LOCUS       40924_34246        7273 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_018_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 7273)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7273)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(471..1271)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1272..1492)
                     /label=URA3 promoter
     rep_origin      complement(2091..2971)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3320..4111)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4112..4216)
                     /label=AmpR promoter
     regulatory      4372..4948
                     /label=TDH1 promoter
                     /note="TDH1 promoter"
                     /regulatory_class="promoter"
     CDS             4957..5670
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5682..6680
                     /label=TDH1 terminator
                     /note="TDH1 terminator"
                     /regulatory_class="terminator"
     promoter        6687..6907
                     /label=URA3 promoter
     rep_origin      7028..7273
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
       61 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
      121 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
      181 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
      241 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
      301 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      361 ctttttacgg ttcctggcct tttgctggcc ttttgctcat gatataatta aattgaagct
      421 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
      481 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
      541 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
      601 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
      661 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
      721 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
      781 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
      841 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
      901 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
      961 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     1021 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     1081 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     1141 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     1201 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     1261 gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag ttgggttaag
     1321 aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt
     1381 ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa
     1441 agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctagc
     1501 ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga ctatactaga
     1561 tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac gaggccttac
     1621 cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta agattctatc
     1681 ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa aaggcacttc
     1741 tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat gatattcgaa
     1801 tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt tacgatcgta
     1861 cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata
     1921 gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta
     1981 tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc
     2041 cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat
     2101 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga
     2161 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa
     2221 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc taatttttca
     2281 aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag cgctatttta
     2341 ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag agcgctattt
     2401 ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata atgcagtctc
     2461 ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt tggtgtctat
     2521 tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta ctagcgaagc
     2581 tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc gatgtggatt
     2641 gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt cagaaaatta
     2701 tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt acattttcgt
     2761 attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct aaagagtaat
     2821 actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca aggagcgaaa
     2881 ggtggatggg taggttatat agggatatag cacagagata tatagcaaag agatactttt
     2941 gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt gataatcaga
     3001 aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt ctttcctgcg
     3061 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3121 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gggtctgacg
     3181 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     3241 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3301 aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     3361 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     3421 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     3481 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     3541 catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg agcctggcga
     3601 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     3661 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     3721 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     3781 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     3841 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     3901 ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg
     3961 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     4021 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     4081 aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat tattgaagca
     4141 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4201 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcggacgga tcgcttgcct
     4261 gtaacttaca cgcgcctcgt ggcgcgcccc tgcaggattt aaatggccgg ccgtttaaac
     4321 cttgtacacc taccggtcac cagccaacaa tgtgcggatg gcgttacaac tttagtaaaa
     4381 atgtgcgcac cacaagccta catgactcca cgtcacatga aaccacaccg tggggccttg
     4441 ttgcgctagg aataggatat gcgacgaaga cgcttctgct tagtaaccac accacatttt
     4501 cagggggtcg atctgcttgc ttcctttact gtcacgagcg gcccataatc gcgctttttt
     4561 tttaaaaggc gcgagacagc aaacaggaag ctcgggtttc aaccttcgga gtggtcgcag
     4621 atctggagac tggatcttta caatacagta aggcaagcca ccatctgctt cttaggtgca
     4681 tgcgacggta tccacgtgca gaacaacata gtctgaagaa gggggggagg agcatgttca
     4741 ttctctgtag cagtaagagc ttggtgataa tgaccaaaac tggagtctcg aaatcatata
     4801 aatagacaat atattttcac acaatgagat ttgtagtaca gttctattct ctctcttgca
     4861 taaataagaa attcatcaag aacttggttt gatatttcac caacacacac aaaaaacagt
     4921 acttcactaa atttacacac aaaacaaagc ggccgcatgt ctaaaggtga agaattattc
     4981 actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca caaattttct
     5041 gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa atttatttgt
     5101 actactggta aattgccagt tccatggcca accttagtca ctactttcgg ttatggtgtt
     5161 caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa gtctgccatg
     5221 ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa ctacaagacc
     5281 agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt aaaaggtatt
     5341 gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta taactctcac
     5401 aatgtttaca tcatggctga caaacaaaag aatggtatca aagttaactt caaaattaga
     5461 cacaacattg aagatggttc tgttcaatta gctgaccatt atcaacaaaa tactccaatt
     5521 ggtgatggtc cagtcttgtt accagacaac cattacttat ccactcaatc tgccttatcc
     5581 aaagatccaa acgaaaagag agaccacatg gtcttgttag aatttgttac tgctgctggt
     5641 attacccatg gtatggatga attgtacaaa taagcggccg cataaagcaa tcttgatgag
     5701 gataatgatt tttttttgaa tatacataaa tactaccgtt tttctgctag attttgtgaa
     5761 gacgtaaata agtacatatt actttttaag ccaagacaag attaagcatt aactttaccc
     5821 ttttctcttc taagtttcaa tactagttat cactgtttaa aagttatggc gagaacgtcg
     5881 gcggttaaaa tatattaccc tgaacgtggt gaattgaagt tctaggatgg tttaaagatt
     5941 tttccttttt gggaaataag taaacaatat attgctgcct ttgcaaaacg cacataccca
     6001 caatatgtga ctattggcaa agaacgcatt atcctttgaa gaggtggata ctgatactaa
     6061 gagagtctct attccggctc cacttttagt ccagagatta cttgtcttct tacgtatcag
     6121 aacaagaaag catttccaaa gtaattgcat ttgcccttga gcagtatata tatactaaga
     6181 aaggaatcat actctcttat gtgccataat tctgttagga gcggaaataa ggctggtttt
     6241 ttggggatca agttcggaag tgctcttctc tcaattgcca caggtgccat tgctatcgca
     6301 ttgctatgca agttccacga ccacgaagcg gtactgatag ttattgtatg ctcgacattg
     6361 ttatatggaa taccgtcact gatatcgttt atcacagaga ctgtttttgc gcctagcaag
     6421 tttcatattg gatactttta caacgttctt aattttgcat tgccactgat caccatgggc
     6481 tgcacagtgg actattttca taataccttg aggagcccaa tatcggtaca aagcgagtct
     6541 cacagagtct atataacaac tctggattct ttgttaattt tcacactttt cattaatggc
     6601 atacaattag gattcttcct gaaggatggc aatgcgaaca atttcggcag ctcatctaac
     6661 aatattagta cggatcaata tagcttttca attcaattca tcattttttt tttattcttt
     6721 tttttgattt cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga
     6781 acgaaggaag gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac
     6841 atgaaattgc ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag
     6901 ataaatcatg tcgaaagcta catataagga acgtgctgct actcatccta gtcctgtttg
     6961 agatttaaat ggcgcgccgt ttaaaccctg caggggccgg ccccgtagaa aagatcaaag
     7021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7261 tggctgctgc cag
//
