LOCUS       62056_18380        6091 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..572
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     CDS             709..2136
                     /codon_start=1
                     /label=tdTomato
                     /note="tandem dimeric (pseudo-monomeric) derivative of
                     DsRed (Shaner et al., 2004)"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     promoter        3836..3940
                     /label=AmpR promoter
     CDS             3941..4798
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4972..5560
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5848..5869
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5884..5914
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5922..5938
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5946..5962
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgaccatgat gataaacaat gtatggtgct aatgttgctt
       61 caacaacaat tctgttgaac tgtgttttca tgtttgccaa caagcacctt tatactcggt
      121 ggcctcccca ccaccaactt ttttgcactg caaaaaaaca cgcttttgca cgcgggccca
      181 tacatagtac aaactctacg tttcgtagac tattttacat aaatagtcta caccgttgta
      241 tacgctccaa atacactacc acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg
      301 gcagtcacgt aggccggcct tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg
      361 accggacgag tgttgtctta tcgtgacagg acgccagctt cctgtgttgc taaccgcagc
      421 cggacgcaac tccttatcgg aacaggacgc gcctccatat cagccgcgcg ttatctcatg
      481 cgcgtgaccg gacacgaggc gcccgtcccg cttatcgcgc ctataaatac agcccgcaac
      541 gatctggtaa acacagttga acagcatctg ttggatcccc gggattggcc aaaggaccca
      601 aaggtatgtt tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggagg
      661 gtaccgagct cagaaaaaat gactgctcca aagaagaagc gtaaggtaat ggtgagcaag
      721 ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac
      781 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      841 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc
      901 cagttcatgt acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag
      961 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt
     1021 ctggtgaccg tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag
     1081 atgcgcggca ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg
     1141 gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag
     1201 gccctgaagc tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc
     1261 aagaagcccg tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc
     1321 cacaacgagg actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg
     1381 ttcctggggc atggcaccgg cagcaccggc agcggcagct ccggcaccgc ctcctccgag
     1441 gacaacaaca tggccgtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
     1501 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1561 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
     1621 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
     1681 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1741 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
     1801 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
     1861 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
     1921 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
     1981 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
     2041 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
     2101 cacctgttcc tgtacggcat ggacgagctg tacaagtagt aaccatacat atttaaattt
     2161 tcaggtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     2221 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     2281 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttgt aagtttaaac
     2341 atgattttac taactaacta atctgattta aattttcaga acttcaaaat tagacacaac
     2401 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
     2461 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
     2521 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
     2581 catggcatgg atgaactata caaatagcat tcgtagaatt ccaactgagc gccggtcgct
     2641 accattacca acttgtctgg tgtcaaaaat aataggggcc gctgtcatca gagtaagttt
     2701 aaactgagtt ctactaacta acgagtaata tttaaatttt cagcatctcg cgcccgtgcc
     2761 tctgacttct aagtccaatt actcttcaac atccctacat gctctttctc cctgtgctcc
     2821 caccccctat ttttgttatt atcaaaaaac ttctcttaat ttctttgttt tttagcttct
     2881 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     2941 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     3001 acaatgttct gtgtacactt cttatgtttt ttacttctga taaatttttt tgaaacatca
     3061 tagaaaaaac cgcacacaaa ataccttatc atatgttacg tttcagttta tgaccgcaat
     3121 ttttatttct tcgcacgtct gggcctctca tgacgtcaaa tcatgctcat cgtgaaaaag
     3181 ttttggagta tttttggaat ttttcaatca agtgaaagtt tatgaaatta attttcctgc
     3241 ttttgctttt tggggtttcc cctattgttt gtcaagattt cgaggacggc gtttttcttg
     3301 ctaaaatcac aagtattgat gagcacgatg caagaaagat cggaagaagg tttgggtttg
     3361 aggctcagtg gaaggtgagt agaagttgat aatttgaaag tggagtagtg tctatggggt
     3421 ttttgcctta aatgacagaa tacattccca atataccaaa cataactgtt tcctactagt
     3481 cggccgtacg ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     3541 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     3601 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     3661 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     3721 agaaaatacc gcatcaggcg gccttaaggg cctcgtgata cgcctatttt tataggttaa
     3781 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3841 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     3901 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     3961 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4021 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4081 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4141 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4201 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4261 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4321 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4381 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4441 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4501 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4561 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4621 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4681 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4741 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4801 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4861 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     4921 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     4981 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5041 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5101 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5161 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5221 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5281 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5341 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     5401 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5461 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5521 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5581 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5641 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5701 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5761 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5821 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     5881 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     5941 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
     6001 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
     6061 gatggatacg ctaacaactt ggaaatgaaa t
//
