LOCUS       40924_34306        7197 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_030_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 7197)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7197)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(479..1279)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1280..1500)
                     /label=URA3 promoter
     rep_origin      complement(2099..2979)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3328..4119)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4120..4224)
                     /label=AmpR promoter
     regulatory      4380..5379
                     /label=CWP2 promotor
                     /note="CWP2 promotor"
                     /regulatory_class="promoter"
     CDS             5388..6101
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      6113..6612
                     /label=CWP2 terminator
                     /note="CWP2 terminator"
                     /regulatory_class="terminator"
     promoter        6619..6839
                     /label=URA3 promoter
     rep_origin      6960..7197
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
       61 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcatga tataattaaa
      421 ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt
      481 tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct
      541 ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc
      601 ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat
      661 ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt
      721 ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac
      781 ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa
      841 ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg
      901 ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg
      961 cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta
     1021 aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat
     1081 cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta
     1141 actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt
     1201 cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct
     1261 tatatgtagc tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt
     1321 gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca
     1381 atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa
     1441 aatttcaaag aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa
     1501 aagctagctt atcgatgata agctgtcaaa gatgagaatt aattccacgg actatagact
     1561 atactagata ctccgtctac tgtacgatac acttccgctc aggtccttgt cctttaacga
     1621 ggccttacca ctcttttgtt actctattga tccagctcag caaaggcagt gtgatctaag
     1681 attctatctt cgcgatgtag taaaactagc tagaccgaga aagagactag aaatgcaaaa
     1741 ggcacttcta caatggctgc catcattatt atccgatgtg acgctgcagc ttctcaatga
     1801 tattcgaata cgctttgagg agatacagcc taatatccga caaactgttt tacagattta
     1861 cgatcgtact tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga
     1921 aacagatagt atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa
     1981 tgtatgtatt tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt
     2041 cgcatccccg gttcattttc tgcgtttcca tcttgcactt caatagcata tctttgttaa
     2101 cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca
     2161 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta
     2221 ccaacgaaga atctgtgctt catttttgta aaacaaaaat gcaacgcgac gagagcgcta
     2281 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg
     2341 ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag
     2401 cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat
     2461 gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg
     2521 gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact
     2581 agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga
     2641 tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca
     2701 gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac
     2761 attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa
     2821 agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag
     2881 gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag
     2941 atacttttga gcaatgtttg tggaagcggt attcgcaatg ggaagctcca ccccggttga
     3001 taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaattct
     3061 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagg
     3181 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3241 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3301 atatgagtaa acttggtctg acagtcagaa gaactcgtca agaaggcgat agaaggcgat
     3361 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     3421 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     3481 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     3541 caagcaggca tcgtcatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag
     3601 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     3661 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     3721 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     3781 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     3841 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     3901 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga
     3961 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     4021 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     4081 ggccggagaa cctgcgtgca atccatcttg ttcaatcata ctcttccttt ttcaatatta
     4141 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4201 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cggacggatc
     4261 gcttgcctgt aacttacacg cgcctcgtgg cgcgcccctg caggatttaa atggccggcc
     4321 gtttaaacct tgtacaccta ccggtcacca gccaacaatg tgcggatggc gttacaactc
     4381 taatagacaa ggtgctatga gtgaattgct agcctcccct ttttattttg tgcggtcacc
     4441 gcaagggaca aagcttttct tagaaaaccg tctgagaagc ataacgtacg ccatccccta
     4501 gacatattaa taatgctaca gatactatgc tgctcgtctt tttttgacga cccttttatt
     4561 gcaatgtgca actaatggca aacaaccaca tagtatcaca gtattacatt gcctccaccg
     4621 atgcggatgt tagggcgcca agtctgtcat gaagcatgtt cctgtcataa tcttgtatgc
     4681 aaaataccgc gttctgcgcc actgatatgc taggcagcag caacctatgc agaagattgc
     4741 ttttcccacg cctgttttac gtctccaggg cacttgaaac aatgcagcga tcgccgccac
     4801 aacacgccaa agagaagcga aagtgggcct gggcggcctc agtttcggca gaggtaaaca
     4861 acacgaactg aactgcctta gctccgaagg gcaattccac aggcactccg cggggcccgg
     4921 ccaaggccca aaaggcgtgg aatatgcgcg ttttggggcc ataacaccca gtaccacggc
     4981 cggaacgggc catataataa gtttttcact ctcaagaatg gtaaacgtaa ataggaacat
     5041 cccactaccc tagaaattgc ggaaatttcg cgcttatcat tagaaaatct ggaaccgtcc
     5101 tttttcctct ttcttgcatt tccctttccg tattattgcc attctttaac tgcatttggg
     5161 gaaccgtaga ccaaaagcca aacagagaaa tgtaacgttc taaaaaaaaa acaacgaaaa
     5221 aattgaaaaa taagatacaa taatcgtata taaatcaggc ttcttgttca tcattttcaa
     5281 ttctcttctt gccatccctt ttcctatctt tgttcttttc ttctcataat caagaataaa
     5341 taacttcatc acattcgcta cacactaaca agaaaaaaag cggccgcatg tctaaaggtg
     5401 aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc
     5461 acaaattttc tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa
     5521 aatttatttg tactactggt aaattgccag ttccatggcc aaccttagtc actactttcg
     5581 gttatggtgt tcaatgtttt gctagatacc cagatcatat gaaacaacat gactttttca
     5641 agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta
     5701 actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat
     5761 taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact
     5821 ataactctca caatgtttac atcatggctg acaaacaaaa gaatggtatc aaagttaact
     5881 tcaaaattag acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa
     5941 atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat
     6001 ctgccttatc caaagatcca aacgaaaaga gagaccacat ggtcttgtta gaatttgtta
     6061 ctgctgctgg tattacccat ggtatggatg aattgtacaa ataagcggcc gcgaaatctc
     6121 tgatttttta taatatctat atggcttttt tcaaaatttt cggttttact aggtaagtgt
     6181 ttgattcttt tcttttcgtt aatatatttt tacacataat tttaaataat ttttgttatt
     6241 ttgaataggt agataccata aaaataaaac actttttact ttaacgatgt tcctgtgatt
     6301 tctcttcgaa tgagtcaaaa atttgaactc gaaatgaatg tattagagac tgggtttctt
     6361 cgagtttgct gagatcttgg ctatgcacaa tgtttcctgc gcccctaagg tgaaaaaaac
     6421 atgcatgctt gtttcaacag accagtgtgc ataaagaatt tcgcccttag ctgcgattaa
     6481 agagtcaagg cgtgaaaaac ataggcgaaa gtgctgaccg ttcttccctt tatcttttta
     6541 ccatcttcgt tatctgttac ctgttcaaaa gaaagtggga aagatattgg tgtgctacgg
     6601 tgggatcttt tttagctttt caattcaatt catcattttt tttttattct tttttttgat
     6661 ttcggtttct ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga
     6721 aggagcacag acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt
     6781 gcccagtatt cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca
     6841 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt tgagatttaa
     6901 atggcgcgcc gtttaaaccc tgcaggggcc ggccccgtag aaaagatcaa aggatcttct
     6961 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7021 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7081 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7141 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggct
//
