LOCUS       40924_34326        6539 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_034_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6539)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6539)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(470..1270)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1271..1491)
                     /label=URA3 promoter
     rep_origin      complement(2090..2970)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3319..4110)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4111..4215)
                     /label=AmpR promoter
     regulatory      4371..5046
                     /label=HHF2 promotor
                     /note="HHF2 promotor"
                     /regulatory_class="promoter"
     CDS             5055..5768
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5780..5945
                     /label=HHF2 terminator
                     /note="HHF2 terminator"
                     /regulatory_class="terminator"
     promoter        5952..6172
                     /label=URA3 promoter
     rep_origin      6293..6539
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
       61 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      361 tttttacggt tcctggcctt ttgctggcct tttgctcatg atataattaa attgaagctc
      421 taatttgtga gtttagtata catgcattta cttataatac agttttttag ttttgctggc
      481 cgcatcttct caaatatgct tcccagcctg cttttctgta acgttcaccc tctaccttag
      541 catcccttcc ctttgcaaat agtcctcttc caacaataat aatgtcagat cctgtagaga
      601 ccacatcatc cacggttcta tactgttgac ccaatgcgtc tcccttgtca tctaaaccca
      661 caccgggtgt cataatcaac caatcgtaac cttcatctct tccacccatg tctctttgag
      721 caataaagcc gataacaaaa tctttgtcgc tcttcgcaat gtcaacagta cccttagtat
      781 attctccagt agatagggag cccttgcatg acaattctgc taacatcaaa aggcctctag
      841 gttcctttgt tacttcttct gccgcctgct tcaaaccgct aacaatacct gggcccacca
      901 caccgtgtgc attcgtaatg tctgcccatt ctgctattct gtatacaccc gcagagtact
      961 gcaatttgac tgtattacca atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt
     1021 acttggcgga taatgccttt agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga
     1081 tatccacatg tgtttttagt aaacaaattt tgggacctaa tgcttcaact aactccagta
     1141 attccttggt ggtacgaaca tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga
     1201 tattaaatag cttggcagca acaggactag gatgagtagc agcacgttcc ttatatgtag
     1261 ctttcgacat gatttatctt cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga
     1321 atactgggca atttcatgtt tcttcaacac tacatatgcg tatatatacc aatctaagtc
     1381 tgtgctcctt ccttcgttct tccttctgtt cggagattac cgaatcaaaa aaatttcaaa
     1441 gaaaccgaaa tcaaaaaaaa gaataaaaaa aaaatgatga attgaattga aaagctagct
     1501 tatcgatgat aagctgtcaa agatgagaat taattccacg gactatagac tatactagat
     1561 actccgtcta ctgtacgata cacttccgct caggtccttg tcctttaacg aggccttacc
     1621 actcttttgt tactctattg atccagctca gcaaaggcag tgtgatctaa gattctatct
     1681 tcgcgatgta gtaaaactag ctagaccgag aaagagacta gaaatgcaaa aggcacttct
     1741 acaatggctg ccatcattat tatccgatgt gacgctgcag cttctcaatg atattcgaat
     1801 acgctttgag gagatacagc ctaatatccg acaaactgtt ttacagattt acgatcgtac
     1861 ttgttaccca tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag
     1921 tatatttgaa cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat
     1981 ttcggttcct ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc
     2041 ggttcatttt ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc
     2101 tgtgcttcat tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     2161 tctgagctgc atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag
     2221 aatctgtgct tcatttttgt aaaacaaaaa tgcaacgcga cgagagcgct aatttttcaa
     2281 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     2341 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     2401 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     2461 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     2521 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     2581 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     2641 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     2701 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     2761 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     2821 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     2881 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     2941 agcaatgttt gtggaagcgg tattcgcaat gggaagctcc accccggttg ataatcagaa
     3001 aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaattc tttcctgcgt
     3061 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag ggtctgacgc
     3181 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3241 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3301 aacttggtct gacagtcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     3361 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     3421 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     3481 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     3541 atcgtcatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa
     3601 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     3661 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     3721 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     3781 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     3841 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     3901 cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt
     3961 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     4021 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     4081 acctgcgtgc aatccatctt gttcaatcat actcttcctt tttcaatatt attgaagcat
     4141 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     4201 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gcggacggat cgcttgcctg
     4261 taacttacac gcgcctcgtg gcgcgcccct gcaggattta aatggccggc cgtttaaacc
     4321 ttgtacacct accggtcacc agccaacaat gtgcggatgg cgttacaact tgtggagtgt
     4381 ttgcttggat cctttagtaa aaggggaaga acagttggaa gggccaaagt ggaagtcaca
     4441 aaacagtggt cctatataaa agaacaagaa aaagattatt tatatacaac tgcggtcaca
     4501 agaagcaacg cgagagagca caacacgctg ttatcacgca aactatgttt tgacaccgag
     4561 ccatagccgt gattgtgcgt cacattgggc gataatgaac gctaaatgac caactcccat
     4621 ccgtaggagc cccttagggc gtgccaatag tttcacgcgc ttaatgcgaa gtgctcggaa
     4681 cggacaactg tggtcgtttg gcaccgggaa agtggtacta gaccgagagt ttcgcatttg
     4741 tatggcagga cgttctggga gcttcgcgtc tcaagctttt tcgggcgcga aatgcagacc
     4801 agaccagaac aaaacaactg acaagaaggc gtttaattta atatgttgtt cactcgcgcc
     4861 tgggctgttg ttattcggct agatacatac gtgtttgtgc gtatgtagtt atatcatata
     4921 taagtatatt aggatgaggc ggtgaaagag attttttttt tttcgcttaa tttattcttt
     4981 tctctatctt ttttcctaca tcttgttcaa aagagtagca aaaacaacaa tcaatacaat
     5041 aaaatagcgg ccgcatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
     5101 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
     5161 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
     5221 catggccaac cttagtcact actttcggtt atggtgttca atgttttgct agatacccag
     5281 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
     5341 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
     5401 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     5461 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
     5521 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
     5581 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     5641 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     5701 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     5761 tgtacaaata agcggccgca caatcggtgg ttaaacaatc ggtgtttgaa attattttca
     5821 tgcctttcaa aaaataaaat aacgatcatt ctattgggaa tataatagaa ccttatgtat
     5881 gcatttttcg gttttttatt atttcatgta ctatatatat taaaaataag tctcaataaa
     5941 agaaatagct tttcaattca attcatcatt ttttttttat tctttttttt gatttcggtt
     6001 tctttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa ggaaggagca
     6061 cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa attgcccagt
     6121 attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa
     6181 agctacatat aaggaacgtg ctgctactca tcctagtcct gtttgagatt taaatggcgc
     6241 gccgtttaaa ccctgcaggg gccggccccg tagaaaagat caaaggatct tcttgagatc
     6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagt
//
