LOCUS       40924_34281        6315 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_025_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6315)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(473..1273)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1274..1494)
                     /label=URA3 promoter
     rep_origin      complement(2093..2973)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3322..4113)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4114..4218)
                     /label=AmpR promoter
     regulatory      4374..4873
                     /label=SGA1 promoter
                     /note="SGA1 promoter"
                     /regulatory_class="promoter"
     CDS             4882..5595
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5607..5724
                     /label=SGA1 terminator
                     /note="SGA1 terminator"
                     /regulatory_class="terminator"
     promoter        5731..5951
                     /label=URA3 promoter
     rep_origin      6072..6315
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
       61 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      121 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      181 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      241 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      301 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      361 gcctttttac ggttcctggc cttttgctgg ccttttgctc atgatataat taaattgaag
      421 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
      481 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
      541 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
      601 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
      661 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
      721 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
      781 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
      841 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
      901 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
      961 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     1021 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     1081 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     1141 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     1201 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     1261 tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta
     1321 agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa
     1381 gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc
     1441 aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta
     1501 gcttatcgat gataagctgt caaagatgag aattaattcc acggactata gactatacta
     1561 gatactccgt ctactgtacg atacacttcc gctcaggtcc ttgtccttta acgaggcctt
     1621 accactcttt tgttactcta ttgatccagc tcagcaaagg cagtgtgatc taagattcta
     1681 tcttcgcgat gtagtaaaac tagctagacc gagaaagaga ctagaaatgc aaaaggcact
     1741 tctacaatgg ctgccatcat tattatccga tgtgacgctg cagcttctca atgatattcg
     1801 aatacgcttt gaggagatac agcctaatat ccgacaaact gttttacaga tttacgatcg
     1861 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     1921 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     1981 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     2041 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     2101 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     2161 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     2221 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgacgagagc gctaattttt
     2281 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgag agcgctattt
     2341 taccaacaaa gaatctatac ttcttttttg ttctacaaaa atgcatcccg agagcgctat
     2401 ttttctaaca aagcatctta gattactttt tttctccttt gtgcgctcta taatgcagtc
     2461 tcttgataac tttttgcact gtaggtccgt taaggttaga agaaggctac tttggtgtct
     2521 attttctctt ccataaaaaa agcctgactc cacttcccgc gtttactgat tactagcgaa
     2581 gctgcgggtg cattttttca agataaaggc atccccgatt atattctata ccgatgtgga
     2641 ttgcgcatac tttgtgaaca gaaagtgata gcgttgatga ttcttcattg gtcagaaaat
     2701 tatgaacggt ttcttctatt ttgtctctat atactacgta taggaaatgt ttacattttc
     2761 gtattgtttt cgattcactc tatgaatagt tcttactaca atttttttgt ctaaagagta
     2821 atactagaga taaacataaa aaatgtagag gtcgagttta gatgcaagtt caaggagcga
     2881 aaggtggatg ggtaggttat atagggatat agcacagaga tatatagcaa agagatactt
     2941 ttgagcaatg tttgtggaag cggtattcgc aatgggaagc tccaccccgg ttgataatca
     3001 gaaaagcccc aaaaacagga agattgtata agcaaatatt taaattgtaa ttctttcctg
     3061 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3121 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagggtctga
     3181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3301 gtaaacttgg tctgacagtc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     3361 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     3421 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     3481 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     3541 ggcatcgtca tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc
     3601 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     3661 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     3721 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     3781 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     3841 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     3901 ggccagccac gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc
     3961 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4021 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     4081 agaacctgcg tgcaatccat cttgttcaat catactcttc ctttttcaat attattgaag
     4141 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4201 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgcggacg gatcgcttgc
     4261 ctgtaactta cacgcgcctc gtggcgcgcc cctgcaggat ttaaatggcc ggccgtttaa
     4321 accttgtaca cctaccggtc accagccaac aatgtgcgga tggcgttaca acttatacag
     4381 cttgtgattt cgcgcgccga atgggctgaa aagcacagta tagtggagaa gaagagagag
     4441 gagaagggga gaggggcaat agcaatggat gtggtagaat acagggaagg ccaagctgtt
     4501 gttcaatgga tgcggttcga ggcccagcgc aaggggggcg cttcgaagca tagaacatta
     4561 tccgcggaaa cgggtattag gggtgagggt gaataaggaa agtcagggaa atcgggccag
     4621 agtaacaccc attcatagca ctcgtacaag gtgcttttaa cttgcctgca tgtgtggagt
     4681 cacaaattag gggactcagg cacagaagca agggtccttt tttggttccc tgtttcctcc
     4741 tgcgcatttc gtattttttc tcatctcttg gctctggatc cgttatctgt tctgttacac
     4801 aagaaatcgt acatttacaa tatagtgata atcgtggact agagcaagat ttcaaataag
     4861 taacagcagc aaagcggccg catgtctaaa ggtgaagaat tattcactgg tgttgtccca
     4921 attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc cggtgaaggt
     4981 gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac tggtaaattg
     5041 ccagttccat ggccaacctt agtcactact ttcggttatg gtgttcaatg ttttgctaga
     5101 tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga aggttatgtt
     5161 caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc tgaagtcaag
     5221 tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt taaagaagat
     5281 ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt ttacatcatg
     5341 gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa cattgaagat
     5401 ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga tggtccagtc
     5461 ttgttaccag acaaccatta cttatccact caatctgcct tatccaaaga tccaaacgaa
     5521 aagagagacc acatggtctt gttagaattt gttactgctg ctggtattac ccatggtatg
     5581 gatgaattgt acaaataagc ggccgcacaa aaaaaaaaat aaaaggaaag cgagaagtat
     5641 acacaagtgt atttcctaga tatttacatc aaatatatat atatacttat ttacaaaact
     5701 ctgatattat aaattgatta gatatagctt ttcaattcaa ttcatcattt tttttttatt
     5761 cttttttttg atttcggttt ctttgaaatt tttttgattc ggtaatctcc gaacagaagg
     5821 aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag
     5881 aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac
     5941 gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg
     6001 tttgagattt aaatggcgcg ccgtttaaac cctgcagggg ccggccccgt agaaaagatc
     6061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6181 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6301 ccagtggctg ctgcc
//
