LOCUS       40924_34236        6265 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_016_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6265)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6265)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(478..1278)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1279..1499)
                     /label=URA3 promoter
     rep_origin      complement(2098..2978)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3327..4118)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4119..4223)
                     /label=AmpR promoter
     regulatory      4379..4666
                     /label=CYC1 promoter
                     /note="CYC1 promoter"
                     /regulatory_class="promoter"
     CDS             4675..5388
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     terminator      5425..5674
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        5686..5906
                     /label=URA3 promoter
     rep_origin      6027..6265
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
       61 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcatgat ataattaaat
      421 tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt
      481 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
      541 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
      601 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
      661 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
      721 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
      781 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
      841 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
      901 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
      961 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
     1021 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
     1081 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
     1141 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
     1201 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
     1261 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
     1321 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
     1381 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
     1441 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
     1501 agctagctta tcgatgataa gctgtcaaag atgagaatta attccacgga ctatagacta
     1561 tactagatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     1621 gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg tgatctaaga
     1681 ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga aatgcaaaag
     1741 gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct tctcaatgat
     1801 attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac
     1861 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     1921 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     1981 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     2041 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     2101 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     2161 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     2221 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgacg agagcgctaa
     2281 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     2341 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     2401 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     2461 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     2521 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     2581 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     2641 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     2701 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     2761 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
     2821 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
     2881 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
     2941 tacttttgag caatgtttgt ggaagcggta ttcgcaatgg gaagctccac cccggttgat
     3001 aatcagaaaa gccccaaaaa caggaagatt gtataagcaa atatttaaat tgtaattctt
     3061 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     3121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg
     3181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3301 tatgagtaaa cttggtctga cagtcagaag aactcgtcaa gaaggcgata gaaggcgatg
     3361 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     3421 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     3481 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     3541 aagcaggcat cgtcatgggt cacgacgaga tcctcgccgt cgggcatgct cgccttgagc
     3601 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     3661 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     3721 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     3781 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     3841 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     3901 gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca gttcattcag ggcaccggac
     3961 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     4021 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     4081 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatac tcttcctttt tcaatattat
     4141 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4201 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgc ggacggatcg
     4261 cttgcctgta acttacacgc gcctcgtggc gcgcccctgc aggatttaaa tggccggccg
     4321 tttaaacctt gtacacctac cggtcaccag ccaacaatgt gcggatggcg ttacaactag
     4381 cgttggttgg tggatcaagc ccacgcgtag gcaatcctcg agcagatccg ccaggcgtgt
     4441 atatatagcg tggatggcca ggcaacttta gtgctgacac atacaggcat atatatatgt
     4501 gtgcgacgac acatgatcat atggcatgca tgtgctctgt atgtatataa aactcttgtt
     4561 ttcttctttt ctctaaatat tctttcctta tacattagga cctttgcagc ataaattact
     4621 atacttctat agacacacaa acacaaatac acacactaaa ttaatagcgg ccgcatgtct
     4681 aaaggtgaag aattattcac tggtgttgtc ccaattttgg ttgaattaga tggtgatgtt
     4741 aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg atgctactta cggtaaattg
     4801 accttaaaat ttatttgtac tactggtaaa ttgccagttc catggccaac cttagtcact
     4861 actttcggtt atggtgttca atgttttgct agatacccag atcatatgaa acaacatgac
     4921 tttttcaagt ctgccatgcc agaaggttat gttcaagaaa gaactatttt tttcaaagat
     4981 gacggtaact acaagaccag agctgaagtc aagtttgaag gtgatacctt agttaataga
     5041 atcgaattaa aaggtattga ttttaaagaa gatggtaaca ttttaggtca caaattggaa
     5101 tacaactata actctcacaa tgtttacatc atggctgaca aacaaaagaa tggtatcaaa
     5161 gttaacttca aaattagaca caacattgaa gatggttctg ttcaattagc tgaccattat
     5221 caacaaaata ctccaattgg tgatggtcca gtcttgttac cagacaacca ttacttatcc
     5281 actcaatctg ccttatccaa agatccaaac gaaaagagag accacatggt cttgttagaa
     5341 tttgttactg ctgctggtat tacccatggt atggatgaat tgtacaaata agcggccgca
     5401 caggcccctt ttcctttgtc gatatcatgt aattagttat gtcacgctta cattcacgcc
     5461 ctcctcccac atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc
     5521 ctatttattt tttttaatag ttatgttagt attaagaacg ttatttatat ttcaaatttt
     5581 tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga
     5641 gaaggttttg ggacgctcga aggctttaat ttgcaagctt agcttttcaa ttcaattcat
     5701 catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa
     5761 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca
     5821 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa
     5881 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     5941 ctcatcctag tcctgtttga gatttaaatg gcgcgccgtt taaaccctgc aggggccggc
     6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6241 ctgctaatcc tgttaccagt ggctg
//
