LOCUS       40924_34291        6384 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_027_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6384)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6384)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(472..1272)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1273..1493)
                     /label=URA3 promoter
     rep_origin      complement(2092..2972)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3321..4112)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4113..4217)
                     /label=AmpR promoter
     regulatory      4373..4872
                     /label=UBC6 promoter
                     /note="UBC6 promoter"
                     /regulatory_class="promoter"
     CDS             4881..5594
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5606..5792
                     /label=UBC6 terminator
                     /note="UBC6 terminator"
                     /regulatory_class="terminator"
     promoter        5799..6019
                     /label=URA3 promoter
     rep_origin      6140..6384
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
       61 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      361 cctttttacg gttcctggcc ttttgctggc cttttgctca tgatataatt aaattgaagc
      421 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
      481 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
      541 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
      601 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
      661 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
      721 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
      781 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
      841 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
      901 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
      961 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     1021 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     1081 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     1141 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     1201 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     1261 agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa
     1321 gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag
     1381 tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca
     1441 aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctag
     1501 cttatcgatg ataagctgtc aaagatgaga attaattcca cggactatag actatactag
     1561 atactccgtc tactgtacga tacacttccg ctcaggtcct tgtcctttaa cgaggcctta
     1621 ccactctttt gttactctat tgatccagct cagcaaaggc agtgtgatct aagattctat
     1681 cttcgcgatg tagtaaaact agctagaccg agaaagagac tagaaatgca aaaggcactt
     1741 ctacaatggc tgccatcatt attatccgat gtgacgctgc agcttctcaa tgatattcga
     1801 atacgctttg aggagataca gcctaatatc cgacaaactg ttttacagat ttacgatcgt
     1861 acttgttacc catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat
     1921 agtatatttg aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt
     1981 atttcggttc ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc
     2041 ccggttcatt ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca
     2101 tctgtgcttc attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag
     2161 aatctgagct gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga
     2221 agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc gacgagagcg ctaatttttc
     2281 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     2341 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     2401 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     2461 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     2521 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     2581 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     2641 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     2701 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     2761 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     2821 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     2881 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     2941 tgagcaatgt ttgtggaagc ggtattcgca atgggaagct ccaccccggt tgataatcag
     3001 aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaat tctttcctgc
     3061 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3121 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agggtctgac
     3181 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3241 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3301 taaacttggt ctgacagtca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     3361 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     3421 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     3481 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     3541 gcatcgtcat gggtcacgac gagatcctcg ccgtcgggca tgctcgcctt gagcctggcg
     3601 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     3661 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     3721 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     3781 tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     3841 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     3901 gccagccacg atagccgcgc tgcctcgtct tgcagttcat tcagggcacc ggacaggtcg
     3961 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     4021 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     4081 gaacctgcgt gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc
     4141 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4201 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgcggacgg atcgcttgcc
     4261 tgtaacttac acgcgcctcg tggcgcgccc ctgcaggatt taaatggccg gccgtttaaa
     4321 ccttgtacac ctaccggtca ccagccaaca atgtgcggat ggcgttacaa ctattcaacg
     4381 aaacttacga caagatagtc tttggatgat gaggaatatc ctcttcttag caatccctcg
     4441 atgcctcctc atatcatcta ttgttcaaaa ttttttcatt gttgaaaaat agtcacgtgc
     4501 ttaaccattt tgctgcgcga tttggtagga ggataaatac acgggcatac cgtatctgtt
     4561 caatagcaac aatgtcggag agagctaaac tagccaactc gccatcctat tccactggtc
     4621 gcttgagtcg atatccttcc acagcaatat tgcactttac aattaggata gcctccccaa
     4681 taaaataagg aaattttggt tatttgtgtt cagaaaaagc cgaaatcgat tgcagagaac
     4741 agaaaggaac tttccgatta tcacacttta ggagccgtga taaagaagac taccatcgca
     4801 tatcgcagaa aagactttaa atattaacta aaaccgcatt cgcaaattgc aaacaaagta
     4861 cgtacaatag tagcggccgc atgtctaaag gtgaagaatt attcactggt gttgtcccaa
     4921 ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg
     4981 aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc
     5041 cagttccatg gccaacctta gtcactactt tcggttatgg tgttcaatgt tttgctagat
     5101 acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc
     5161 aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt
     5221 ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg
     5281 gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcatgg
     5341 ctgacaaaca aaagaatggt atcaaagtta acttcaaaat tagacacaac attgaagatg
     5401 gttctgttca attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct
     5461 tgttaccaga caaccattac ttatccactc aatctgcctt atccaaagat ccaaacgaaa
     5521 agagagacca catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg
     5581 atgaattgta caaataagcg gccgcagcca ttaaatgaac taaactttag ataaattttg
     5641 acaacacaga atagaaatca atagtattat atagacaaaa cggatctttt tattatgaac
     5701 attgtgaatt attaattata tttttgagta tgtattgacc aagaaacctc aataaaaatg
     5761 aggtgaaacc tagggagaca gaaactccca tttagctttt caattcaatt catcattttt
     5821 tttttattct tttttttgat ttcggtttct ttgaaatttt tttgattcgg taatctccga
     5881 acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac gcatatgtag
     5941 tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa caaaaacctg
     6001 caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg ctactcatcc
     6061 tagtcctgtt tgagatttaa atggcgcgcc gtttaaaccc tgcaggggcc ggccccgtag
     6121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6361 tcctgttacc agtggctgct gcca
//
