LOCUS       40924_34480        4471 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transformation vector pPIBT7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Transformation of Ulva mutabilis Foyn with plasmids integrating into
            the chromosome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Oertel W, Weissgerber A.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-SEP-2007) Biochemistry, Genetics and Microbiology, 
            Division of Genetics, University of Regensburg, Universitaetsstr. 
            31, Regensburg, Bavaria 93053, Germany
REFERENCE   3  (bases 1 to 4471)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4471)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-SEP-2007) Biochemistry, Genetics and Microbiology, Division of 
            Genetics, University of Regensburg, Universitaetsstr. 31, 
            Regensburg, Bavaria 93053, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..546
                     /label=Ulva mutabilis RbcS promoter
                     /note="Ulva mutabilis RbcS promoter"
                     /regulatory_class="promoter"
     intron          555..1051
                     /gene="ble"
                     /note="Ulva mutabilis RbcS intron 1; enhancer"
     CDS             1050..1415
                     /label=BleoR
                     /note="antibiotic-binding protein"
     regulatory      1419..1807
                     /label=Ulva mutabilis RbcS terminator
                     /note="Ulva mutabilis RbcS terminator"
                     /regulatory_class="terminator"
     primer_bind     complement(1834..1850)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2324..2428
                     /label=AmpR promoter
     CDS             2429..3286
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3460..4048
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4336..4357
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4372..4402
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4410..4426
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4434..4450
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgttgttcgt acacaaggat gtcgcttgtg gctttttcgc tcggctttcc aggagggcag
       61 tccagatacc atgcggccgc cgtgtccatc actcgcgctg cagctgatgt gggcagatac
      121 tgcactaatc tgccgcacga cgcgtcggcc tgagtcggcg gacaccggag gcttgctgga
      181 gtactggcac aagtgtgcgt gtgattgcca gaaacagcaa cccactcact agatataccg
      241 ctccaataat gggttcatgt gcaagctcac tcaataacga aacgcttcac tattgtgcac
      301 gtgccggggt tgcttgggta tggcgtgcca tacgggttct gcacatgtca cccacgtggg
      361 tgtggcacga gatatcgagg tggccatgta aaaggcattt gctagagggc agctcgtcca
      421 tgccgctcga gcacaccgag tgcgcaccat aaatgcagca catgcctgtg cagcctatct
      481 cctgtctctg ttacatccca ccacctcctc acatatcccc ttccaccctt aaaccgcaca
      541 gcagacatgg ccaagtgcgc atcgcgtgtc aatgctggcg ctgctgacgt ccgctgccta
      601 aactgcatga aagctcctgc agtcgcaagt agtcctttta acttgttgta tcggaacaaa
      661 tggttgtagt ctgtgccgtg tgacgatctt gagccgcctg gtcggcaggt ccaatgtcgg
      721 cctggaccct gcacaccctg cccaccctgc cgctgctcac ccgttcacga ccccacaggt
      781 gctcgcagca gtaattgtcg atagatagca actgtgttct gactattctt tgaccttccg
      841 catttgggac tgcggcggca cacctactat ccgcaatgca caccctggtg ctggtactgc
      901 ctgcctttcc gcgcgagccc acaccatgca ggcgcatagt aatggactgt gattgattta
      961 ggcaactgta tgaaacctct tcatcgtgcc ttggttgctt gcgataccct cacgtgagtt
     1021 catatgacca acgctggcac gacccctgca ggatcaccag cgccgttccg gtgctcaccg
     1081 cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact
     1141 tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg
     1201 tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg
     1261 agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg
     1321 ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg
     1381 gcaactgcgt gcacttcgtg gccgaggagc aggactaagc agtcaggcag agtcgttgtt
     1441 gggtgtatgg ttgcacaaag ctagacctcg agctggtcgg ttagaaagag atgcttgtgc
     1501 gactggatgt aacaggcaca atccgaggaa aatgcaacag aaacgttatg atgtaacatc
     1561 tgagcacagt caataaaatt gcatgttgta tgccgcacac aaaagtgttg ttaaacatgc
     1621 gatgtggttg tcaaatcatt attcgcacaa ccatggaccg aacctctggc acgctgcata
     1681 cgctgtattc attcacatca tgttttctac ttgtcacatg ctctgcacca agtaactccg
     1741 attagttagg ttcgcacatg tcatcggagt ggaccacttc gaatcatttg gttcgaagac
     1801 aaaccgggaa ttcgacctgc agccaagctt ggcactggcc gtcgttttac aacgtcgtga
     1861 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1921 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1981 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2041 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2101 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2161 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2221 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2281 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2341 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2401 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2461 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2521 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2581 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2641 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2701 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2761 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2821 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2881 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     2941 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3001 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3061 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3121 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3181 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3241 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3301 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3361 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3421 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3481 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3541 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3601 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3661 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3721 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3781 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3841 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3901 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3961 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4021 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4081 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4141 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4201 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     4261 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     4321 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     4381 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     4441 cagctatgac catgattacg aattcccatg g
//
