LOCUS       40924_34241        6938 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_017_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6938)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6938)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(469..1269)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1270..1490)
                     /label=URA3 promoter
     rep_origin      complement(2089..2969)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3318..4109)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4110..4214)
                     /label=AmpR promoter
     regulatory      4370..5110
                     /label=IME2 promoter
                     /note="IME2 promoter"
                     /regulatory_class="promoter"
     CDS             5119..5832
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5844..6343
                     /label=IME2 terminator
                     /note="IME2 terminator"
                     /regulatory_class="terminator"
     promoter        6350..6570
                     /label=URA3 promoter
     rep_origin      6691..6938
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
       61 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      121 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      181 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      241 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      301 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      361 ttttacggtt cctggccttt tgctggcctt ttgctcatga tataattaaa ttgaagctct
      421 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc
      481 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc
      541 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac
      601 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac
      661 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc
      721 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata
      781 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg
      841 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac
      901 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg
      961 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta
     1021 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat
     1081 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa
     1141 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat
     1201 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc
     1261 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa
     1321 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct
     1381 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaaag
     1441 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctagctt
     1501 atcgatgata agctgtcaaa gatgagaatt aattccacgg actatagact atactagata
     1561 ctccgtctac tgtacgatac acttccgctc aggtccttgt cctttaacga ggccttacca
     1621 ctcttttgtt actctattga tccagctcag caaaggcagt gtgatctaag attctatctt
     1681 cgcgatgtag taaaactagc tagaccgaga aagagactag aaatgcaaaa ggcacttcta
     1741 caatggctgc catcattatt atccgatgtg acgctgcagc ttctcaatga tattcgaata
     1801 cgctttgagg agatacagcc taatatccga caaactgttt tacagattta cgatcgtact
     1861 tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt
     1921 atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt
     1981 tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg
     2041 gttcattttc tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct
     2101 gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     2161 ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga
     2221 atctgtgctt catttttgta aaacaaaaat gcaacgcgac gagagcgcta atttttcaaa
     2281 caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc
     2341 aacaaagaat ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt
     2401 ctaacaaagc atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt
     2461 gataactttt tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt
     2521 tctcttccat aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg
     2581 cgggtgcatt ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc
     2641 gcatactttg tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg
     2701 aacggtttct tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat
     2761 tgttttcgat tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac
     2821 tagagataaa cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg
     2881 tggatgggta ggttatatag ggatatagca cagagatata tagcaaagag atacttttga
     2941 gcaatgtttg tggaagcggt attcgcaatg ggaagctcca ccccggttga taatcagaaa
     3001 agccccaaaa acaggaagat tgtataagca aatatttaaa ttgtaattct ttcctgcgtt
     3061 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     3121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagg gtctgacgct
     3181 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3241 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3301 acttggtctg acagtcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     3361 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     3421 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     3481 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     3541 tcgtcatggg tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac
     3601 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     3661 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     3721 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     3781 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     3841 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     3901 agccacgata gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc
     3961 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     4021 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     4081 cctgcgtgca atccatcttg ttcaatcata ctcttccttt ttcaatatta ttgaagcatt
     4141 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     4201 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg cggacggatc gcttgcctgt
     4261 aacttacacg cgcctcgtgg cgcgcccctg caggatttaa atggccggcc gtttaaacct
     4321 tgtacaccta ccggtcacca gccaacaatg tgcggatggc gttacaactg atacctattc
     4381 tctcagtagc tgtttcacca agactccgca ctcatggtaa gtagaaaaag aaaactctca
     4441 caaaaatctg agtggcacag cttttccaaa tacgcttttt aaacttggag aaataaagat
     4501 gtattacccg ctggtgcctt aaaatatcct tttctccggt tgtccaataa tttatgttac
     4561 ggcggctatt tgagtttttg tggaaatctc tatcattaat aaatgtcagc cttgtgtctt
     4621 aaatgcaatt caacagggaa aaggcgcgag acttcggcgg caaaaagcca cagacaagtg
     4681 ctggttgcaa agaggtaaag aagtaatgtg tgtaatcaaa tagatatatt aaggtagcaa
     4741 ctcaccggtc tgtccatttt cttttcctga ataacttctt ctgtcttcac acattcattt
     4801 catttttatt ttccaatatc actcaacgag aaagaagtta caagatctat ctttcaaaat
     4861 agtttacagt tacctttact ttaccctttc ccttttcagt ttgttacccc ttctcctaaa
     4921 agttttattt tttgggtaaa gatataaagg acactccagt cggttaaggt ggctgtctag
     4981 agaatataaa cctgtattta tttaccaggc ttatcagaaa agttaatcgt gttcttaaat
     5041 attcattgaa aaggaacgtg atacagacgt aataaaagaa gagtactaag cttaacttaa
     5101 taggtcattt gcggccgcat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt
     5161 ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa
     5221 ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg taaattgcca
     5281 gttccatggc caaccttagt cactactttc ggttatggtg ttcaatgttt tgctagatac
     5341 ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa
     5401 gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt
     5461 gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt
     5521 aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta catcatggct
     5581 gacaaacaaa agaatggtat caaagttaac ttcaaaatta gacacaacat tgaagatggt
     5641 tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg
     5701 ttaccagaca accattactt atccactcaa tctgccttat ccaaagatcc aaacgaaaag
     5761 agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat
     5821 gaattgtaca aataagcggc cgctaatttg tcatttgact gacgaatcgt taggcacagt
     5881 tatatagtta tatacacata gacatacata tgttcgcata tatattatat tactatgcat
     5941 tcacctaaat agctactgct ctattaatac agtgcttttt cgatggcttt aatttaactt
     6001 attagacatt actttgcgtt tattcccctc caatatattt ttatgtttgt ttttcttgaa
     6061 cattttatga tttcttgctc ttggcggcta ctgattttgt taagcaactc atcaagaggt
     6121 caaaaaaagt agaacgagat ctttgtgttc ggttacccgg ctcagatcct aacttggtat
     6181 gtttattcgt ataagttact gttgtccaca ggcaatactc tgcagaaaat taaaacggca
     6241 ttaatgctag gacaaccaga actgttacta ctgtatgtgc gatagttgat aactgcaaca
     6301 ttatgcccgg tatattctca aaaaacccta ttactgcata cgatagcttt tcaattcaat
     6361 tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg
     6421 gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
     6481 cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
     6541 acaaaaacct gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct
     6601 gctactcatc ctagtcctgt ttgagattta aatggcgcgc cgtttaaacc ctgcaggggc
     6661 cggccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6721 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6781 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6841 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6901 cgctctgcta atcctgttac cagtggctgc tgccagtg
//
