LOCUS       40924_34371        4151 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pPESiTa, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Berendzen KW, Breuer F, Oberschall A, Schell J, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   2  (bases 1 to 4151)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4151)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..805
                     /label=N-term strep-tag II
                     /note="N-term strep-tag II"
     intron          806..994
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    995..1009
                     /label=C-term strep-tag II
                     /note="C-term strep-tag II"
     misc_feature    1010..1035
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1036..1288
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1320..1338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1368..1384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1392..1408)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1416..1446)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1461..1482)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1770..2358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2532..3389)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3390..3494)
                     /label=AmpR promoter
     rep_origin      3521..3976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4117..4133
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tctaactggt ctcacgtaag tttctgcttc tacctttgat atatatataa
      841 taattatcat taattagtag taatataata ttttaaatat ttttttcaaa ataaaagaat
      901 gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatatttcaa tttataactt
      961 ttctaatata tgaccaaaat ttgttgatgt gcagcctcag ttcgcgaagg tcgacggatc
     1021 cactgcagtg aattctcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     1081 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     1141 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     1201 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     1261 cgcggtgtca tctgtcgagg gggggcccgg taccgagctc gcggccgctc gacgagctcc
     1321 ctatagtgag tcgtattana ggccgacttg gccaaattcg taatcatgtc atagctgttt
     1381 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1441 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1501 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1561 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1621 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1681 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1741 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1801 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1861 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1921 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     1981 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     2041 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     2101 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     2161 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     2221 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     2281 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     2341 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     2401 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     2461 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2521 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2581 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2641 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2701 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2761 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2821 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2881 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2941 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3001 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3061 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3121 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3181 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3241 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3301 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3361 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3421 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3481 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     3541 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3601 gggcccggtc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3661 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3721 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3781 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3841 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     3901 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3961 ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     4021 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     4081 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     4141 tgacttggtc a
//
