LOCUS       40924_34231        6653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pPC_015_GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6653)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     AssemblX: a user-friendly toolkit for rapid and reliable multi-gene 
            assemblies
  JOURNAL   Nucleic Acids Res. (2017) In press
  PUBMED    28130422
REFERENCE   2  (bases 1 to 6653)
  AUTHORS   Hochrein L, Machens F, Gremmels J, Schulz K, Messerschmidt K, 
            Mueller-Roeber B.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-NOV-2016) Department of Molecular Biology - Cell2Fab 
            research unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, 
            Potsdam, Brandenburg 14476, Germany
REFERENCE   3  (bases 1 to 6653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-NOV-2016) Department of Molecular Biology - Cell2Fab research 
            unit, University of Potsdam, Karl-Liebknecht-Str. 24-25, Potsdam, 
            Brandenburg 14476, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(481..1281)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1282..1502)
                     /label=URA3 promoter
     rep_origin      complement(2101..2981)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(3330..4121)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(4122..4226)
                     /label=AmpR promoter
     regulatory      4382..5050
                     /label=ACT1 promoter
                     /note="ACT1 promoter"
                     /regulatory_class="promoter"
     CDS             5059..5772
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      5784..6070
                     /label=ACT1 terminator
                     /note="ACT1 terminator"
                     /regulatory_class="terminator"
     promoter        6077..6297
                     /label=URA3 promoter
     rep_origin      6418..6653
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
       61 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
      121 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
      181 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      241 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      301 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      361 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcat gatataatta
      421 aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta
      481 gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc
      541 ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga
      601 tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc
      661 atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat
      721 gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt
      781 acccttagta tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa
      841 aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc
      901 tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc
      961 cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag
     1021 taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa
     1081 atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac
     1141 taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt
     1201 ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc
     1261 cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg ttctgtgcag
     1321 ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc gtatatatac
     1381 caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta ccgaatcaaa
     1441 aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg
     1501 aaaagctagc ttatcgatga taagctgtca aagatgagaa ttaattccac ggactataga
     1561 ctatactaga tactccgtct actgtacgat acacttccgc tcaggtcctt gtcctttaac
     1621 gaggccttac cactcttttg ttactctatt gatccagctc agcaaaggca gtgtgatcta
     1681 agattctatc ttcgcgatgt agtaaaacta gctagaccga gaaagagact agaaatgcaa
     1741 aaggcacttc tacaatggct gccatcatta ttatccgatg tgacgctgca gcttctcaat
     1801 gatattcgaa tacgctttga ggagatacag cctaatatcc gacaaactgt tttacagatt
     1861 tacgatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg agttttccct
     1921 gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt tacggaagac
     1981 aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt aatcttgcac
     2041 gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca tatctttgtt
     2101 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     2161 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     2221 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg acgagagcgc
     2281 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag
     2341 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag
     2401 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata
     2461 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt
     2521 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta
     2581 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc
     2641 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt
     2701 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt
     2761 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct
     2821 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca
     2881 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag
     2941 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tgggaagctc caccccggtt
     3001 gataatcaga aaagccccaa aaacaggaag attgtataag caaatattta aattgtaatt
     3061 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3121 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3241 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3301 atatatgagt aaacttggtc tgacagtcag aagaactcgt caagaaggcg atagaaggcg
     3361 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     3421 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     3481 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     3541 ggcaagcagg catcgtcatg ggtcacgacg agatcctcgc cgtcgggcat gctcgccttg
     3601 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     3661 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     3721 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     3781 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     3841 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     3901 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt gcagttcatt cagggcaccg
     3961 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     4021 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     4081 gcggccggag aacctgcgtg caatccatct tgttcaatca tactcttcct ttttcaatat
     4141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgcggacgga
     4261 tcgcttgcct gtaacttaca cgcgcctcgt ggcgcgcccc tgcaggattt aaatggccgg
     4321 ccgtttaaac cttgtacacc taccggtcac cagccaacaa tgtgcggatg gcgttacaac
     4381 tacaagcgcg cctctacctt gcagacccat ataatataat aactaaataa gtaaataaga
     4441 cacacgcgag aacatatata cacaattaca gtaacaataa caagaggaca gatactacca
     4501 aaatgtgtgg ggaagcgggt aagctgccac agcaattaat gcacaacatt taacctacat
     4561 tcttccttat cggatcctca aaacccttaa aaacatatgc ctcaccctaa catattttcc
     4621 aattaaccct caatatttct ctgtcacccg gcctctattt tccattttct tctttacccg
     4681 ccacgcgttt ttttctttca aatttttttc ttccttcttc tttttcttcc acgtcctctt
     4741 gcataaataa ataaaccgtt ttgaaaccaa actcgcctct ctctctcctt tttgaaatat
     4801 ttttgggttt gtttgatcct ttccttccca atctctcttg tttaatatat attcatttat
     4861 atcacgctct ctttttatct tccttttttt cctctctctt gtattcttcc ttcccctttc
     4921 tactcaaacc aagaagaaaa agaaaaggtc aatctttgtt aaagaatagg atcttctact
     4981 acatcagctt ttagattttt cacgcttact gcttttttct tcccaagatc gaaaatttac
     5041 tgaattaaca gcggccgcat gtctaaaggt gaagaattat tcactggtgt tgtcccaatt
     5101 ttggttgaat tagatggtga tgttaatggt cacaaatttt ctgtctccgg tgaaggtgaa
     5161 ggtgatgcta cttacggtaa attgacctta aaatttattt gtactactgg taaattgcca
     5221 gttccatggc caaccttagt cactactttc ggttatggtg ttcaatgttt tgctagatac
     5281 ccagatcata tgaaacaaca tgactttttc aagtctgcca tgccagaagg ttatgttcaa
     5341 gaaagaacta tttttttcaa agatgacggt aactacaaga ccagagctga agtcaagttt
     5401 gaaggtgata ccttagttaa tagaatcgaa ttaaaaggta ttgattttaa agaagatggt
     5461 aacattttag gtcacaaatt ggaatacaac tataactctc acaatgttta catcatggct
     5521 gacaaacaaa agaatggtat caaagttaac ttcaaaatta gacacaacat tgaagatggt
     5581 tctgttcaat tagctgacca ttatcaacaa aatactccaa ttggtgatgg tccagtcttg
     5641 ttaccagaca accattactt atccactcaa tctgccttat ccaaagatcc aaacgaaaag
     5701 agagaccaca tggtcttgtt agaatttgtt actgctgctg gtattaccca tggtatggat
     5761 gaattgtaca aataagcggc cgctctctgc ttttgtgcgc gtatgtttat gtatgtacct
     5821 ctctctctat ttctattttt aaaccaccct ctcaataaaa taaaaataat aaagtatttt
     5881 taaggaaaag acgtgtttaa gcactgactt tatctacttt ttgtacgttt tcattgatat
     5941 aatgtgtttt gtctctccct tttctacgaa aatttcaaaa attgaccaaa aaaaggaata
     6001 tatatacgaa aaactattat atttatatat catagtgttg ataaaaaatg tttatccatt
     6061 ggaccgtgta tagcttttca attcaattca tcattttttt tttattcttt tttttgattt
     6121 cggtttcttt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag
     6181 gagcacagac ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc
     6241 ccagtattct taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg
     6301 tcgaaagcta catataagga acgtgctgct actcatccta gtcctgtttg agatttaaat
     6361 ggcgcgccgt ttaaaccctg caggggccgg ccccgtagaa aagatcaaag gatcttcttg
     6421 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6481 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6541 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6601 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tgg
//
