LOCUS       40924_34510        3382 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..940
                     /label=AOX1 promoter
                     /note="inducible promoter, regulated by methanol"
     CDS             1012..1041
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1057..1074
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1154..1400
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1415..1826
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1834..1881
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1900..2295
                     /codon_start=1
                     /label=BSD
                     /note="blasticidin S deaminase"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     terminator      2392..2639
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2714..3302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag
       61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt
      121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc
      181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta
      241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta
      301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg
      361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct
      421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg
      481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcggca taccgtttgt
      541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct
      601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct
      661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact
      721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat
      781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt
      841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga
      901 caacttgaga agatcaaaaa acaactaatt attcgaaacg aggaattcac gtggcccagc
      961 cggccgtctc ggatcggtac ctcgagccgc ggcggccgcc agcttgggcc cgaacaaaaa
     1021 ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt
     1081 tgtagcctta gacatgactg ttcctcagtt caagttgggc acttacgaga agaccggtct
     1141 tgctagattc taatcaagag gatgtcagaa tgccatttgc ctgagagatg caggcttcat
     1201 ttttgatact tttttatttg taacctatat agtataggat tttttttgtc attttgtttc
     1261 ttctcgtacg agcttgctcc tgatcagcct atctcgcagc tgatgaatat cttgtggtag
     1321 gggtttggga aaatcattcg agtttgatgt ttttcttggt atttcccact cctcttcaga
     1381 gtacagaaga ttaagtgaga ccttcgtttg tgcggatccc ccacacacca tagcttcaaa
     1441 atgtttctac tcctttttta ctcttccaga ttttctcgga ctccgcgcat cgccgtacca
     1501 cttcaaaaca cccaagcaca gcatactaaa ttttccctct ttcttcctct agggtgtcgt
     1561 taattacccg tactaaaggt ttggaaaaga aaaaagagac cgcctcgttt ctttttcttc
     1621 gtcgaaaaag gcaataaaaa tttttatcac gtttcttttt cttgaaattt ttttttttag
     1681 tttttttctc tttcagtgac ctccattgat atttaagtta ataaacggtc ttcaatttct
     1741 caagtttcag tttcattttt cttgttctat tacaactttt tttacttctt gttcattaga
     1801 aagaaagcat agcaatctaa tctaaggggc ggtgttgaca attaatcatc ggcatagtat
     1861 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa
     1921 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa
     1981 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat
     2041 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct
     2101 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg
     2161 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata
     2221 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt
     2281 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tccgacggcg
     2341 gcccacgggt cccaggcctc ggagatccgt cccccttttc ctttgtcgat atcatgtaat
     2401 tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc gaaaaggaag
     2461 gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg ttagtattaa
     2521 gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg tacgcatgta
     2581 acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct ttaatttgca
     2641 agctggagac caacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2881 tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt
     2941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3121 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     3181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3361 aagggatttt ggtcatgaga tc
//
