LOCUS       40924_34331        7022 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_035_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 7022)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 7022)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7022)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7022
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(475..1275)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1276..1496)
                     /label=URA3 promoter
     rep_origin      complement(2095..2975)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3324..4115)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4116..4220)
                     /label=AmpR promoter
     regulatory      4376..5375
                     /label=EFT2 promoter
                     /note="EFT2 promoter"
                     /regulatory_class="promoter"
     CDS             5384..6097
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      6109..6433
                     /label=EFT2 terminator
                     /note="EFT2 terminator"
                     /regulatory_class="terminator"
     promoter        6440..6660
                     /label=URA3 promoter
     rep_origin      6781..7022
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
       61 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
      121 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      181 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      241 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      301 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      361 cggccttttt acggttcctg gccttttgct ggccttttgc tcatgatata attaaattga
      421 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
      481 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
      541 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
      601 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
      661 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
      721 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
      781 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
      841 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
      901 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
      961 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
     1021 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
     1081 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
     1141 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
     1201 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
     1261 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
     1321 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
     1381 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
     1441 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc
     1501 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac
     1561 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
     1621 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
     1681 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
     1741 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
     1801 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
     1861 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     1921 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     1981 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     2041 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     2101 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     2161 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     2221 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt
     2281 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     2341 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     2401 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     2461 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     2521 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     2581 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     2641 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     2701 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     2761 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     2821 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     2881 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     2941 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat
     3001 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aattctttcc
     3061 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagggtct
     3181 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3301 gagtaaactt ggtctgacag tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     3361 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     3421 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     3481 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     3541 caggcatcgt catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     3601 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     3661 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     3721 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     3781 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     3841 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     3901 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     3961 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     4021 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     4081 ggagaacctg cgtgcaatcc atcttgttca atcatactct tcctttttca atattattga
     4141 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     4201 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgcgga cggatcgctt
     4261 gcctgtaact tacacgcgcc tcgtggcgcg cccctgcagg atttaaatgg ccggccgttt
     4321 aaaccttgta cacctaccgg tcaccagcca acaatgtgcg gatggcgtta caacttctca
     4381 aatccattgg tgtttccatt aggccctgag cggttcccca agaaaaaaaa aaacagtaga
     4441 aaaaaatgat gcttcttcct aacgctattg tagtgcaggt tctgccgtgt gccaagttgg
     4501 agcagcgcct gctgagactc ttccgtatgg ttcttgctct tctcaggacc agcggcggca
     4561 gttccggttt ttttcggcgt cttttgtctg gcacacaatg ggcgatggag agggaaaacc
     4621 ggaggatatg ccagctgcgg tgagattatg gttggagtta tgtttgtagc cggcggtgcg
     4681 gcaataggag aggggagggg aaggggaggg gaaaaggtaa tggctgacga agtatagaga
     4741 tggcaatcac aagccaaata ttggagatta catgcaagaa tgctactgtt atacaaaagg
     4801 gtgaaaattg agacccaaat tccttccttt tttttctttt ttttttacca gaagaattac
     4861 gtacaaaagt acctactatt tcaaagcaag aaatgagatg cctattgtgg ttatatacag
     4921 aatagatgat aaatgggttt tccgtgcaaa acgatatgga gaattcaaaa tgggtgcgaa
     4981 atacctggaa cgtaagcgtt ctgagaaata cacagacgca ttaacctgac aaaaacacaa
     5041 ctagtttggg aaagggattt ggtctttcct ctcgggtctc tcgtgtggtt cctttctttc
     5101 tcagatctcc ctgcacactg ggctgttgtc ctccaggtta tggtttgttc tcttcaggta
     5161 ttacaatgca gtaggctttt ggagtgagca aaacgaagag agaaaaaaat tttttcttaa
     5221 aagttttttt tcattttgtg agcttattct tcttttctat atattcttga tatcttagat
     5281 tatacatatt attctcttac atttcacgat tgcccttttg gtgtttagca ttcagactca
     5341 aagaccacaa acacaaacta taacataatt gcaaggcggc cgcatgtcta aaggtgaaga
     5401 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa
     5461 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt
     5521 tatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttcggtta
     5581 tggtgttcaa tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc
     5641 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta
     5701 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa
     5761 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa
     5821 ctctcacaat gtttacatca tggctgacaa acaaaagaat ggtatcaaag ttaacttcaa
     5881 aattagacac aacattgaag atggttctgt tcaattagct gaccattatc aacaaaatac
     5941 tccaattggt gatggtccag tcttgttacc agacaaccat tacttatcca ctcaatctgc
     6001 cttatccaaa gatccaaacg aaaagagaga ccacatggtc ttgttagaat ttgttactgc
     6061 tgctggtatt acccatggta tggatgaatt gtacaaataa gcggccgcga atggttaaac
     6121 aatttttaat tatttaactt tttcagtttt tgtcgtaatg tattgggcac cttttatgtc
     6181 cttttgactt ttttgtagtt tattctcacg tatacttacc atctatagtg ttatttcata
     6241 tttaatcata tttccatatt agatatctgc cttcccctgt ataatagtta ctatgattta
     6301 tcttgctttg cctattcgcg tcatcaactt cttttcttac cgatcgcggt aatgcccttt
     6361 aagagtggca tcaacattgg cgtaaacaaa gtttcaaagg attgatacga acacacattc
     6421 ctagcatgaa agctagcttt tcaattcaat tcatcatttt ttttttattc ttttttttga
     6481 tttcggtttc tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     6541 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     6601 tgcccagtat tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc
     6661 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt ttgagattta
     6721 aatggcgcgc cgtttaaacc ctgcaggggc cggccccgta gaaaagatca aaggatcttc
     6781 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6841 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6901 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6961 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7021 tg
//
