LOCUS       40924_34321        7082 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_033_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 7082)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7082)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(476..1276)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1277..1497)
                     /label=URA3 promoter
     rep_origin      complement(2096..2976)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3325..4116)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4117..4221)
                     /label=AmpR promoter
     regulatory      4377..5376
                     /label=CCW12 promoter
                     /note="CCW12 promoter"
                     /regulatory_class="promoter"
     CDS             5385..6098
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      6110..6494
                     /label=CCW12 terminator
                     /note="CCW12 terminator"
                     /regulatory_class="terminator"
     promoter        6501..6721
                     /label=URA3 promoter
     rep_origin      6842..7082
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
       61 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
      361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcatgatat aattaaattg
      421 aagctctaat ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt
      481 gctggccgca tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta
      541 ccttagcatc ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg
      601 tagagaccac atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta
      661 aacccacacc gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc
      721 tttgagcaat aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct
      781 tagtatattc tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc
      841 ctctaggttc ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc
      901 ccaccacacc gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag
      961 agtactgcaa tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa
     1021 aattgtactt ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag
     1081 tcaagatatc cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact
     1141 ccagtaattc cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt
     1201 gcatgatatt aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat
     1261 atgtagcttt cgacatgatt tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg
     1321 ttaagaatac tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc
     1381 taagtctgtg ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat
     1441 ttcaaagaaa ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag
     1501 ctagcttatc gatgataagc tgtcaaagat gagaattaat tccacggact atagactata
     1561 ctagatactc cgtctactgt acgatacact tccgctcagg tccttgtcct ttaacgaggc
     1621 cttaccactc ttttgttact ctattgatcc agctcagcaa aggcagtgtg atctaagatt
     1681 ctatcttcgc gatgtagtaa aactagctag accgagaaag agactagaaa tgcaaaaggc
     1741 acttctacaa tggctgccat cattattatc cgatgtgacg ctgcagcttc tcaatgatat
     1801 tcgaatacgc tttgaggaga tacagcctaa tatccgacaa actgttttac agatttacga
     1861 tcgtacttgt tacccatcat tgaattttga acatccgaac ctgggagttt tccctgaaac
     1921 agatagtata tttgaacctg tataataata tatagtctag cgctttacgg aagacaatgt
     1981 atgtatttcg gttcctggag aaactattgc atctattgca taggtaatct tgcacgtcgc
     2041 atccccggtt cattttctgc gtttccatct tgcacttcaa tagcatatct ttgttaacga
     2101 agcatctgtg cttcattttg tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac
     2161 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgaaagcgc tattttacca
     2221 acgaagaatc tgtgcttcat ttttgtaaaa caaaaatgca acgcgacgag agcgctaatt
     2281 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta
     2341 ttttaccaac aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc
     2401 tatttttcta acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca
     2461 gtctcttgat aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg
     2521 tctattttct cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc
     2581 gaagctgcgg gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt
     2641 ggattgcgca tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa
     2701 aattatgaac ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt
     2761 ttcgtattgt tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga
     2821 gtaatactag agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag
     2881 cgaaaggtgg atgggtaggt tatataggga tatagcacag agatatatag caaagagata
     2941 cttttgagca atgtttgtgg aagcggtatt cgcaatggga agctccaccc cggttgataa
     3001 tcagaaaagc cccaaaaaca ggaagattgt ataagcaaat atttaaattg taattctttc
     3061 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3121 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagggtc
     3181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3301 tgagtaaact tggtctgaca gtcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     3361 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     3421 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     3481 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     3541 gcaggcatcg tcatgggtca cgacgagatc ctcgccgtcg ggcatgctcg ccttgagcct
     3601 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     3661 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     3721 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     3781 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     3841 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     3901 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     3961 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     4021 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     4081 cggagaacct gcgtgcaatc catcttgttc aatcatactc ttcctttttc aatattattg
     4141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcgg acggatcgct
     4261 tgcctgtaac ttacacgcgc ctcgtggcgc gcccctgcag gatttaaatg gccggccgtt
     4321 taaaccttgt acacctaccg gtcaccagcc aacaatgtgc ggatggcgtt acaactaacc
     4381 agggcaaagc aaaataaaag aaacttaata cgttatgccg taatgaaggg ctaccaaaaa
     4441 cgataatctc aactgtaaac aggtacaatg cggacccttt tgccacaaaa catacatcat
     4501 tcattgccgg aaaaagaaag aagtgaagac agcagtgcag ccagccatgt tgcgccaatc
     4561 taattataga tgctggtgcc ctgaggatgt atctggagcc agccatggca tcatgcgcta
     4621 ccgccggatg taaaatccga cacgcaaaag aaaaccttcg aggttgcgca cttcgcccac
     4681 ccatgaacca cacggttagt ccaaaagggg cagttcagat tccagatgcg ggaattagct
     4741 tgctgccacc ctcacctcac taacgctgcg gtgtgcggat acttcatgct atttatagac
     4801 gcgcgtgtcg gaatcagcac gcgcaagaac caaatgggaa aatcggaatg ggtccagaac
     4861 tgctttgagt gctggctatt ggcgtctgat ttccgttttg ggaatccttt gccgcgcgcc
     4921 cctctcaaaa ctccgcacaa gtcccagaaa gcgggaaaga aataaaacgc caccaaaaaa
     4981 aaaaaaataa aagccaatcc tcgaagcgtg ggtggtaggc cctggattat cccgtacaag
     5041 tatttctcag gagtaaaaaa accgtttgtt ttggaattcc ccatttcgcg gccacctacg
     5101 ccgctatctt tgcaacaact atctgcgata actcagcaaa ttttgcatat tcgtgttgca
     5161 gtattgcgat aatgggagtc ttacttccaa cataacggca gaaagaaatg tgagaaaatt
     5221 ttgcatcctt tgcctccgtt caagtatata aagtcggcat gcttgataat ctttctttcc
     5281 atcctacatt gttctaatta ttcttattct cctttattct ttcctaacat accaagaaat
     5341 taatcttctg tcattcgctt aaacactata tcaatagcgg ccgcatgtct aaaggtgaag
     5401 aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt aatggtcaca
     5461 aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg accttaaaat
     5521 ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact actttcggtt
     5581 atggtgttca atgttttgct agatacccag atcatatgaa acaacatgac tttttcaagt
     5641 ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat gacggtaact
     5701 acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga atcgaattaa
     5761 aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa tacaactata
     5821 actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa gttaacttca
     5881 aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat caacaaaata
     5941 ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc actcaatctg
     6001 ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg
     6061 ctgctggtat tacccatggt atggatgaat tgtacaaata agcggccgca cttagtttat
     6121 tattatttat acattctaaa ttttttataa acttttttgg catttaacaa atatataaca
     6181 attgggattt agtagaaatt cttttaattt cttctttttt tttttaatct atatttattc
     6241 tttttcttta attttgttcg aaacgcatat aatgttttta ttgattttgt ttcaggttcc
     6301 tgaacctcgc ggagttccta ttttactgtt gttttttttc aaaacacagg ggtaccttga
     6361 agtatacgca gtgcctctta gccaaggata atataaacac actagaacaa aaaaaaaata
     6421 ctccttgtac agaatcgttt tctattttga atttttttta tataaacatg gttttattgt
     6481 ctattacata gcattagctt ttcaattcaa ttcatcattt tttttttatt cttttttttg
     6541 atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag
     6601 gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa
     6661 ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat
     6721 catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg tttgagattt
     6781 aaatggcgcg ccgtttaaac cctgcagggg ccggccccgt agaaaagatc aaaggatctt
     6841 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6901 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6961 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     7021 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     7081 ct
//
