctagaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc
     1321 atcatcatca tcatcattga gtttgtagcc ttagacatga ctgttcctca gttcaagttg
     1381 ggcacttacg agaagaccgg tcttgctaga ttctaatcaa gaggatgtca gaatgccatt
     1441 tgcctgagag atgcaggctt catttttgat acttttttat ttgtaaccta tatagtatag
     1501 gatttttttt gtcattttgt ttcttctcgt acgagcttgc tcctgatcag cctatctcgc
     1561 agctgatgaa tatcttgtgg taggggtttg ggaaaatcat tcgagtttga tgtttttctt
     1621 ggtatttccc actcctcttc agagtacaga agattaagtg agaccttcgt ttgtgcggat
     1681 cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
     1741 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc
     1801 tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga
     1861 gaccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt
     1921 tttcttgaaa attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta
     1981 agttaataaa cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa
     2041 ctttttttac ttcttgttca ttagaaagaa agcatagcaa tctaatctaa gggcggtgtt
     2101 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     2161 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     2221 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     2281 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     2341 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     2401 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     2461 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     2521 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga
     2581 gcaggactga cacgtccgac gcggcccgac gggtcccagg cctcggagat ccgtccccct
     2641 tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc cctcccccca
     2701 catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt
     2761 tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc
     2821 tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg
     2881 gacgctcgaa ggctttaatt tgcaagctgg agaccaacat gtgagcaaaa ggccagcaaa
     2941 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3001 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3061 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3121 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3181 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3241 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3301 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3361 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     3421 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3481 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3541 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3601 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagatc
//
