LOCUS       40924_34311        6926 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_031_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6926)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6926)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6926
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(481..1281)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1282..1502)
                     /label=URA3 promoter
     rep_origin      complement(2101..2981)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3330..4121)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4122..4226)
                     /label=AmpR promoter
     regulatory      4382..5381
                     /label=RPL3 promoter
                     /note="RPL3 promoter"
                     /regulatory_class="promoter"
     CDS             5390..6103
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      6115..6343
                     /label=RPL3 terminator
                     /note="RPL3 terminator"
                     /regulatory_class="terminator"
     promoter        6350..6570
                     /label=URA3 promoter
     rep_origin      6691..6926
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
       61 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcat gatataatta
      421 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
      481 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
      541 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
      601 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
      661 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
      721 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
      781 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
      841 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
      901 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
      961 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     1021 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     1081 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     1141 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     1201 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     1261 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
     1321 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     1381 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     1441 aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg
     1501 aaaagctagc ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga
     1561 ctatactaga tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac
     1621 gaggccttac cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta
     1681 agattctatc ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa
     1741 aaggcacttc tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat
     1801 gatattcgaa tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt
     1861 tacgatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     1921 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     1981 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     2041 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     2101 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     2161 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     2221 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc
     2281 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     2341 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     2401 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     2461 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     2521 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     2581 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     2641 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     2701 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     2761 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     2821 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     2881 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     2941 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt
     3001 gataatcaga aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt
     3061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3301 atatatgagt aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg
     3361 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3421 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     3481 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     3541 ggcaagcagg catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     3601 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     3661 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     3721 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     3781 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     3841 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     3901 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     3961 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4021 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4081 gcggccggag aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat
     4141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcggacgga
     4261 tcgcttgcct gtaacttaca cgcgcctcgt ggcgcgcccc tgcaggattt aaatggccgg
     4321 ccgtttaaac cttgtacacc taccggtcac cagccaacaa tgtgcggatg gcgttacaac
     4381 tcctttcctg cgggatagct tttgcttcag gttcttctga ggttctgaaa atcaatatat
     4441 ttttccgaca atttctcact atgcctctat cccccttaaa aagaaacact ttctaccctc
     4501 ttttctaaac tctgcacact agaccgagac agcaaatcgg agcccgagag aaaatccgga
     4561 ggacggaaaa actagaagta atcttatgcg gggagcatgc accagaacgc ccagaaaacg
     4621 tgaatgacac tacatacttt gatacggatg ttggtatttt tccggctttt tctattttat
     4681 cgatgccgtg tctgctgcca ttacgtacag gtaggcaaag gcaaacaaga atagcacttt
     4741 ttcacttgct gctttttttt gtgaatgaag tttatcgtat attctgttag tgccgctcgt
     4801 atcagccact tacgttatca ttctaaagta agtatacgaa atgtgcaaac cttagtagac
     4861 gttcaagaat tgctcgataa ttgcgaacaa accagctgct aagccgggaa gttaagtcct
     4921 caacacagaa atgatgcaaa caatttatta aattcagtgg taatgcaaca gcaagaggaa
     4981 aggtggaggg attaacgcat ttcagacagc tttatagggg gaaagaaagc actcgcaaac
     5041 ttgctgcctg ttcgcagtca ttggttgcaa aaactaaact ctactcacgc acactggaat
     5101 gaatggcaat attctttttt aggttaaccg gccggacagt aatatagtaa tcgttttgta
     5161 cgtttttcaa gaagcgacgc acaactgttt tccatttttt tttttttttt ttcagtgatc
     5221 atcgtccatg aaaaaaattt ttcatttgtc tctttcgtgc ttcctggata tataaaatac
     5281 gatttattta gttgtctttg tcaatcctca tctttcttta ctcattattt catttcggtt
     5341 ttgtcatctc tagaacaaca cagttactac aacaatcaat cgcggccgca tgtctaaagg
     5401 tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg
     5461 tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt
     5521 aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt
     5581 cggttatggt gttcaatgtt ttgctagata cccagatcat atgaaacaac atgacttttt
     5641 caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg
     5701 taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga
     5761 attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa
     5821 ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa
     5881 cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca
     5941 aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatccactca
     6001 atctgcctta tccaaagatc caaacgaaaa gagagaccac atggtcttgt tagaatttgt
     6061 tactgctgct ggtattaccc atggtatgga tgaattgtac aaataagcgg ccgcgaagtt
     6121 ttgttagaaa ataaatcatt ttttaattga gcattcttat tcctatttta tttaaatagt
     6181 tttatgtatt gttagctaca tacaacagtt taaatcaaat tttctttttc ccaagtccaa
     6241 aatggaggtt tattttgatg acccgcatgc gattatgttt tgaaagtata agactacata
     6301 catgtacata tatttaaaca tgtaaacccg tccattatat tgctagcttt tcaattcaat
     6361 tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg
     6421 gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
     6481 cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
     6541 acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct
     6601 gctactcatc ctagtcctgt ttgagattta aatggcgcgc cgtttaaacc ctgcaggggc
     6661 cggccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6901 cgctctgcta atcctgttac cagtgg
//
