LOCUS       40924_34341        6680 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_037_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6680)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6680)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6680)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6680
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(471..1271)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1272..1492)
                     /label=URA3 promoter
     rep_origin      complement(2091..2971)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3320..4111)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4112..4216)
                     /label=AmpR promoter
     regulatory      4372..4938
                     /label=UBI4 promoter
                     /note="UBI4 promoter"
                     /regulatory_class="promoter"
     CDS             4947..5660
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5672..6087
                     /label=UBI4 terminator
                     /note="UBI4 terminator"
                     /regulatory_class="terminator"
     promoter        6094..6314
                     /label=URA3 promoter
     rep_origin      6435..6680
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
       61 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      361 ctttttacgg ttcctggcct tttgctggcc ttttgctcat gatataatta aattgaagct
      421 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
      481 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
      541 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
      601 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
      661 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
      721 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
      781 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
      841 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
      901 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
      961 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     1021 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     1081 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     1141 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     1201 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     1261 gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag
     1321 aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     1381 ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     1441 agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc
     1501 ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga ctatactaga
     1561 tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac
     1621 cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc
     1681 ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc
     1741 tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa
     1801 tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta
     1861 cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata
     1921 gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta
     1981 tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc
     2041 cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat
     2101 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     2161 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     2221 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc taatttttca
     2281 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     2341 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     2401 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     2461 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     2521 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     2581 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     2641 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     2701 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     2761 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     2821 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     2881 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     2941 gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt gataatcaga
     3001 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3361 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3421 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3481 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     3541 catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     3601 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     3661 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     3721 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     3781 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     3841 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     3901 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     3961 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4021 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4081 aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca
     4141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcggacgga tcgcttgcct
     4261 gtaacttaca cgcgcctcgt ggcgcgcccc tgcaggattt aaatggccgg ccgtttaaac
     4321 cttgtacacc taccggtcac cagccaacaa tgtgcggatg gcgttacaac tttttagagt
     4381 cttccttgac caatcatctt attcgcgcag ggcaacccat gataggaaat gtccctttaa
     4441 acgaactgtg ggaaatcctg caaaagtgat gaaagtgacg ttttatgaaa agaaagtgaa
     4501 aaagttcttg tttgacaata ttctattccg aaaagtcttt gtggtcagta ttgtttctag
     4561 aacgttctag aataatcctg gataaaccaa tttcggtacc aaaaaaaaaa atagccgccc
     4621 agtggagcat cacacagccg tacatccggc cacacacggt ggttacccca cttcgtttct
     4681 tttttcaggg gcgatgccac ttatcagttg tttttagaat tcagtgtcat ttgagggcgg
     4741 ttcctccttt tgggggtata tatagagagg ctccgggttt tgccaccttt gaattcgcct
     4801 gcttatcttt cttcttccga aagtgctact tcagaaagag caagaactgt acggataagg
     4861 ataagtatat cttctatcta ctacaatacg acaagaactc tcgaactctc cctcccactt
     4921 tactttaact aatagattgc ggccgcatgt ctaaaggtga agaattattc actggtgttg
     4981 tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct gtctccggtg
     5041 aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt actactggta
     5101 aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt caatgttttg
     5161 ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg ccagaaggtt
     5221 atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc agagctgaag
     5281 tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt gattttaaag
     5341 aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac aatgtttaca
     5401 tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga cacaacattg
     5461 aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt ggtgatggtc
     5521 cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc aaagatccaa
     5581 acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt attacccatg
     5641 gtatggatga attgtacaaa taagcggccg ctcagtcctc gcaatatttt cattatgtca
     5701 atatatatat gtttactctc cttttttctt tttggttttt tttttttttt gataaatact
     5761 ccatagaaca ctaaataaat tgttcaactg tgttattgtc tttattcatg ttggttttca
     5821 agagcttgga tttttgaatc gtcttatact atgacgttca ctattttcgc gaagccgggt
     5881 aataccatta gctattttga tagaaaggga tttttattag ggaatataaa ccacctttat
     5941 agtgtcctat catgtttcaa tctccagtaa acgcacataa gccgaccaat tgagtcaacc
     6001 ttttaactct atttaatttg atacggatag aatattgtga ctaccaaaag ggaaaaggca
     6061 gaaaaaagga aaattaagaa cagttaatag cttttcaatt caattcatca tttttttttt
     6121 attctttttt ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga
     6181 aggaagaacg aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg
     6241 aagaaacatg aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga
     6301 aacgaagata aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc
     6361 ctgtttgaga tttaaatggc gcgccgttta aaccctgcag gggccggccc cgtagaaaag
     6421 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6481 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6541 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6601 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6661 ttaccagtgg ctgctgccag
//
